SNP marker related to tail type trait of fat tail type sheep, and applications thereof

A sheep and marker technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of prone to parasites, frostbite, anus, vulva exposure, etc., to promote the breeding process, save money time, low cost

Active Publication Date: 2018-08-10
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the cold northern regions, according to the artificial tail docking method, the tail is completel...

Method used

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  • SNP marker related to tail type trait of fat tail type sheep, and applications thereof
  • SNP marker related to tail type trait of fat tail type sheep, and applications thereof
  • SNP marker related to tail type trait of fat tail type sheep, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Determination of a SNP marker related to the tail shape traits of fat-tailed sheep and its detection method and application

[0033] 1. Sample collection and DNA extraction

[0034] Randomly collect EDTA anticoagulant blood samples from 10 sheep in Ujimqin sheep, Sunit sheep, Barhu sheep, and Hulunbeier short-tailed sheep, and use a conventional blood genome extraction kit to extract genomic DNA from the blood;

[0035] 2. Specific primer sequence

[0036] According to the sequence of the T gene locus included in the NCBI database, primers for the screened T gene SNP site were designed, and the primer sequence was: forward primer (sequence listing sequence 2): 5'-CACAAGAAGGTGCAGAGTCACA-3'; reverse primer (sequence Listed sequence 3): 5'-CCTGCCTTTGGCCTCACGAT-3'.

[0037] 3. Amplify the target fragment in vitro

[0038] (1) Prepare 50μl PCR reaction system:

[0039] 50 ng / μl template DNA 4μl,

[0040] 10pmol / μl forward primer and reverse primer each 2μl, ...

Embodiment 2

[0059] Example 2: Kit and application for identifying tail-type traits of fat-tailed sheep

[0060] The components in the kit are as follows:

[0061] 10pmol / μl upstream primer 100μl

[0062] 10pmol / μl downstream primer 100μl

[0063] 2×Taq PCR MasterMix 1.25ml

[0064] Pas I endonuclease 50μl

[0065] 10×Buffer 100μl

[0066] Deionized water 2ml,

[0067] Total PCR reaction system in the kit:

[0068] Make up to 50μl with deionized water

[0069] 2×Taq PCR MasterMix 25μl

[0070] Template DNA 200ng

[0071] 10pmol / μl upstream primer 2μl

[0072] 10pmol / μl downstream primer 2μl;

[0073] PCR reaction program:

[0074] 94°C, 5 minutes; 94°C, 30 seconds; 57°C, 30 seconds; 72°C, 30 seconds; 35 cycles; 72°C, 10 minutes, 4°C storage.

[0075] Pas I digestion reaction system in the kit:

[0076] PasI endonuclease 1μl

[0077] 10×Buffer 2μl

[0078] PCR product 5μl

[0079] 12 μl of deionized water.

[0080] Pas I reaction procedure:

[0081] Reaction at 55°C for 6h...

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PUM

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Abstract

The invention relates to a SNP marker related to the tail type trait of fat tail type sheep, and applications thereof, and belongs to the biological field, wherein the SNP marker is located at the 87804589-th base pair of sheep chromosome 8, the sheep with the base of T at the locus has the short tail trait, and the sheep with the base of G has the normal tail length. The invention further provides primers for detecting the SNP marker, a kit containing the primers, and applications of the SNP marker in identification of the tail type trait of lipid tail type sheep and in breeding of Chinese sheep, wherein the SNP marker related to the tail type trait of fat tail type sheep is screened by using a polymerase chain reaction (PCR) and a sequencing technology, and the genotype of to-be-detectedsheep is detected through a restriction fragment length polymorphism (RFLP) method. According to the present invention, with the SNP marker, the breeding of the dominant species with the tail type trait of fat tail type sheep is performed according to the genotype so as to accelerate the breeding process of the fat tail type sheep.

Description

technical field [0001] The invention relates to a SNP marker related to the tail shape traits of fat-tailed sheep and its application. Background technique [0002] As people's demand for mutton rises, sheep farming has already become a very important part of animal husbandry production. In the sheep raising industry in Inner Mongolia and even my country, an important problem is the fat deposition of sheep tail. About 25% of the sheep breeds in the world belong to the fat tail (rump) type (fat tail / fat rump) sheep breeds. This kind of breed has a super fat accumulation ability in the tail, and some breeds such as Daweihan sheep tail fat content even exceed 25% of the total carcass weight. Before the reform and opening up, sheep tail fat was eaten as edible oil. But now sheep tail fat is rarely used for food, and high-fat meat is gradually neglected. From the perspective of feeding costs and benefits, sheep tails with large fat tails are also used. It is difficult to promo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 曹贵方智达夫刘默凝王鑫龙鑫
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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