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55 results about "Duck circovirus" patented technology
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The Duck circovirus (DuCV) is a type of virus found in ducks. Strains of the virus have predominantly been found in China, though strains have also been isolated from ducks in Germany and the United States.
GeXP (Gene Expression Profiler) detection kit for differentiating 11 kinds of duck viral diseases
ActiveCN103773899AStrong specificityImprove throughputMicrobiological testing/measurementDNA/RNA fragmentationDiseaseDuck hepatitis A virus
Owner:GUANGXI VETERINARY RES INST
Real-time fluorescence quantification PCR detection primer for quickly identifying genotypes of duck circovirus and detection method thereof
PendingCN106065419AIdentification method is simpleImprove shortcutMicrobiological testing/measurementMicroorganism based processesFluorescencePcr method
The invention relates to a real-time fluorescence quantification PCR detection primer for quickly identifying genotypes of duck circovirus and a detection method thereof. The sequences of the primer are described as follows: an upstream primer P1: 5'-CAGATCCCCGGGCACGAGA-3'; a downstream primer P2: 5'-CCTCACCTTCAGGGATTC-3'. The detection method includes the steps of: establishing the real-time fluorescence quantification PCR method based on SYBR Green I with the primer, wherein difference of temperature of a melting curve exists due to the difference of GC content of a nucleotide in a specific gene fragment region of gene 1-type duck circovirus and gene 2-type duck circovirus which are amplified by the primer, so that infection of the gene 1-type duck circovirus and the gene 2-type duck circovirus can be directly carried out according to the difference of Tm value in the melting curve. The method can distinguish the infection caused by the duck circovirus in different genotypes just with one group of primer, thereby providing technical fundament for healthy breeding in duck breeding industry.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
PCR-RFLP primer and method for rapidly identifying genotypes of duck circoviruses
InactiveCN105177186AIdentification method is simpleMicrobiological testing/measurementMicroorganism based processesRestriction Enzyme Cut SiteDistinctin
The invention discloses a PCR-RFLP primer and method for rapidly identifying the genotypes of duck circoviruses. The primer comprises an upstream primer body P1:5'-TGCGCCAAAGAGTCGACATAC-3' and a downstream primer body P2:5'-CAAATTGGTCTCAGTAGTTTATT-3'. According to the method, distinguishing is carried out through the difference of restriction enzyme cutting sites contained in coding regions of copied protein genes of the duck circoviruses in different genotypes (the first genotype and the second genotype). The method includes the steps that DNA is extracted, Kpn I restriction enzyme cutting is carried out after PCR amplification is carried out to obtain corresponding target fragments, and then RFLP analysis is carried out. The identification method is rapid and easy to operate and high in accuracy.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
GeXP Detection Kits for Identification of 11 Kinds of Duck Virus Diseases
ActiveUS20160060716A1Strong specificityNucleotide librariesMicrobiological testing/measurementDuck hepatitis A virusDisease
Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The can kit detect, simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.
Owner:GUANGXI VETERINARY RES INST
Duplex polymerase chain reaction (PCR) method for rapidly identifying duck circovirus genotype
InactiveCN104059998AIncreased sensitivityAccurate identificationMicrobiological testing/measurementDNA/RNA fragmentationDuplex pcrGenotype
The invention provides a duplex polymerase chain reaction (PCR) method for rapidly identifying duck circovirus genotype. The method comprises the following steps: comparing sequences DuCV-1 and DuCV-2 released on reference GenBank, selectively and respectively designing a pair of general specific primers SEQ1 / SEQ2 aiming at DuCV, a type 1 duck circovirus specific primer SEQ3 and a type 2 duck circovirus specific primer SEQ4 in a conserved region of the DuCV-1 and DuCV-2. According to the method, novel specific primers are designed and screened, various parameters in the reaction process are optimized, the dual PCR sequence-based typing method aiming at the duck circovirus established by utilizing the primers is high in specificity and high in sensitivity, and the sequences DuCV-1 and DuCV-2 can be rapidly and accurately identified.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Triple polymerase chain reaction (PCR) kit for duck hepatitis virus type I, duck circoviruses and Muscovy duckling parvovirosis and application of triple PCR kit
ActiveCN102719564AStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesDuck hepatitis A virusSingle strand
The invention discloses a primer pair group and a kit for performing identification or auxiliary identification on duck-related viruses and application of the primer pair group and the kit. The primer pair group consists of three primer pairs, and the three primer pairs consist of two single-stranded deoxyribonucleic acids (DNA) which are shown as a sequence 1 and a sequence 2, a sequence 3 and asequence 4, and a sequence 5 and a sequence 6 respectively; and the duck-related viruses are at least one of Muscovy duckling parvovirosis, duck circoviruses and duck hepatitis virus type I. A triplepolymerase chain reaction (PCR) technology that three kinds of pathogens, namely the Muscovy duckling parvovirosis, the duck circoviruses and the duck hepatitis virus type I can be simultaneously detected and identified through one-time PCR is established, and has the advantages of high specificity and sensitivity (the lowest detection line is 1pg), low cost, high efficiency and the like; and moreover, an amplification result is directly determined by utilizing the difference of the length of amplified fragments in the aspect of primer design, so that the method is relatively simple, convenient, intuitive and practical during result determination.
