SNP (Single Nucleotide Polymorphism) marker related to litter size of sow and application thereof

A technology of litter size and marking, applied in the field of animal genetics and breeding

Inactive Publication Date: 2011-06-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, pigs have not been GDF2 Genes as Molecular Markers for Reproductive Traits in Pigs

Method used

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  • SNP (Single Nucleotide Polymorphism) marker related to litter size of sow and application thereof
  • SNP (Single Nucleotide Polymorphism) marker related to litter size of sow and application thereof
  • SNP (Single Nucleotide Polymorphism) marker related to litter size of sow and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Collection of pig ear tissue samples and DNA extraction:

[0024] The samples were all from Large White sows from the Yaogu Pig Farm of Huanong Wenshi Animal Husbandry Co., Ltd.

[0025] Genomic DNA was extracted using the phenol-chloroform extraction method:

[0026] (1) Take about 50 mg of ear tissue, cut it into pieces, add 200 μL in cell lysate, mix with 100 μL 10×TE, add 2 μL of proteinase K, and place in a 55°C water bath overnight;

[0027] (2) After the digestion is complete (overnight), add 300 μL of chloroform to mix, shake up and down for 10-15 minutes (gently to ensure the integrity of the DNA);

[0028] (3) Centrifuge the mixture at 10,000 rpm for 2 min, then take about 200 μL of the supernatant and place it in a new 1.5 mL centrifuge tube

[0029] (4) Add 250 μL of precipitating solution to the centrifuge tube, mix well, leave at room temperature for 2 minutes, and then centrifuge at 10,000 rpm for 2 minutes;

[0030] (5) Pour off the supernatant, im...

Embodiment 2

[0042] to pig GDF2 Detection application of correlation analysis between different genotypes of gene G+701C locus and T+4578C locus and pig reproductive traits. pig GDF2 The analysis results of gene G+701C locus and T+4578C locus are shown in Table 1.

[0043] Table 1 Genotype frequency and allele frequency of SNP loci in Large White pig population

[0044]

[0045] It can be seen from Table 1 that at the G+701C locus, in the Large White pig population, the GG homozygous type is much higher than the CC type and GC type, and the G allele is the dominant gene. T+4578C locus, CC>TC>TT in Large White pigs, the distribution frequency of C allele is also far greater than that of T allele.

[0046] Table 2 Pig GDF2 Association Analysis of Gene G+701C Locus and T+4578C Locus and Pig Reproductive Traits

[0047]

[0048] Remarks: 1: Least square mean ± standard error; the same letter in the table indicates no significant difference, different lowercase letters indicate signi...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to the litter size of a sow and application thereof. The SNP molecular marker provided by the invention is positioned on G+701C and T+4578C sites of a GDF2 (Growth Differentiation Factor 2). By designing the primers, the two obtained sequences are respectively positioned on Exons 1 and 2 of the gene GDF2 of the sow, the 701bp of the gene GDF2 (ENSSSCG00000010375) sequence recorded in the Ensemble database has one G701-C701 base mutation and results in the change (Ala-114-Pro) of the amino acid, and the 4578bp has one T4578-4578C base mutation which is a synonymous mutation. An RFLP-PCR (Restriction Fragment Length Polymorphism-Polymerase Chain Reaction) method is used for researching the polymorphism of the site. The invention provides a new marker for molecular marker-assisted selection of sows.

Description

technical field [0001] This law relates to the field of animal genetics and breeding, specifically SNP markers related to sow litter size and their applications. Background technique [0002] my country is the world's largest pig raising country and a big pork consumer. The pig industry occupies a pivotal position in my country's animal husbandry industry. The pig industry accounts for about 35% of the value of the animal husbandry industry and is the main source of income for the animal husbandry industry. [0003] As an important economic trait, reproductive traits directly affect the economic benefits of pig farming. Traditional quantitative genetics has played an important role in guiding livestock breeding, but reproductive traits have low heritability, late performance of traits, and limited inheritance, so it is difficult to make progress through conventional selection. To further improve the reproductive performance of pigs, a better understanding of its genetic me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 周晓宁聂庆华张细权贾新正
Owner SOUTH CHINA AGRI UNIV
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