92results about How to "High in lysine" patented technology

The invention relates to a method for producing high lysine fermented soybean meal. The method comprises the following steps: a, preparing a soybean meal raw material; b, adding water into the soybean meal raw material and stirring up uniformly to obtain aqueous soybean meal slurry; c, carrying out germicidal treatment for the aqueous soybean meal slurry obtained from step b; d, adding urea and ammonium sulphate into the aqueous soybean meal slurry treated through step c, and stirring up uniformly to obtain a soybean meal culture medium; e, accessing microbial strains capable of generating lysine into the soybean meal culture medium obtained from step d; f, conducting temperature control fermentation for the soybean meal culture medium treated through step e to obtain fermented soybean meal; and g, drying the fermented soybean meal to obtain the final product. The invention has the advantages that the preparation method brings out an apparent increase in the nutrient substances present in the fermented product with the content of soybean meal protein rising by more than 5%, and an obvious boost in the content of lysine, which increases to over 4.0% under the action of microzyme, from an initial 2.8%.

The invention discloses eel bone, barbary wolfberry and water spinach juice noodles. The noodles are prepared from raw materials in parts by weight as follows: 300-350 parts of flour, 30-33 parts of gluten powder, 9-20 parts of pollen, 13-16 parts of corn peel dietary fiber, 28-35 parts of eel bones, 5-6 parts of konjac flour, 23-25 parts of barbary wolfberry fruit powder, 20-22 parts of water spinach, 3-4 parts of condensed milk, 2-3.2 parts of sharpleaf galangal fruits, 0.4-1.3 parts of palmleaf raspberry fruits, 2-2.4 parts of elm seeds and proper amounts of table salt and papain. The noodles taste fragrant and soft, are chewy and have good elasticity, and food and medicinal materials such as oroxylum seeds, Murdannia simplex, corydalis roots and the like are also added, so that the noodles have health-care functions of nourishing yin, clearing heat, moistening the lung, soothing the liver, harmonizing the stomach and tonifying the spleen.

This invention relates to four chimeric genes, a first encoding lysine-insensitive aspartokinase (AK), which is operably linked to a plant chloroplast transit sequence, a second encoding lysine-insensitive dihydrodipicolinic acid synthase (DHDPS), which is operably linked to a plant chloroplast transit sequence, a third encoding a lysine-rich protein, and a fourth encoding a plant lysine ketoglutarate reductase, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of lysine or threonine in the seeds of transformed plants are provided. Also provided are transformed plants wherein the seeds accumulate lysine or threonine to higher levels than untransformed plants.

The invention discloses a grain milk and a manufacturing method thereof. The grain milk is prepared from the following raw materials in parts by weight: 30-50 parts of sesame, 20-40 parts of red bean, 400-600 parts of fresh milk, 200-300 parts of water, and 10-30 parts of semen coicis. The manufacturing method comprises the following steps: 1) putting 30-50 parts by weight of sesame, 20-40 parts by weight of red bean, 10-30 parts by weight of semen coicis and 300 parts by weight of water into an agitator for pulping, and filtering to obtain a mixed liquid; and 2) uniformly mixing the obtained mixed liquid and 400-600 parts by weight of milk, boiling the mixture with small fire, and waiting for cooling down to obtain the grain milk. The preparation method of the grain milk is simple, and the grain milk is high in nutritive value, does not contain any chemical additive, has rich tastes, and is a green and delicious healthy drink.

The present disclosure relates to high protein dietary supplements for treating various symptoms and diseases associated with protein deficiency including weight gain, obesity, catabolic diseases, fibromyalgia, anxiety reactions, posttraumatic stress and chronic fatigue syndrome. Embodiments of dietary supplements comprise combinations of proteins, essential and semi-essential amino acids including L-Lysine, L-Arginine, and/or L-Histidine.

Disclosed herein are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase and a gene suppression molecule for lysine ketoglutarate reductase, the activity of which results in increased lysine in a plant seed.

The invention relates to a rabbit excrement fermented leaf fertilizer and a preparation method of the rabbit excrement fermented leaf fertilizer. The rabbit excrement fermented leaf fertilizer comprises the following components in parts by weight: 5-15 parts by weight of rabbit excrement, 0.1-0.4 part by weight of zinc sulfate, 0.01-0.05 part by weight of EM (Effective Microorganisms) stock solution and 50-90 parts by weight of water. The leaf fertilizer provided by the invention has multiple fertilizer efficiencies, can increase zinc content of crops, contents of zinc content, soluble sugar and lysine, can improve quality of the crops, has an effect of removing insect, and is capable of removing plant insect attack. Because of multiple functions of the leaf fertilizer provided by the invention, labor and economic expense in cultivation can be saved, and the production cost of crops is favorably lowered. The rabbit excrement fermented leaf fertilizer is completely prepared from green raw materials without bringing health hazard caused by pesticide residue.

