16 results about "Wheatear" patented technology

The invention relates to the technical field of image processing, in particular to an intelligent detection system for field wheat ear diseases and insect pests. The system comprises an image acquisition module which is used for acquiring a field wheat ear image; the illumination analysis module is used for constructing an illumination influence coefficient based on a brightness value distribution condition in a neighborhood pixel block of each pixel point; the gray analysis module is used for determining a feature influence coefficient based on the gray value distribution condition in the neighborhood of each pixel point in the neighborhood pixel block; the image enhancement module is used for adaptively adjusting a preset basic attenuation parameter based on the feature influence coefficient and the illumination influence coefficient, and performing image enhancement on the wheat ear image in combination with a non-local mean filtering algorithm; the disease and insect pest detection module is used for detecting disease and insect pests of the wheat ears through the enhanced image; during denoising, detail reservation of the pest and disease damage area is enhanced, effective denoising is carried out, and the accuracy of subsequent field wheat ear pest and disease damage detection is improved.

The invention discloses application of protein TaTDM and related biological materials thereof, and belongs to the field of plant breeding. The amino acid sequence of the protein TaTDM is as shown in SEQ ID NO: 2 or SEQ ID NO: 4 or SEQ ID NO: 6. The TaTDM gene knockout homozygous mutant strains tatdmm1 and tatdmm2 are obtained by carrying out gene knockout on wild type wheat Fielder, and it is found that at the temperature of 25 DEG C, the total floret number of each spike of the Fielder, the tatdmm1 and the tatdmm2 and the survival rate under the water shortage treatment condition are remarkably higher than those of the wild type wheat Fielder. The protein TaTDM has an important application value in regulation and control of wheat ear type characters and stress tolerance, provides a high-quality germplasm resource, and is of great significance in cultivation of drought-enduring plant varieties and improvement of crop yield.

The invention discloses a wheat grain number related C783T SNP (single nucleotide polymorphism) and application thereof, the SNP site corresponds to the 783th basic group from the 5'tail end of the sequence shown as SEQ ID NO: 1, namely the C783T SNP site; the sites are C / T polymorphisms. The invention also develops a reagent or a kit for identifying or assisting in identifying the character of the number of wheat ears based on the SNP site, and provides a method for identifying or assisting in identifying the genotype of wheat. Through experimental verification of a large number of wheat materials, it is found that the grain number of wheat with the homozygous genotype C / C is larger than or candidate larger than the grain number of wheat with the homozygous genotype T / T. The SNP has high effectiveness and potential application value, wheat with high grain number per ear can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The application discloses a KASP molecular marker related to a wheat ear type and application, finds a key variation site related to the wheat ear type through first generation sequencing, and develops a corresponding KASP molecular marker, and the primer of the KASP molecular marker can be used for quickly and efficiently identifying whether the target sample has the variation site of the corresponding gene, screening or assisting in screening the wheat ear type phenotype in the early breeding stage, accelerating the genetic improvement process of the wheat ear type, greatly improving the selection efficiency and quality of the wheat variety or strain, and having important value in the research or application of cultivating a high-yield wheat variety.

The application relates to the technical field of image processing, in particular to an intelligent detection system for wheat ear diseases and insect pests in a field. The system comprises: an image acquisition module: acquiring a wheat ear image in a field; an illumination analysis module: constructing an illumination influence coefficient of each pixel point based on the brightness value distribution in the neighborhood pixel block of the pixel point; a gray scale analysis module: determining a feature influence coefficient of each pixel point in the neighborhood pixel block based on the gray scale value distribution in the neighborhood of the pixel point; an image enhancement module: adaptively adjusting a preset basic attenuation parameter based on the feature influence coefficient and the illumination influence coefficient, combining a non-local mean filtering algorithm, and performing image enhancement on the wheat ear image; a disease and insect pest detection module: detecting diseases and insect pests of the wheat ear through the enhanced image; when denoising, the details of the disease and insect pest area are retained and effectively denoised, and the accuracy of subsequent detection of diseases and insect pests of the wheat ear in the field is improved.

