205results about How to "Resolve source issues" patented technology

The invention discloses a biological production method of tanshinol, which comprises the following steps: synthesizing p-hydroxyphenylpyruvic acid into 3,4-dihydroxyphenylpyruvic acid under the catalytic action of a p-hydroxyphenylacetic acid meta-position hydroxylation enzyme, and then, synthesizing to obtain the tanshinol under the catalytic action of a D-lactate dehydrogenase; or synthesizing p-hydroxyphenylpyruvic acid into p-hydroxyphenyllactic acid under the catalytic action of a D-lactate dehydrogenase, and then, synthesizing to obtain the tanshinol under the catalytic action of a p-hydroxyphenylacetic acid meta-position hydroxylation enzyme. According to the invention, gene engineering glutamic acid corynebacteria and Escherichia coli are used to produce the tanshinol through fermentation, and the tanshinol can be synthesized without adding a substrate, thereby realizing de novo synthesis of the tanshinol and solving the problem on the source of the tanshinol; and meanwhile, the production cost is lowered to the greatest extent. Thus, the biological production method is beneficial to industrial production.

The invention relates to a comprehensive utilization method of straw raw materials. The method is characterized by comprising the following steps: a, carrying out solid-liquid separation on steam-exploded straws, using an alkaline cooking method to process solid bodies of the steam-exploded straws, and extracting good xylogens from an alkaline extract liquor by an acid-out method; b, carrying out enzymolysis on the extracted residues in the step a to obtain zymolytic residues and a zymolytic liquid, wherein the zymolytic residues are used for preparing pulps; and c, after the steam-exploded straws in the step a are detoxificated, mixing a zymolytic liquid with the zymolytic liquid in the step b and adding nitrogen sources to butylicum fermentation. The method has the advantages that sources of raw materials are wide; the separation of celluloses (continuous fibers and staple fibers), hemicelluloses, xylogens and the like in the straws is realized, thus sufficiently realizing the value of each component; and the celluloses are divided into the continuous fibers which are difficult to realize enzymolysis and the staple fibers which are easy to realize enzymolysis, thus reducing the usage amount and cost of the cellulase, improving the comprehensive utilization value of the straws and solving the source problem of raw materials in industrialization production of the butylicum fermentation.

The invention provides a method for culturing mushroom using pig nightsoil comprising culturing the mushroom using pig nightsoil instead of the cow dung in the conventional mushroom culture medium. The cultivation steps are as follows: culture medium preparation- stacking prewet- primary fermentation- post fermentation- cooling- sowing- fungi growth and soil cover- management of producing mushroom- mushroom picking- mushroom product. By using the pig nightsoil instead of cow dung to culture mushroom, the method of the invention solves the raw material source problem of the mushroom scale production, reduces the production cost of the mushroom, is useful for the sustainable development of the edible fungi industry, improves the production efficiency of the edible fungi, further more achieves the resource utilization of the pig nightsoil, improves the utilization value of the pig nightsoil, reduces the pollution discharge in the pig industry and is useful for quantitative reduction and resource reutilization in the pig industry.

The invention belongs to the technical field of recycling of resources, and in particular relates to a method for biologically purifying marsh gas and recycling waste of the marsh gas. The method comprises the following steps of: purifying carbon dioxide in the marsh gas by using a photo-bioreactor constructed by algae to obtain high-quality biomass energy methane; simultaneously, combining fermented liquid and residues which are residual after fermentation, and comprehensively recycling the algae proliferated in the photo-bioreactor to fulfill the aim of zero emission of the waste. By the method, the marsh gas has an obvious purification effect, the concentration of the methane in the marsh gas can be improved from the range of between 30 and 40 percent to 90 percent, the energy flow of solar energy from the algae to high-quality marsh gas and the matter cycle of nutrient elements such as C, N, P and the like are realized, and economic and social benefits are obvious.

The present invention relates to a group of glycosyl transferase and applications thereof, and specifically provides applications of the glycosyl transferase gGT29-7 and a derived polypeptide thereof in terpene compound glycosylation catalysis and new saponin synthesis, wherein the glycosyl transferase can specifically and efficiently transfer the glycosyl from a glycosyl donor to the first glycosyl on C-3 site and/or C-6 site of a tetracyclic triterpene compound so as to extend the sugar chain. The glycosyl transferase of the present invention can further be used for constructing artificial ly-synthesized rare ginsenosides and a variety of new ginsenosides and derivatives thereof.

