7 results about "Restriction enzyme digestion" patented technology

1 µL each restriction enzyme. 15 µL sterile water. Incubate the reaction at digestion temperature (usually 37°C) for 1 hour. Stop the digestion by heat inactivation (65°C for 15 minutes) or addition of 10mM final concentration EDTA. The digested DNA is ready for use in research applications.