The invention belongs to the technical field, and discloses a wheat breeding pollination method and device, the wheat breeding pollination device comprises a fixed plate, a pollination assembly and a storage assembly, the top of the fixed plate is provided with a moving track, the outer wall of the moving track is slidably provided with the pollination assembly, and the pollination assembly comprises a moving box arranged on the outer wall of the moving track; connecting plates are mounted on the outer walls of the two sides of the moving box, a pollination groove is formed in the bottom of the moving box, and a motor is mounted on the outer wall of the pollination groove. By arranging the pollination cover, wheat can be wrapped, pollination can be carried out while wrapping is carried out, pollen waste can be reduced, the pollination rate can be increased, and the two sides of wheat ears are wrapped, so that pollen can be effectively prevented from being blown away by wind during pollination, the pollination range of the pollen can be controlled, waste is reduced, and the pollination efficiency is improved. The pollen is sprayed out through the plurality of discharging holes, so that the pollen can cover all positions of wheat ears, the pollination rate of wheat is increased, and the pollination effect is improved.

The present application relates to the technical field of biology, and specifically provides a molecular marker for detecting wheat ear bud resistance and application. Genotype data is obtained by using Wheat55K wheat high-throughput gene chip, a QTL site Qphs.caas-3DS related to ear bud resistance is detected from a resistant material, and a KASP marker and a detection primer set thereof are developed to efficiently screen high and low ear bud resistance. The wheat genomic DNA is subjected to PCR amplification by using the primer set of the present application, and whether the ear bud resistance gene is carried can be directly determined by KASP typing, the detection method is simple to operate, the detection result is very intuitive, the detection effect is obvious and effective, and the use of the molecular marker for screening can greatly improve the efficiency of molecular marker assisted selection of wheat breeding with ear bud resistance.

The invention discloses a KASP molecular marker related to wheatear type and application, a key variation site related to wheatear type is found through first-generation sequencing, the polymorphism of the key variation site is A or T, and the corresponding KASP molecular marker is developed. The primer of the KASP molecular marker can be used for quickly and efficiently identifying whether a target sample has a variation site of a corresponding gene or not, screening or auxiliary screening is carried out on wheat panicle type phenotypes in the early breeding stage, the genetic improvement process of the wheat panicle types can be accelerated, the selection efficiency and quality of wheat varieties or strains are greatly improved, and the application prospect is wide. The method has important value in research or application of cultivation of high-yield wheat varieties.

The invention belongs to the technical field of wheat molecular genetic breeding, and discloses a QTL (quantitative trait loci) location influencing wheat ear length and a candidate gene and application thereof, the QTL location influencing the wheat ear length comprises eight wheat ear length QTL loci which are respectively QSl.wa-2D, QSl.wa-4A. 1, QSl.wa-4A. 2, QSl.wa-4D. 1, QSl.wa-4D. 2, QSl.wa-5D, QSl.wa-7Da and QSl.wa-7Db. The new QTL site and the candidate gene of the wheat ear length are found, and theoretical and technical support is provided for molecular marker-assisted breeding.

This invention discloses the C783T SNP related to wheat ear grain number and its application. This SNP site corresponds to the 783rd base from the 5' end of the sequence shown in SEQ ID NO: 1, i.e., the C783T SNP site; the site is a C / T polymorphism. Based on this SNP site, this invention has developed reagents or kits for identifying or assisting in the identification of wheat ear grain number traits, and provides a method for identifying or assisting in the identification of wheat genotypes. Experimental verification using a large number of wheat materials revealed that wheat with a C / C homozygous genotype has a greater or candidate greater ear grain number than wheat with a T / T homozygous genotype. The SNP of this invention has high effectiveness and potential application value; by detecting this SNP, wheat with a higher ear grain number can be identified, which is of great value in the research or application of breeding high-yielding wheat varieties.

The invention discloses a wheat ear length related molecular marker and application thereof. The invention provides an application of a substance for detecting the genotype of an SNP (Single Nucleotide Polymorphism) A site and the genotype of an SNP B site in a wheat genome or a substance for detecting haplotype in the wheat genome in any one of the following aspects: identifying or assisting in identifying the ear length character of wheat; the genotype of the SNP A site is AA or TT; the genotype of the SNP B site is AA or GG; the haplotype is a combination of the genotype A of the SNP site and the genotype B of the SNP site in a wheat genome; two CAPS markers are developed on the basis that two SNP variation sites exist in CDS of the TaSIK1-5A gene in a natural wheat population, and the two CAPS markers are used for identifying TaSIK1-5A-Hap1 and TaSIK1-5A-Hap2 genotypes and evaluating the ear length of a material.

