7 results about "Wheatear" patented technology

The invention discloses application of protein TaTDM and related biological materials thereof, and belongs to the field of plant breeding. The amino acid sequence of the protein TaTDM is as shown in SEQ ID NO: 2 or SEQ ID NO: 4 or SEQ ID NO: 6. The TaTDM gene knockout homozygous mutant strains tatdmm1 and tatdmm2 are obtained by carrying out gene knockout on wild type wheat Fielder, and it is found that at the temperature of 25 DEG C, the total floret number of each spike of the Fielder, the tatdmm1 and the tatdmm2 and the survival rate under the water shortage treatment condition are remarkably higher than those of the wild type wheat Fielder. The protein TaTDM has an important application value in regulation and control of wheat ear type characters and stress tolerance, provides a high-quality germplasm resource, and is of great significance in cultivation of drought-enduring plant varieties and improvement of crop yield.

The application relates to the technical field of image processing, in particular to an intelligent detection system for wheat ear diseases and insect pests in a field. The system comprises: an image acquisition module: acquiring a wheat ear image in a field; an illumination analysis module: constructing an illumination influence coefficient of each pixel point based on the brightness value distribution in the neighborhood pixel block of the pixel point; a gray scale analysis module: determining a feature influence coefficient of each pixel point in the neighborhood pixel block based on the gray scale value distribution in the neighborhood of the pixel point; an image enhancement module: adaptively adjusting a preset basic attenuation parameter based on the feature influence coefficient and the illumination influence coefficient, combining a non-local mean filtering algorithm, and performing image enhancement on the wheat ear image; a disease and insect pest detection module: detecting diseases and insect pests of the wheat ear through the enhanced image; when denoising, the details of the disease and insect pest area are retained and effectively denoised, and the accuracy of subsequent detection of diseases and insect pests of the wheat ear in the field is improved.

This invention discloses the C783T SNP related to wheat ear grain number and its application. This SNP site corresponds to the 783rd base from the 5' end of the sequence shown in SEQ ID NO: 1, i.e., the C783T SNP site; the site is a C / T polymorphism. Based on this SNP site, this invention has developed reagents or kits for identifying or assisting in the identification of wheat ear grain number traits, and provides a method for identifying or assisting in the identification of wheat genotypes. Experimental verification using a large number of wheat materials revealed that wheat with a C / C homozygous genotype has a greater or candidate greater ear grain number than wheat with a T / T homozygous genotype. The SNP of this invention has high effectiveness and potential application value; by detecting this SNP, wheat with a higher ear grain number can be identified, which is of great value in the research or application of breeding high-yielding wheat varieties.

The present application relates to the technical field of plant breeding, and particularly relates to a wheat ear germination resistance related gene molecular marker combination and application thereof.The molecular marker combination comprises nucleotide sequences shown in SEQ ID NO.1-3;the nucleotide sequence shown in SEQ ID NO.1 has polymorphism at the 102th position, and the polymorphism is A / T;the nucleotide sequence shown in SEQ ID NO.2 has polymorphism at the 107th position, and the polymorphism is C / G;the nucleotide sequence shown in SEQ ID NO.3 has polymorphism at the 24th position, and the polymorphism is G / C.The present application provides a wheat ear germination resistance related gene molecular marker combination, which has high accuracy when applied to wheat ear germination resistance trait detection, and has important significance in the field of wheat breeding.

The application belongs to the field of molecular breeding, and particularly relates to a wheat ear sprouting resistance related CAPs molecular marker BM_phs2_CAPs and application thereof. A wheat ear sprouting resistance related core candidate gene is obtained from a wheat ear sprouting resistant variety Bimei No. 6, and is named as Taphs2-6A-BM. The gene sequence is significantly different among different haplotypes. Based on the variation, specific amplification primers are designed for a CDS1 region, and an efficient CAPs molecular marker for distinguishing ear sprouting resistance is developed by using ApaI enzyme cutting technology, and is named as BM_phs2_CAPs. The CAPs marker is applied to 296 white grain wheat varieties, and the result shows that the detection result of the BM_phs2_CAPs marker is highly consistent with the multi-year and multi-point field ear sprouting resistance phenotype data.

The invention discloses a KASP primer pair for identifying wheat ear types as well as an identification method and application of the KASP primer pair. The KASP primer pair comprises an upstream primer and a downstream primer, wherein the nucleotide sequences of the upstream primer 1 and the upstream primer 2 are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2; and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The KASP marker is a wheat ear type mutant gene functional molecular marker, and the SNP site corresponding to the KASP molecular marker is that G located at the 57941628th basic group of a wheat 1D chromosome is mutated into A. The technical scheme provided by the invention can be used for molecular marker-assisted selection and screening of wheat ear type new materials. The KASP primer pair provided by the invention can be used for simply, conveniently and quickly detecting and identifying the ear type of wheat at high throughput, and the ear type improvement of wheat can be accelerated through backcross selection.

The application relates to a molecular marker for identifying wheat ear length and spike density traits and application thereof, discloses a variation site related to wheat ear length and spike density traits, and develops an InDel marker through the site. KY5214 genotype or TaTPR-B1 W8762 genotype. Research finds that the ear length of a wheat plant with an excellent haplotype TaTPR-B1 W8762 genotype is less than that of a wheat plant with TaTPR-B1 KY5214 genotype, and the spike density is higher than that of a wheat plant with TaTPR-B1 KY5214 genotype. The finding helps to screen a wheat variety with small ear length and high spike density, provides a theoretical basis for cultivating a wheat variety with dense spikes and high yield, and provides a tool for molecular assisted selection.