Owner:GUANGXI VETERINARY RES INST
Duplex PCR (polymerase chain reaction) detection kit for duck Newcastle disease virus and duck circovirus
ActiveCN102618668ASimple and fast operationIncreased sensitivityMicrobiological testing/measurementDNA/RNA fragmentationDuplex pcrNewcastle disease virus NDV
The invention discloses a duplex PCR (polymerase chain reaction) detection kit for duck Newcastle disease virus and duck circovirus, and particularly relates to a primer group for detecting Newcastle disease virus and duck circovirus, which comprises a first primer, a second primer, a third primer and a fourth primer. Nucleotide sequences of the first primer, the second primer, the third primer and the fourth primer are respectively a first sequence, a second sequence, a third sequence and a fourth sequence in a sequence list. Testing shows that the two pairs of primers are designed to establish a duplex PCR detection method for Newcastle disease virus and duck circovirus, and the duplex PCR technique capable of detecting two pathogens, namely the Newcastle disease virus and the duck circovirus has the advantages of simplicity in operation, high sensitivity, high specificity, high repeatability and the like.
Owner:GUANGXI VETERINARY RES INST
Dual-PCR (Polymerase Chain Reaction) assay kit for duck circovirus and duck hepatitis virus
ActiveCN102605104AStrong specificityThe result is easy to judgeMicrobiological testing/measurementDNA/RNA fragmentationDuck hepatitis A virusPcr assay
The invention discloses a dual-PCR assay kit for duck circovirus and duck hepatitis virus. The dual-PCR assay kit provides a primer group for assaying duck circovirus and duck hepatitis virus, which consists of a primer 1, a primer 2, a primer 3 and a primer 4; and the nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 sequentially in a sequence table. The dual-PCR technique which can simultaneously assay and identify DuCV (duck circovirus) and DHV (duck hepatitis virus) as two pathogens by establishing PCR reaction just once has good specificity, moreover, the experiment utilizes the difference of amplified fragment lengths to directly determine an amplification result in the primer design, and thereby the method is simpler, more visual and more practical during result determination.
Owner:GUANGXI VETERINARY RES INST
PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method for differentiating duck circovirus from goose circovirus
ActiveCN103602759AIdentification method is simpleImprove efficiencyMicrobiological testing/measurementMicroorganism based processesDistinctinRestriction fragment length polymorphism
The invention discloses a PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method for differentiating duck circovirus from goose circovirus. The PCR-RFLP method is used for differentiating by utilizing Rep protein gene sequence enzyme cutting site difference and comprises the following steps of: extracting DNA (Deoxyribonucleic Acid), carrying out PCR amplification to obtain a Rep protein gene segment, and carrying out RFLP analysis after carrying out XhoI digestion. The PCR-RFLP method disclosed by the invention is simple and higher in efficiency and accuracy.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Duck circovirus genetic engineering subunit vaccine and preparation method and application thereof
InactiveCN110237243AOutstanding advantagesOutstanding effectViral antigen ingredientsVirus peptidesNucleotideOuter capsid
The invention provides a duck circovirus genetic engineering subunit vaccine and a preparation method and application thereof. A nucleic acid molecule of which the sequence is as shown in SEQID NO:1 or a nucleic acid molecule of which the nucleotide sequence is 95% or above same as that as shown in the SEQID NO:1 is used for coding duck circovirus outer capsid structural protein, an immune composition containing the duck circovirus outer capsid structural protein can be used for preparing the duck circovirus genetic engineering subunit vaccine, the antigenicity, the immunogenicity and the function of the vaccine are similar to those of natural protein, and the vaccine is high in expression level, high in immunogenicity and free from pathogenicity to ducks. The vaccine can be in large-scale serum-free suspension culture and preparation through a bioreactor, and the production cost of the vaccine can be greatly reduced.
