Peanut mutant creation method and application

A mutant, peanut technology, applied in the direction of applications, botany equipment and methods, angiosperms/flowering plants, etc.

Inactive Publication Date: 2020-09-18
SHANDONG PEANUT RES INST
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  • Abstract
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  • Application Information

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Problems solved by technology

However, although some excellent peanut mutant individuals with trait variation have been obtained, most of them are identified for the mutagenesis of a certain trait

Method used

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  • Peanut mutant creation method and application
  • Peanut mutant creation method and application
  • Peanut mutant creation method and application

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Experimental program
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Embodiment Construction

[0027] In order to make it easier for those skilled in the art to understand the present invention, the specific implementation manners of the present invention will be described below with reference to the accompanying drawings.

[0028] 1 Materials and methods

[0029] 1.1 Test material

[0030] The mutagenic material Huayu 22 was preserved by Shandong Peanut Research Institute. EMS reagents were purchased from Sigma, USA.

[0031] 1.2 Test method

[0032] 1.2.1 Exploration of peanut EMS mutagenesis conditions

[0033] Weigh 40 g of the seeds of Huayu No. 22 and place them in a 500 mL Erlenmeyer flask. Add 200 mL of double-distilled water to the Erlenmeyer flask containing the seeds, then use a pipette to suck out 800 μL of double-distilled water and add an equal volume of EMS reagent, so that the final concentration of the EMS solution in the bottle is 0.4%. Shaker (25°C, 105rpm) for 10h. A total of about 5000 seeds were processed. After the treatment, the seeds were...

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Abstract

The invention relates to a peanut mutant creation method and application. The peanut mutant creation method can be used for creating peanut mutant groups with rich phenotypes. The peanut mutant creation method comprises the following steps: (1) soaking peanut seeds with an EMS (ethyl methane sulfonate) aqueous solution with the concentration of 0.4-0.5% and treating the peanut seeds for 8-10 h through a shaking table; (2) washing the seeds with running water for 3-5 h and removing residual EMS; (3) germinating the treated seeds in an illumination culture box; (4) picking out the germinated andwhite seeds and planting the seeds; (5) planting M1 generations; in a peanut growth period, investigating the phenotypes of mutants and counting the ratio of the various mutant phenotypes to the groups; and (6) randomly selecting one seed from each single plant of M2 generations and planting the; investigating the phenotypes of the mutants and counting the ratio of the various mutant phenotypes to the groups to obtain peanut EMS mutant groups with the rich phenotypes. By adopting the peanut mutant creation method, the EMS mutation groups with variation of plant height, plant type, branch number, pod size and the like can be obtained.

Description

technical field [0001] The invention relates to a method for creating peanut mutants and its application. The method can be used to create peanut mutant populations with rich variant phenotypes. Background technique [0002] Peanut is an important oil crop and economic crop in my country, and it is also a major export-earning agricultural product. Cultivating high-yielding, high-quality, special-purpose new peanut varieties has always been the most important research direction for peanut breeders. At present, the main means of peanut breeding is hybrid breeding. However, due to the poor specificity of the peanut varieties bred in my country, most of the gene sources come from "Fuhuasheng" and "Liontouqi", and the available germplasm resources are limited. It is difficult to improve the breeding efficiency with traditional hybrid breeding-based technical means and existing germplasm resources. Mutagenesis technology has many advantages such as high mutation efficiency, wid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/06A01H6/54
CPCA01H1/04A01H1/06A01H6/541
Inventor 苑翠玲单世华孙全喜李春娟闫彩霞赵小波王娟张浩
Owner SHANDONG PEANUT RES INST
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