The invention discloses a sweet potato porridge and a production method thereof; the raw material of the sweet potato porridge comprises raw sweet potatoes after aging treatment; the raw sweet potatoes after aging treatment are the sweet potatoes at the beginning of germination when the raw fresh sweet potatoes are placed in the embryonic environment. The production method comprises the following steps: (1) aging treatment of raw fresh potatoes, that is, the raw fresh sweet potatoes are stored at 16-20 DEG C and under a 85-90% relative humidity and turned into the raw sweet potatoes after aging treatment at the beginning of germination; (2) the raw potatoes obtained in step (1) are peeled, cut into small cubes and then cased in a jar; (3) rice is washed and put into the jar; (4) coarse cereals are washed and/or soaked and then put into the jar; (5) water is added into the jar, and a top gap is retained, following by the steps of mouth sealing, sterilizing, porridge boiling and cooling. The sweet potato porridge tastes delicious and unsour and has rich taste of sweet potato, without flatulence and hiccup after consumption.

The invention discloses jack fruit seed whole flour cookies and a preparing method thereof. The jack fruit seed whole flour cookies are prepared from, by weight, 110-130 parts of low-gluten flour, 50-80 parts of powdered sugar, 40-60 parts of eggs, 60-100 parts of jack fruit seeds and 60-80 parts of butter. According to the technical scheme, the jack fruit seed whole flour is utilized to produce the cookies, the jack fruit seed starch is low in water retention rate, the quality of the cookies is improved, and the additive amount of the butter is effectively reduced; the nutrients of the jack fruit seed whole flour are made full use of, the jack fruit seed whole flour is high in lysine content, the lack of lysine in grains is made up, the efficiency ratio of protein can be increased, and the protein contained in the jack fruit seed whole flour does not form a gluten network; the jack fruit seed flour is high in content of resistant starch, can effectively lower the glycemic index of the cookies, and has a very good health-care function; the jack fruit seed whole flour cookies are pure in scent, not sticky and loose in mouthfeel, can be produced in large scale, and can be widely circulated, and nutritional utilization of the jack fruit seeds is promoted.

The disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more floury2 (fl2) mutations. In embodiments, the disclosure concerns a gene specific PCR-based molecular (KASPar®) assay for identifying the floury2 trait in plants. In certain embodiments, compositions and methods are disclosed for determining the zygosity of corn plants with respect to the fl2 allele. In particular embodiments, the assay may be used for fl2 germplasm identification, accelerating introgression and molecular breeding programs in corn and other plants.

The invention discloses a compound fermented feed additive for egg-laying poultry as well as a preparation method and an application of the additive and belongs to the technical field of feed additives. The compound fermented feed additive for egg-laying poultry is prepared from components in parts by weight as follows: 22-25 parts of fermented sunflower seed meal, 15-20 parts of fermented alga powder, 2-4 parts of oyster powder, 1-3 parts of yellow mealworm powder, 0.05-0.1 part of soybean isoflavone, 0.1-0.3 part of carboxymethyl chitosan and 0.05-0.1 part of perilla oil. The compound fermented feed additive for egg-laying poultry can increase the egg laying rate of the poultry, improve the egg laying property and further improve the laid egg quality. The compound fermented feed additivecan promote digestive absorption of nutrient substances and enhance immunity by regulating internal environments of intestinal tracts of the poultry and promoting growth and propagation of probiotics.

The invention discloses a method for producing oyster mushrooms with the high lysine content through purple perilla straw. According to the method, after the purple perilla straw is mixed with lime water and fermented, the mixture is mixed with cottonseed hulls to manufacture an edible fungi compost, the edible fungi compost is sterilized at high temperature after being bagged, an oyster mushroom strain is inoculated, and cultivation management is carried out in a conventional method. Compared with oyster mushrooms cultivated through a traditional cottonseed hull compost, the oyster mushrooms cultivated in the method is remarkably improved in the content of lysine, has the advantages of being short in growth period, good in quality, high in yield, rich in nutrition and the like, can meet the requirement of the market for high-quality oyster mushrooms special for supplementing amino acid, and provides an efficient way for comprehensive utilization of purple perilla.

The invention discloses a tilapia feed. The tilapia feed comprises the following components, wherein the components comprise, by weight, 3-6 parts of hydrolyzed feather meal, 4-10 parts of soybean powder, 20-25 parts of cottonseed powder, 35-40 parts of rice, 5-10 parts of crude wheat powder, 5-10 parts of meat and bone meal, 6-10 parts of dried vinasse, 5-10 parts of peanut powder, 5-10 parts of a mineral premixing additive, and 2-6 parts of a vitamin premixing additive. According to the present invention, the formula of the tilapia feed of the present invention is scientific, the nutrition is balanced, and the tilapia can grow rapidly with the feed.