Owner:苏州世诺生物技术有限公司
Indirect ELISA method for detecting duck circovirus antibody
InactiveCN101806800AImprove featuresGood repeatabilityVirus peptidesFermentationProkaryotic expressionElisa method
The invention relates to an indirect ELISA method for detecting a duck circovirus (DuCV) antibody. The method uses a pET-32a(+) protokaryon to express the carrier, constructs a gene engineering bacterium BLpET-32a-Cap which can express the partial capsid protein (Cap) of the DuCV, and coats an ELISA plate with the expressed recombinant protein which is subject to purification and renaturation to detect the antibody level of DuCV in the duck blood serum. The result shows that the method has the characteristics of good repeatability and high specificity, and can be used for serological research on the DuCV.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Duck circovirus and adenovirus bivalent inactivated vaccine and preparation method of yolk antibody thereof
InactiveCN111420042ASolving Manufacturing ChallengesBest ratioEgg immunoglobulinsViral antigen ingredientsAntigenAdjuvant
The invention relates to a duck circovirus and adenovirus bivalent inactivated vaccine and a preparation method of a yolk antibody thereof. A duck circovirus antigen is obtained by infecting 1-day-oldhealthy cherry valley ducklings and collecting infected duck livers at the age of 25 days to obtain antigen tissues, and an adenovirus antigen is obtained by infecting SPF chickens of 20 days old to30 days old and collecting livers of dead chickens; an antigen of the vaccine consists of liver tissues infected with the duck circovirus and liver tissues infected with the adenovirus in a mass ratioof (1-5):1, and an antigen solution for the vaccine is prepared; and a pine pollen polysaccharide with a concentration of 5-40mg / mL is added into the antigen solution to serve as an immunopotentiator, and the antigen solution is emulsified with a conventional white oil adjuvant to obtain the vaccine. The yolk antibody is obtained by immunizing laying hens with the vaccine, and then performing extracting and purifying on egg yolks of high-immunity laying hens, and the yolk antibody simultaneously contains two antibodies against the duck circovirus and the adenovirus. The vaccine and yolk antibody provided by the invention have the advantages of simple preparation process, low cost, good action effect, stable dosage form and easy storage, and has a wide application prospect.