The invention relates to a group of glycosyl transferases and applications of the glycosyl transferases. Particularly, the invention provides applications of glycosyl transferases gGT25, gGT13, gGT30, gGT25-1, gGT25-3, gGT25-5, gGT29, gGT29-3, gGT29-4, gGT29-5, gGT29-6, gGT29-7, 3GT1, 3GT2, 3GT3 and 3GT4, and derived peptides of the glycosyl transferases in glycosylation catalysis and new saponin synthesis of terpenoids. The glycosyl transferases can specifically and efficiently catalyze hydroxyl glycosylation of C-20 bit and/or C-6 bit and/or C-3 of a tetracyclic triterpenoid compound substrate, and/or transfers the glycosyl groups from glycosyl donors to the first glycosyl groups of the C-3 bit and C-6 bit of the tetracyclic triterpenoid compound to extend a carbohydrate chain. The glycosyl transferases disclosed by the invention can also be applied to building synthetic rare ginsenosides and a plurality of novel ginsenosides and derivatives of the ginsenosides.

The invention relates to a method for cultivating the Krause's crayfish, comprising that cleaning the field, cultivating the parental crayfish, cultivating the seed and taking off, cultivating the rice. The invention is characterized in that the cultivation of parental crayfish is processed in the surrounded groove, which has an anti-jump device and near the ground arranges seed holes at 0.8-1.2cm diameter; the crayfish seed can pass through the holes into the field. The invention can pre-cultivate the parental crayfish to avoid damaging the rice. And the invention can obtain many benthic fauna as the bait of parental crayfish, seed, or the like.

The invention discloses a biological method for producing resveratrol, which comprises the following steps: ligating target genes obtained by restriction enzyme digestion to an expression vector, wherein the target genes include the sequences of phenylalanine hydroxylase (PAH), tyrosine ammonia lyase (TAL) 4-coumarate:coenzyme A ligase (4CL) and resveratrol synthase (RS); transforming the constructed expression vector into a strain to obtain a recombinant engineered strain; and fermenting the recombined engineered strain. Compared with the prior art, the technical scheme provided by the invention uses the genetically engineered strain for fermentation to produce resveratrol, so as to realize denovo synthesis of resveratrol with no need of adding substrates. The method disclosed by the invention solves the source problem of resveratrol, and at the same time, reduces the production cost in a maximum extent, and is beneficial for industrial production.

The invention discloses an ice preventing and removing system for an aircraft wing. The system comprises a waste gas treatment device, an air pump, a shell, an evaporator, a condenser, an air line and a liquid line, wherein engine waste gas treated by the waste gas treatment device is introduced into the shell to heat the evaporator, working media are vaporized after being heated in the evaporator and then led out through the air line, and then the working media are cooled into liquid in the condenser located on the skin and give out heat, and finally flow back to the evaporator through the liquid line to finish the whole circulation. According to the system, the vapor-liquid two-phase flow phase change heat transfer design concept is adopted, vapor-liquid phase conversion of the working media is utilized to transmit heat, and then the purpose of preventing and removing ice on the aircraft wing is achieved; engine waste gas of an airplane is utilized as the heat source to increase the comprehensive utilization rate of airplane energy.

The invention relates to CO2 emission reduction and recycling technologies, and discloses a method for adopting microalgae culture immobilized microbe fermentation tail gas CO2 as a supplementation fermentation raw material. According to the invention, tail gas in a microbe fermentation tank is directly introduced into a microalgae culture illumination bioreactor; conditions such as illumination, temperature, and ventilation are controlled; proper inorganic nutrient salts are added; CO2 in microbe fermentation tail gas is adopted as a carbon source for culturing microalgae; biomasses such as carbohydrates and proteins are accumulated; the biomasses are converted into raw materials such as carbon and nitrogen sources needed by fermentation through pretreatment; and the raw materials are delivered back to the microbe fermentation system to be utilized as a fermentation supplementation raw material. With the method provided by the invention, emission reduction and recycling of the microbe fermentation tail gas CO2 are realized; grain raw material consumption of microbe fermentation is reduced; and good environmental and social benefits are provided.

The invention discloses an artificial conglomerate core which is used an indoor physical simulation experiment, and belongs to the technical field of oil-gas field development physical simulation. The artificial conglomerate core is mainly prepared from the following raw materials in percentage by weight: 2-15% of clay, 5-25% of cementing matter, 20-50% of river sand and 30-60% of quartz sand. The artificial conglomerate core has the advantages that the source problem of the conglomerate core in physical simulation is solved, the obtained artificial conglomerate core is stable in structure, complicated in pore throat structure and diverse in core components, has the characteristics of simple structure and stable property and can replace natural core to be used in physical simulation, and the shortcomings that the pore throat disposition, wetting property and the like of the synthetic core have great difference from those of the natural core are effectively overcome.

The invention relates to a method for preparing an environment-friendly thermal-insulation building block from construction waste, which comprises the following steps: uniformly mixing cement, active mineral admixture, haydite and construction waste in a weight ratio of (8-25):(20-60):(20-45):(8-15), adding water, pulping and stirring for 5-10 minutes, adding a special additive which accounts for 0.8-1.5 wt% of the dry powder, and stirring for 0.5-1 minute; and preparing a slurry, injecting into a specimen mold, standing in a 55 DEG C constant-temperature steam curing chamber, curing for 4 hours, demolding, cutting with a steel wire, sending into an autoclave, heating and pressurizing for 8 hours, and discharging to obtain the thermal-insulation building block. The density of the thermal-insulation building block is 500-700 kg/m<3>, the heat conductivity coefficient is less than or equal to 0.16 W/m.K, and the compression strength is greater than or equal to 5 MPa. By using the construction waste instead of part of the active mineral admixture and the haydite as the component, the method achieves the effects of protecting the environment, enhancing the cyclic utilization efficiency of resources and lowering the cost.

