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SNP marker related to tail type trait of fat tail type sheep, and applications thereof
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A sheep and marker technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of prone to parasites, frostbite, anus, vulva exposure, etc., to promote the breeding process, save money time, low cost
Active Publication Date: 2018-08-10
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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In addition, in the cold northern regions, according to the artificial tail docking method, the tail is completely removed, which will lead to the exposure of the anus and vulva, resulting in chilblains, and easy parasites in summer.
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Embodiment 1
[0032] Example 1: Determination of a SNP marker related to the tail shape traits of fat-tailed sheep and its detection method and application
[0034] Randomly collect EDTA anticoagulant blood samples from 10 sheep in Ujimqin sheep, Sunit sheep, Barhu sheep, and Hulunbeier short-tailed sheep, and use a conventional blood genome extraction kit to extract genomic DNA from the blood;
[0035] 2. Specific primer sequence
[0036] According to the sequence of the T gene locus included in the NCBI database, primers for the screened T gene SNP site were designed, and the primer sequence was: forward primer (sequence listing sequence 2): 5'-CACAAGAAGGTGCAGAGTCACA-3'; reverse primer (sequence Listed sequence 3): 5'-CCTGCCTTTGGCCTCACGAT-3'.
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Abstract
The invention relates to a SNP marker related to the tail type trait of fat tail type sheep, and applications thereof, and belongs to the biological field, wherein the SNP marker is located at the 87804589-th base pair of sheep chromosome 8, the sheep with the base of T at the locus has the short tail trait, and the sheep with the base of G has the normal tail length. The invention further provides primers for detecting the SNP marker, a kit containing the primers, and applications of the SNP marker in identification of the tail type trait of lipid tail type sheep and in breeding of Chinese sheep, wherein the SNP marker related to the tail type trait of fat tail type sheep is screened by using a polymerasechain reaction (PCR) and a sequencing technology, and the genotype of to-be-detectedsheep is detected through a restriction fragment length polymorphism (RFLP) method. According to the present invention, with the SNP marker, the breeding of the dominant species with the tail type trait of fat tail type sheep is performed according to the genotype so as to accelerate the breeding process of the fat tail type sheep.
Description
technical field [0001] The invention relates to a SNP marker related to the tail shape traits of fat-tailed sheep and its application. Background technique [0002] As people's demand for mutton rises, sheep farming has already become a very important part of animal husbandry production. In the sheep raising industry in Inner Mongolia and even my country, an important problem is the fat deposition of sheep tail. About 25% of the sheep breeds in the world belong to the fat tail (rump) type (fat tail / fat rump) sheep breeds. This kind of breed has a super fat accumulation ability in the tail, and some breeds such as Daweihan sheep tail fat content even exceed 25% of the total carcass weight. Before the reform and opening up, sheep tail fat was eaten as edible oil. But now sheep tail fat is rarely used for food, and high-fat meat is gradually neglected. From the perspective of feeding costs and benefits, sheep tails with large fat tails are also used. It is difficult to promo...
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