Owner:山东百瑞凯来生物科技有限公司
Primer and probe for real-time fluorescence quantitative PCR detection of two genotypes of duck circovirus
ActiveCN111676328AQuick checkEfficient detectionMicrobiological testing/measurementMicroorganism based processesGenotypeFluorescent reporter
The invention relates to a primer and probe for real-time fluorescence quantitative PCR detection of two genotypes of duck circovirus. Sequences of the primer and the probe are as follows: an upstreamprimer DuCV-F: 5'-CAGTTTGTKGCTAARACVTTG-3'; a downstream primer DuCV-R: 5'-AGTTTATTGGRAASGGGAGG-3'; a probe DuCV-1-pobe: 5'-AGAGCGAGTTTGACCTYT-3'; a probe DuCV-2-pobe: 5'-TTTGATTTGTCCGCCTTAT-3', wherein the 5'- end of the DuCV-1-pobe is labelled with a fluorescent reporter group Cy5; and the 5'- end of the DuCV-2-pobe is labelled with a fluorescent reporter group FAM. The method is high in sensitivity and specificity, and can be used for genotype identification of two kinds of duck circovirus.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Primer probe combination and kit for simultaneously detecting duck circovirus, duck plague virus and duck adenovirus 3
ActiveCN112063760AGuaranteed accuracyQuick checkMicrobiological testing/measurementDNA/RNA fragmentationPoultry diseaseNucleotide
The invention provides a primer probe combination for simultaneously detecting duck circovirus, duck plague virus and duck adenovirus 3, and belongs to the technical field of livestock and poultry disease diagnosis. The primer probe combination comprises a primer pair and a probe, a nucleotide sequence of an upstream primer of the primer pair is shown as SEQ ID No.1, and a nucleotide sequence of adownstream primer of the primer pair is shown as SEQ ID No.2; the probe comprises a duck circovirus probe, a duck plague virus probe and a duck adenovirus 3 probe, a nucleotide sequence of the duck circovirus probe is shown as SEQ ID No.3, a nucleotide sequence of the duck plague virus probe is shown as SEQ ID No.4, and a nucleotide sequence of the duck adenovirus 3 probe is shown as SEQ ID No.5.By adopting the primer probe combination provided by the invention, the duck circovirus, the duck plague virus and the duck adenovirus 3 can be simultaneously detected.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Duck reovirus and duck circovirus bivalent inactivated vaccine and preparation method thereof
PendingCN113384692AHigh titer contentAvoid adverse reactionsViral antigen ingredientsAntiviralsReovirus (antigen)Leb antigen
The invention discloses a duck reovirus and duck circovirus bivalent inactivated vaccine and a preparation method thereof. The bivalent inactivated vaccine comprises an effective amount of inactivated antigen and an immunologic adjuvant in immunization, wherein the inactivated antigen comprises duck reovirus antigen and duck circovirus antigen proteins. The invention further discloses a preparation method of the bivalent inactivated vaccine. The bivalent inactivated vaccine provided by the invention is good in safety, does not generate mutual interference or influence of antigen components, can achieve the protection effect of secondary immunization of each single vaccine through one-time immunization, can prevent duck reovirus and duck circovirus at the same time, and can avoid adverse reaction caused by multiple times of immunization, and the preparation method has the advantages of simplicity, convenience, multiple effects, low cost, high vaccine titer content and the like.
Owner:哈药集团生物疫苗有限公司
Fluorescent quantitative primer group for visual differential diagnosis of waterfowl circoviruses
ActiveCN103882152AIdentification method is simpleImprove efficiencyMicrobiological testing/measurementMicroorganism based processesBiotechnologyNucleotide
The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer group for visual detection on infection conditions of duck circovirus (DuCV) and goose circovirus (GoCV) and a method thereof. According to the method, the detection on the infection conditions of DuCV and GoCV is carried out by using dissolution curve temperature Tm value difference caused by the difference between nucleotide GC contents of DuCV and GoCV specific gene fragment regions amplified by primers, and the infection conditions of DuCV and GoCV can be subjected to visual differential diagnosis specifically by only combining the SYBR Green I based real-time fluorescent quantitative PCR primer group to difference of dissolution curve Tm values which are automatically generated after reaction is ended. The method disclosed by the invention is simple and is relatively high in efficiency and accuracy.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
In vitro proliferation and culture method for duck circovirus
ActiveCN108841795AHigh titerAchieve separation and identificationBlood/immune system cellsSsDNA virusesDiseaseIn vitro proliferation
The invention provides an in vitro proliferation and culture method for a duck circovirus. The method comprises the following steps: (1) collecting healthy adult Muscovy duck blood for isolated culture of PBMC to prepare a PBMC cell suspension; and (2) adding a disease material which is detected duck circovirus positive into the PBMC cell suspension to be cultured. The invention provides the method of culturing DuCV by in vitro proliferation. The virus titer after virus inoculating culture is improved greatly, isolation and identification of the virus can be finished in a lab, and relatively good help is provided for researching the nosogenesis and damage of the virus.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Triple inactivated vaccine for duck circovirus disease, novel duck reovirus disease and duck virus hepatitis and preparation method of triple inactivated vaccine
PendingCN113491767AHigh titer contentAvoid adverse reactionsSsRNA viruses positive-senseViral antigen ingredientsDuck hepatitis A virusDisease
Owner:哈药集团生物疫苗有限公司
Duck circovirus ORF3 nucleus location NLS sequence and application thereof
The invention discloses a nuclear localization signal polypeptide, the sequence of which is RRLRTCNCRACRTLKH, and the double repetition of the signal polypeptide sequence can increase the expression of the fusion protein in eukaryotic cells.