The invention discloses a chicken feed. The chicken feed comprises 1.5-3.5wt% of grass meal and 0.05-0.3wt% of plant extract as effective components; the grass meal is composed of corncob powder, alfalfa meal and purslane powder; and the plant extract is a green powdery substance prepared through the steps of crushing and pulping fresh corncobs and purslane, pressing through a presser, collecting the obtained juice, and drying. The chicken feed has nutritional and medicinal functions, improves the body immunity, reduces diseases in the breeding process, reduces the use of drugs, and makes safe animal products produced. The chicken feed reduces the respiratory diseases, vent picking, feather picking and other phenomena of chickens in the breeding process, improves the conversion rate of feeds, reduces the concentration of ammonia in excreta, improves the commodity, reduces the abdominal fat percentage, improves the quality of meat, saves the feeds, reduces the dosage, reduces the feeding cost, and improves the chicken raising benefit.

The invention provides a fertile transgenic monocot, e.g., maize, plant encoding an endosperm specific prolamin box binding factor peptide (PBF) that is expressed so as to increase the methionine and/or lysine content of the seeds of said plant over said content in the seeds of the corresponding plant that lacks said gene, wherein said corresponding plant was used to prepare said transgenic plant, or an ancestor of said transgenic plant.

The invention discloses an expression vector for increasing rice protein content and quality and its production method and application, having the structure and characteristics as a and b shown. The vector contains glutelin gene promoter, soybean Gy7 full length gene or Gy7 gene coding sequence cDNA fragment, terminator and incision enzyme sensitive sequence or marker gene sequence. The vector can be used for transforming Gramineae plants like rice to obtain rice variety containing high protein content and high restriction amino acid content.

The invention relates to the field of wheat breeding, in particular to a method for improving content of protein and combined lysine in wheat seeds, which guides high chili lysine protein gene (cflr) into conventional wheat by adopting a genetic engineering method and expresses the gene to improve the content of the protein and the combined lysine in the wheat seeds. The method is characterized by comprising the following steps of: constructing an expression vector pAHC25-Cf1r of the high chili lysine protein gene (cflr); guiding the pAHC25-Cf1r into an acceptor wheat embryo cell through a gene gun to perform callus induction and plant regeneration on the wheat embryo cell so as to obtain a T5-generation transgenic homozygous strain; and detecting cflr gene expression abundance in progeny seeds of the T5-generation transgenic homozygous strain, measuring the content of the protein and the combined lysine in the seeds, and screening the strains of which the content of the protein and the combined lysine in the seeds is remarkably improved. Compared with the conventional germ plasm breeding method, the method has the advantages of short breeding cycle, obvious improvement effect and little influence on important agronomic traits.

The invention provides a high-protein cassava feed. The feed comprises the following raw materials: a cassava solid culture medium and a culture solution. The invention also provides a preparation method of the feed. According to the high-protein cassava feed disclosed by the invention, cassava protein fermented by yeast is good in taste, and can obviously improve the defect of poor palatability of cassava, and improve the feed intake of animals. According to the high-protein cassava feed disclosed by the invention, the cassava protein with high protein content can be obtained by using a single strain fermentation mode. The method can fully utilize local feed resources, save production cost, and improve animal production.

The invention relates to construction of a high-lysine protein gene SB401 expression vector and application thereof and aims to solve the technical problems of efficient introduction of an exogenous high-lysine protein gene into a plant and efficient expression of the protein gene. The method comprises the following steps of: extracting ribonucleic acid (RNA) of a potato, obtaining full-length complementary deoxyribonucleic acid (CDNA) of the potato through reverse transcription, obtaining a sb401 gene by a polymerase chain reaction (PCR) method, recombining the gene and cloning the recombined gene into a plant dual-element expression vector; and introducing the gene into a corn gene group by an agrobacterium-mediated method, obtaining an expression transgenosis positive seedling and performing efficient expression so as to increase the lysine content of corn and further enhance the quality of corn. The method has unique advantages of low cost, small copy number, high repeatability, a small number of gene silencing phenomena, short transformation period, capability of transforming large fragments and the like.

This invention relates to the coagulation of protein containing nutritional compositions in the upper gastro-intestinal tract, more in particular in the stomach and provides a method for reducing such coagulation.

The invention provides a microbial organic fertilizer containing modified municipal sludge, and a preparation method thereof. The microbial organic fertilizer comprises bioactive peptides, modified municipal sludge, chicken bones, cottonseed powder, wheat bran, waste molasses, vinyltrimethoxy silane, Scenedesmus acuminatus, polyamine cross-linked cellulose resin, honeysuckle flower extract, auxin, biologic humic acid, earthworm globulin and a composite bacterium agent. The effective quantity of live bacteria in the microbial organic fertilizer prepared in the invention is 0.83-0.96 billion/g, the total nitrogen content is 7-10wt%, the total phosphorus content is 3-5wt%, the total potassium content is 8-11wt%, the content of organic matters is 52.6-53.9wt%, the water content is 2.0-2.5wt%, the particle strength is 10.8-11.5N, and the percentage of contaminating microorganism is 2.1-2.6%; and the microbial organic fertilizer can effectively improve the output of agricultural products and the content of nutrients, and has an insecticidal effect.