A kind of rice senescence control gene osckx11 and its application

Abstract

The invention belongs to the technical field of genetic engineering and discloses a rice leaf senescence control gene OsCKX11 and its application. The amino acid sequence of the rice leaf senescence control gene OsCKX11 has at least 70% homology with the amino acid sequence shown in the amino acid sequence SEQ ID No.1 sex. The present invention conducts site-directed mutation of rice cytokinin oxidase coding gene OsCKX11, analyzes the phenotypic changes of its mutants, and finds that the lack of function of this gene will cause late senescence of rice leaves; in some premature senescence cultivars, OsCKX11 is knocked out by genetic engineering technology It can purposely regulate the aging process of plants and adjust the development period of plant populations to achieve the purpose of increasing production, which has very important application value.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a rice aging control gene OsCKX11 and its application. The invention uses the reverse genetics technology to identify the biological function of OsCKX11, and also relates to inhibiting the premature senescence of rice leaves after the gene mutation, thereby regulating the development of plants and affecting the yield of rice. Background technique [0002] Leaf senescence is the last stage of leaf growth and development. Leaf senescence is a complex physiological activity. Some proteins are activated and expressed while others are degraded[1]. When the leaves are aging, as the chlorophyll is degraded and the water in the body is reduced, the photosynthetic rate gradually decreases, and the nutrients are transported to the fruits or seeds for storage. Relevant studies have shown that when the leaves are in a senescent state, the content of endogenous cytoki...

AI Technical Summary

Problems solved by technology

[0017] In summary, the existing problems in the prior art are: in the process of conventional rice breeding, super rice breeding and hybrid breeding in current agricultural production, there is a phenomenon of premature senescence of leaves, which seriously affects the filling of seeds, and then affects the yield of rice. and quality

However, the method of inhibiting rice leaf senescence by improving rice cytokinin content has not been reported yet.

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