Owner:LINYI UNIVERSITY +1
Modified duck circovirus Cap protein and preparation method and application thereof
ActiveCN111454336APromote recombinant expressioPromotes proper foldingEgg immunoglobulinsViral antigen ingredientsEscherichia coliArginine
The invention aims to provide a modified duck circovirus Cap protein as well as a preparation method and application thereof, that is, 21-36 peptide fragments rich in arginine at the N-terminal of theduck circovirus Cap protein are deleted, and a universal T cell epitope is introduced into a deletion region to promote immunogenicity; the amino acid sequence of the obtained modified duck circovirus Cap protein is SEQ ID NO: 1, and one nucleotide sequence is SEQ ID NO: 2. The modified duck circovirus Cap protein gene is connected with an expression vector and then transferred into escherichia coli BL21 (DE3), efficient soluble expression of duck circovirus Cap protein is achieved, the expressed protein is spontaneously assembled into virus-like particles, and the particles can be used for development of products such as duck circovirus gene engineering subunit vaccine and yolk antibody.
Owner:WEIFANG HUAYING BIOTECH CO LTD
Primers and probe for dual real-time fluorescent quantitative PCR detection of duck circovirus type 1 and duck circovirus type 2
PendingCN111748652AQuick checkEfficient detectionMicrobiological testing/measurementDNA/RNA fragmentationBioinformaticsDuck circovirus
The invention relates to a primer and a probe for dual real-time fluorescent quantitative PCR detection of duck circovirus type 1 and duck circovirus type 2. The sequences of the primer and the probeare as shown in SEQ. ID. No.1 to SEQ.ID. No.6. A dual real-time fluorescent quantitative PCR detection method established by utilizing the primers and the probe is high in sensitivity, good in stability, strong in specificity and good in repeatability, can be used for duck circovirus typing detection, and lays a foundation for subsequent scientific research on genotype pathogenesis of two duck circoviruses and development of molecular epidemiology.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Duck circovirus tandem repeated sequence and application thereof
ActiveCN110628765AStrong promotional valueStrong application valueMicrobiological testing/measurementDNA/RNA fragmentationPcr ctppVirus gene expression
The invention provides a duck circovirus tandem repeated sequence and application thereof and belongs to the technical fields of bioengineering and molecular biology. The invention firstly discovers aQTR sequence in which DuCV specifically exists, and the tandem repeated sequence does not appear in other circoviruses. QTR can serve as a gene I type and II type DuCV genetic typing new molecular marker; meanwhile, the invention proves the effect of regulating and controlling mRNA stability by the QTR serving as a DSE component by a dual-luciferase reporter system and a semi-anchored reverse mutation PCR technology, and defines the important effect of regulating and controlling virus gene expression by the QTR. The invention provides important technical and theoretical basis for researchingDuCV genotyping and virus replication regulation and control mechanism.
Owner:LINYI UNIVERSITY
Triple PCR detection kit for duck circovirus, duck adenovirus and duck tembusu virus
PendingCN114107556AQuick checkReduce workloadMicrobiological testing/measurementDNA/RNA fragmentationNucleotideTembusu virus
The invention relates to a triple PCR (Polymerase Chain Reaction) detection primer group for duck circovirus, duck adenovirus and duck tembusu virus. The triple PCR detection primer group comprises a first primer pair, a second primer pair and a third primer pair, wherein the first primer pair has a nucleotide sequence pair as shown in SEQ ID NO.1 and SEQ ID NO.2; the second primer pair has a nucleotide sequence pair as shown in SEQ ID NO.3 and SEQ ID NO.4; the third primer pair has a nucleotide sequence pair as shown in SEQ ID NO. 5 and SEQ ID NO. 6. The invention further provides a triple PCR detection kit for the duck circovirus, the duck adenovirus and the duck tembusu virus, the detection kit disclosed by the invention can be used for simultaneously detecting the mixed infection or single infection condition of the duck circovirus, the duck adenovirus and the duck tembusu virus, the workload is reduced, and the detection time is greatly shortened; and the purpose of rapidly detecting pathogens is achieved.
