Rice senescence control gene OsCKX11 and application thereof

A gene and senescence technology, applied to the rice senescence control gene OsCKX11 and its application fields, can solve the problems of affecting seed filling, affecting rice yield and quality, premature leaf senescence, etc., and achieving the effect of important application value

Active Publication Date: 2019-03-01
ZHEJIANG NORMAL UNIVERSITY
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Problems solved by technology

[0018] In the process of conventional rice breeding, super rice breeding and hybrid breeding in current agricultural production, there is a phenomenon of premature leaf senescence, which seriously affects the filling of seeds, which in turn affects the yield and quality of rice
[0019] However, the method of inhibiting rice leaf senescence by improving rice cytokinin content has not been reported yet.

Method used

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  • Rice senescence control gene OsCKX11 and application thereof
  • Rice senescence control gene OsCKX11 and application thereof
  • Rice senescence control gene OsCKX11 and application thereof

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Embodiment Construction

[0041] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0042] In the present invention, through point-directed mutation of rice cytokinin oxidase coding gene OsCKX11, and analyzing the phenotype changes of the mutants, it is found that the loss of the gene function will lead to late senescence of rice leaves. In some premature senescence cultivars, using genetic engineering technology to knock out OsCKX11 can purposely regulate the leaf senescence process of plants and adjust the developmental period of plant populations to achieve the purpose of increasing production, which has very important application value.

[0043] The rice senescence control gene OsCKX11 provided in the ...

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Abstract

The invention belongs to the technical field of gene engineering and discloses a rice senescence control gene OsCKX11 and application thereof. An amino acid sequence of the rice leaf senescence control gene OsCKX11 and an amino acid sequence shown as SEQ ID NO.2 are homological by at least 70%. By site-directed mutagenesis of rice cytokinin oxidase coding gene OsCKX11 and mutant phenotypic changeanalysis, the fact that the gene functional deficiency results in rice leaf senescence delaying; in certain premature senescence cultivation varieties, knocking out OsCKX11 by the gene engineering technology enables purposive regulation of a plant senescence process, yield increasing is realized by regulation of a plant population development stage, and a significant application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a rice aging control gene OsCKX11 and its application. The invention uses the reverse genetics technology to identify the biological function of OsCKX11, and also relates to inhibiting the premature senescence of rice leaves after the gene mutation, thereby regulating the development of plants and affecting the yield of rice. Background technique [0002] Leaf senescence is the last stage of leaf growth and development. Leaf senescence is a complex physiological activity. Some proteins are activated and expressed while others are degraded. [1] . When the leaves are aging, as the chlorophyll is degraded and the water in the body is reduced, the photosynthetic rate gradually decreases, and the nutrients are transported to the fruits or seeds for storage. Relevant studies have shown that when leaves are in a senescent state, the content of endogenous cytoki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/06C12N15/53C12N15/82C12N1/21A01H5/00A01H6/46
CPCC12N9/0026C12N15/8241C12Y105/99012
Inventor 张可伟崔付斌赵江哲章薇彭凯轩张艳军
Owner ZHEJIANG NORMAL UNIVERSITY
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