The present application relates to the technical field of plant breeding, and particularly relates to a wheat ear germination resistance related gene molecular marker combination and application thereof.The molecular marker combination comprises nucleotide sequences shown in SEQ ID NO.1-3;the nucleotide sequence shown in SEQ ID NO.1 has polymorphism at the 102th position, and the polymorphism is A / T;the nucleotide sequence shown in SEQ ID NO.2 has polymorphism at the 107th position, and the polymorphism is C / G;the nucleotide sequence shown in SEQ ID NO.3 has polymorphism at the 24th position, and the polymorphism is G / C.The present application provides a wheat ear germination resistance related gene molecular marker combination, which has high accuracy when applied to wheat ear germination resistance trait detection, and has important significance in the field of wheat breeding.

The application belongs to the field of molecular breeding, and particularly relates to a wheat ear sprouting resistance related CAPs molecular marker BM_phs2_CAPs and application thereof. A wheat ear sprouting resistance related core candidate gene is obtained from a wheat ear sprouting resistant variety Bimei No. 6, and is named as Taphs2-6A-BM. The gene sequence is significantly different among different haplotypes. Based on the variation, specific amplification primers are designed for a CDS1 region, and an efficient CAPs molecular marker for distinguishing ear sprouting resistance is developed by using ApaI enzyme cutting technology, and is named as BM_phs2_CAPs. The CAPs marker is applied to 296 white grain wheat varieties, and the result shows that the detection result of the BM_phs2_CAPs marker is highly consistent with the multi-year and multi-point field ear sprouting resistance phenotype data.

The invention discloses a KASP primer pair for identifying wheat ear types as well as an identification method and application of the KASP primer pair. The KASP primer pair comprises an upstream primer and a downstream primer, wherein the nucleotide sequences of the upstream primer 1 and the upstream primer 2 are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2; and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The KASP marker is a wheat ear type mutant gene functional molecular marker, and the SNP site corresponding to the KASP molecular marker is that G located at the 57941628th basic group of a wheat 1D chromosome is mutated into A. The technical scheme provided by the invention can be used for molecular marker-assisted selection and screening of wheat ear type new materials. The KASP primer pair provided by the invention can be used for simply, conveniently and quickly detecting and identifying the ear type of wheat at high throughput, and the ear type improvement of wheat can be accelerated through backcross selection.

The present invention discloses a KASP marker related to the length of the internode below the wheat ear and its application in assisted breeding, belonging to the technical field of crop seed selection and cultivation. Among them, the KASP marker primer set FSI-6B includes the upstream primer F1 shown in SEQ ID NO:1, the upstream primer F2 shown in SEQ ID NO:2, and the downstream primer R shown in SEQ ID NO:3. The KASP marker primer set FSI-6B provided by the present invention can be used to detect the variation of base A or G at the position of 610799456 bp on chromosome 6B of wheat. The variation of base A or G at this position is significantly related to the internode below the wheat ear. When the genotype at this position in the wheat genome is GG, the wheat shows a long internode below the ear; when the genotype is AA, the wheat shows a short internode below the ear; when the genotype is AG, the length of the internode below the wheat ear is between the two.

The application relates to a molecular marker for identifying wheat ear length and spike density traits and application thereof, discloses a variation site related to wheat ear length and spike density traits, and develops an InDel marker through the site. KY5214 genotype or TaTPR-B1 W8762 genotype. Research finds that the ear length of a wheat plant with an excellent haplotype TaTPR-B1 W8762 genotype is less than that of a wheat plant with TaTPR-B1 KY5214 genotype, and the spike density is higher than that of a wheat plant with TaTPR-B1 KY5214 genotype. The finding helps to screen a wheat variety with small ear length and high spike density, provides a theoretical basis for cultivating a wheat variety with dense spikes and high yield, and provides a tool for molecular assisted selection.