Owner:LIAOCHENG UNIV
Newcastle disease virus and duck circovirus fusion protein as well as coding gene and application thereof
ActiveCN103524624AGuaranteed physical propertiesImprove the level ofFungiBacteriaF proteinNewcastle disease virus NDV
The invention discloses newcastle disease virus (NDV) and duck circovirus (DuCV) fusion protein as well as a coding gene and application thereof. The fusion protein provided by the invention sequentially contains F protein of duck source newcastle disease virus and Cap protein of duck circovirus from an amino terminal to a carboxyl terminal; the amino acid sequence of the F protein of the duck source newcastle disease virus is the 1st to 553rd bits of a sequence 1 in a sequence table; the amino acid sequence of the Cap protein of the duck circovirus is the 574th to 829th bits of the sequence 1 in the sequence table. According to the invention, a Cap gene of the duck circovirus and an F gene of a duck source NDV isolate are used for constructing a fusion expression recombinant eukaryotic plasmid pcDNA3.1-F-Cap for the first time, the fusion expression recombinant eukaryotic plasmid pcDNA3.1-F-Cap is used as a DNA vaccine to immune SPF ducklings of 2 weeks old, and the result shows that the DNA vaccine can effectively promote the generation of an NDV antibody and a DuCV antibody by the SPF ducklings. The invention provides a theoretical and practical basis for researching a gene vaccine of DuCV and duck source NDV.
Owner:GUANGXI VETERINARY RES INST
A kind of cell-penetrating peptide derived from cap protein of duck circovirus and its design method and application
The present application discloses a cell-penetrating peptide derived from Cap protein of duck circovirus and its design method and application. The amino acid sequence of the cell-penetrating peptide is: LRRRRRRRRLRIARPRRRF; the cell-penetrating peptide of this application is derived from the Capsid structural protein of duck circovirus, which can significantly improve the transmembrane efficiency of transport targets such as nucleic acid immunopotentiators. It mediates Poly(I:C) and CpG ODN into macrophages to induce interferon IFN-β transcription levels to increase nearly 4 times that of TAT, and 8 times that of immunostimulators alone. The cell-penetrating peptide Du-Cap aa18-37 of the present application is safe and efficient, can significantly improve the transmembrane efficiency, further enrich the types of cell-penetrating peptides, and can be developed into a safe and efficient gene drug and nucleic acid immunity Ideal delivery vehicle for stimulants.
Owner:TAIZHOU UNIV
A rapid dual PCR method for genotype identification of duck circovirus
InactiveCN104059998BIncreased sensitivityAccurate identificationMicrobiological testing/measurementDNA/RNA fragmentationGenotypePcr method
The invention provides a duplex polymerase chain reaction (PCR) method for rapidly identifying duck circovirus genotype. The method comprises the following steps: comparing sequences DuCV-1 and DuCV-2 released on reference GenBank, selectively and respectively designing a pair of general specific primers SEQ1 / SEQ2 aiming at DuCV, a type 1 duck circovirus specific primer SEQ3 and a type 2 duck circovirus specific primer SEQ4 in a conserved region of the DuCV-1 and DuCV-2. According to the method, novel specific primers are designed and screened, various parameters in the reaction process are optimized, the dual PCR sequence-based typing method aiming at the duck circovirus established by utilizing the primers is high in specificity and high in sensitivity, and the sequences DuCV-1 and DuCV-2 can be rapidly and accurately identified.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Primer and probe combination for RF-RAA detection of duck circovirus and application thereof
PendingCN113846188AStrong specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationEpidemiologic surveyVirus detection
The invention relates to the technical field of virus detection, and particularly discloses a primer and probe combination for RF-RAA detection of duck circovirus and application thereof. In the primers and the probe for detecting the duck circovirus, the sequence of an upstream primer is shown as SEQ ID NO: 1, the sequence of a downstream primer is shown as SEQ ID NO: 2, and the sequence of the probe is shown as SEQ ID NO: 3. Specific primers and probes are designed according to a conserved motif of DuCV, an RF-RAA method applied to detection of DuCV is established, accumulation of amplification products is positively correlated with the intensity of fluorescence signals, an obvious result can be observed through an instrument after the reaction is performed for 15 min at 41 DEG C, and the method is high in specificity, high in sensitivity, high in accuracy, capable of achieving the lowest detection limit of 1 copy / microliter and capable of being applied to detection of DuCV. Reliable guarantee is provided for early clinical detection and epidemiological investigation of the duck circovirus, and positive significance is achieved on prevention and control of epidemic diseases.
