Method for breeding lean-type Chinese Huai pigs in multi-gene pyramiding manner based on growth traits thereof

Abstract

The invention relates to a method for breeding lean-type Chinese Huai pigs in a multi-gene pyramiding manner based on growth traits thereof. The method comprises the following operating steps: 1, carrying out DNA (deoxyribonucleic acid) extraction on the genome of the pig; 2, designing primers, more particularly, designing the PCR (polymerase chain reaction) amplification primers according to the gene sequences of insulin-like growth factor-I (IGF-I), pituitary specific transcription factor-I (PIT-I), liver X receptor alpha (LXR alpha) and melanocortin-4 receptor (MC4R); 3, carrying out the PCR; 4, carrying out restriction fragment length polymorphism (RFLP); and 5, carrying out the correlation analysis on the polymorphism and growth traits of genes. According to the analysis, the method can determine that the single growth rate of the pyramided gene with the gene type thereof being AADDGGFF is the highest one; the method can prevent the genes of other offsprings from being isolated after the selective reservation for breeding, thus achieving the optimal effect of the growth traits of the offsprings on the four gene types; and the method can stabilize the inheritance, particularly lead the genes of the growth traits to become homozygous within one generation, thus accelerating the cultivation of the lean-type Chinese Huai pigs.

Description

technical field [0001] The invention belongs to the technical field of animal breeding, and in particular relates to a breeding method for a lean Huai pig strain. Background technique [0002] The disadvantages of traditional pig breeding methods and single-gene marker-assisted selection are as follows: (1) In pig breeding, individual genetic evaluation is based on phenotypic information and pedigree information, although traditional breeding methods provide us with the ability to use this information for It is the best means to estimate the breeding value, but in some cases, the selection based on the traditional breeding method still cannot achieve the desired effect, such as low heritability traits and threshold traits, due to the genetic information contained in the phenotypic information Very limited, unless there is a large amount of information about various relatives, it is difficult to make an accurate genetic assessment of the individual; as for the limited traits,...

AI Technical Summary

Problems solved by technology

[0002] The disadvantages of traditional pig breeding methods and single-gene marker-assisted selection are as follows: (1) In pig breeding, individual genetic evaluation is based on phenotypic information and pedigree information, although traditional breeding methods provide us with the ability to use this information for It is the best means to estimate the breeding value, but in some cases, the selection based on the traditional breeding method still cannot achieve the desired effect, such as low heritability traits and threshold traits, due to the genetic information contained in the phenotypic information Very limited, unless there is a large amount of information about various relatives, it is difficult to make an accurate genetic assessment of the individual; as for the limited traits, individuals who cannot express traits can only be evaluated based on the performance of their siblings and descendants. If only sibling information is used, the accuracy of assessment is generally low due to the limited number of siblings; if descendant information is used, and the number of descendant information is large, the assessment accuracy can be very high, but the generation interval is prolonged and the genetic progress is relatively low

(2) Low efficiency of single gene selection

Yu Peichu et al. (2004) believed that the two mutations in Xiang pigs may cause a decrease in the expression of pituitary-specific transcription factor-I (PIT-I), which may eventually affect the growth of Xiang pigs.

It can be seen that most of the pituitary-specific transcription factor-I (PIT-I) genes are polymorphic in different breeds of pigs, but the gene effects are not consistent

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