Owner:HEBEI AGRICULTURAL UNIV.
A pcr-rflp method for distinguishing duck circovirus and goose circovirus
ActiveCN103602759BIdentification method is simpleImprove efficiencyMicrobiological testing/measurementMicroorganism based processesBiotechnologyDistinctin
The invention discloses a PCR-RFLP method for distinguishing duck circovirus and goose circovirus. The method utilizes the difference in the enzyme cutting site of the Rep protein gene sequence to distinguish, including DNA extraction and PCR amplification to obtain Rep The protein gene fragment was digested with XhoI and analyzed by RFLP. The identification method of the invention is simple, and the efficiency and accuracy are high.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Anti-duck circovirus single-chain antibody and preparation method and application thereof
ActiveCN111499734AImprove blockageGood virus recognition specificityBacteriaBiological material analysisPhage antibodiesSingle-Chain Antibodies
The invention discloses an anti-duck circovirus single-chain antibody and a preparation method and application thereof, and relates to the technical field of bioengineering. The amino acid sequence ofthe anti-duck circovirus single-chain antibody is Seq ID NO.1 or Seq ID NO.3. The invention provides a coding gene of the anti-duck circovirus single-chain antibody. The nucleotide sequence of the coding gene is Seq ID NO.2 or Seq ID NO.4. The anti-duck circovirus single-chain antibody ScFv-DuCV is screened by adopting a phage antibody library technology, antibody structure modification is further carried out on the anti-duck circovirus single-chain antibody ScFv-DuCV so that the anti-duck circovirus single-chain antibody ScFv-mDuCV is obtained, and the anti-duck circovirus single-chain antibody ScFv-DuCV and the anti-duck circovirus single-chain antibody ScFv-mDuCV can be used for duck circovirus detection and diagnosis. The anti-duck circovirus single-chain antibody ScFv-mDuCV and the duck circovirus have good virus recognition specificity, meanwhile, the anti-duck circovirus single-chain antibody ScFv-mDuCV has a good blocking effect on the duck circovirus, and a foundation is laidfor research of novel drugs for treating and inhibiting the duck circovirus.
Owner:青岛嘉智生物技术有限公司 +2
Inverse PCR amplification of duck circovirus gene
InactiveCN103642937AHigh purityEasy to operateMicrobiological testing/measurementDNA preparationRestriction enzyme digestionElectrophoresis
The invention discloses inverse PCR amplification of a duck circovirus gene. The inverse PCR amplification is characterized in that the inverse PCR amplification includes subjecting a PCR product to electrophoresis on agarose gel having a concentration of 2% and cutting a target fragment under a fluorescent lamp; recovering and purifying; dipping into an LB solution and performing blue / white selection to screen again; subjecting selected bacterial colonies to plasmid extraction, performing PCR again and performing restriction enzyme digestion identification; performing pre-degeneration in an environment having a temperature of 95-100 DEG C for 3-4 min; and then repeating the above step every 3 min for 25 times; agarose gel electrophoresis detection is performed again after the step is repeated for 25 times; and ice bath is performed for 3 min before electrophoresis detection. Beneficial effects of the inverse PCR amplification are that successful extraction of DNA in the duck circovirus genome, high purity, simple and convenient operation, low cost, strong repeatability and capability of meeting experiment standards are achieved.
Owner:李刚
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