The invention relates to a Pleurotus eryngii culture medium which is prepared from the following raw materials in parts by mass: 40-60 parts of bacterial dreg, 30-40 parts of corn cob, 10-15 parts of bran and 0.9-1.1 parts of lime. By using the bacterial dreg instead of wood dust and cottonseed hull, the bacterial dreg is recycled, thereby enhancing the bioavailability of the effective constituents in the bacterial dreg; and thus, the invention solves the problem of raw material resources, effectively utilizes the agricultural leftovers, changes wastes into valuable substances and lowers the raw material cost and transportation expenses, thereby lowering the production cost of the Pleurotus eryngii and having favorable economical and ecological effects.

The invention provides the gene of an antibacterial peptide. The gene is used for coding the antibacterial peptide with an amino acid sequence represented by SEQ ID No.1, and possesses one of the nucleotide sequences represented by SEQ ID No.2-4. The invention also provides a preparation method of the antibacterial peptide. The preparation method comprises following steps: (1) an expression system containing one of the nucleotide sequences represented by SEQ ID No.2-4 is constructed, wherein expression carriers are constructed, and cell transformation of host cells is realized by the expression carriers so as to obtain recombinant cells capable of realizing expression of the antibacterial peptide; (2) the recombinant cells are cultured so as to realize expression of the antibacterial peptide; and (3) the expression products are separated and purified so as to obtain the antibacterial peptide with the amino acid sequence represented by SEQ ID No.1. The antibacterial peptide can be used as an antibacterial agent in the fields of agriculture, food, hygienic products, medicine, cosmetic, biopesticide, biological feed additive, natural food antiseptics, and zoological and botanical resistance gene engineering.

The invention discloses a tailings disposing and filling method and belongs to the field of tailings utilization and disposal. The method includes: grading tailing pulp to obtain fine-particle tailings pulp and coarse-particle tailings; dewatering the fine-particle tailings pulp and the coarse-particle tailings pulp respectively according to application to obtain fine-particle tailings and fine aggregate and coarse-particle tailings for granulation, mortar and dry-mixed mortar; sintering the fine-particle tailings at high temperature, and adding a binding material into the coarse-particle tailings for granulation, mortar and dry-mixed mortar to obtain coarse aggregate, a mortar material and a dry-mixed mortar material; mixing the coarse aggregate and the fine aggregate, conveying the samedown into a well through a filling well, conveying the mortar material down into the well through a filling pipe, mixing, and then filling; conveying the dry-mixed mortar material down into the well to be mixed with pipe flushing water to prepare a dry-mixed mortar material mixture, mixing the mixture with the aggregate, and filling. By the method, treating and hardening time of the filling material can be shortened, strength of a filled body can be enhanced, downhole operation conditions can be improved, and pollution of underground environment can be reduced.

The invention provides a method for preparing 4-tert-octylphenol. The C4 fraction addition mixture with 80 percent of isobutene dimer remained by esterification-degasification of sulfuric acid is taken as the raw material ; after the raw material is braized and cut under the temperature between 75 DEG C and 105 DEG C, the isobutene dimmer that the content of the target distillate is more than 98 percent is obtained to be taken as the alkylating agent; Na-type sulfonated styrol-butadiene polymer strong base anion-exchange resin with 50 percent of water is acidified to a H-type sulfonated styrol-butadiene polymer strong acid cation-exchange resin as the alkylation catalyst; the target distillate isobutene dimmer and the phenol are made a alkylation reaction under the temperature between 90 DEG C and 100 DEG C with the H-type sulfonated styrol-butadiene polymer strong acid cation-exchange resin as the catalyst to produce the 4-tert-octylphenol alkylation mixing liquid; the alkylation mixing liquid is braized and cut under the temperature between 220 DEG C and 270 DEG C to produce the 4-tert-octylphenol.

The invention discloses a method for performing coal mining and gangue cementation stripe filling simultaneously. When a working surface advances to a certain distance, an excavator and filling equipment are delivered under a filling support for assembly and adjustment; a bottom excavator excavates a bottom while coal mining machine mines coal, underlying strata excavated by the excavator are delivered to a filling bag through a filling system; the underlying strata form a first filling strip of a support goaf top plate after solidifying and sizing; and after filling of the first filling strip, the filling operation of a second filling strip is performed; and the steps are repeated accordingly, and coal mining and filling are performed simultaneously until a coal face is mined. According to the method, the filling mechanical equipment is transferred under a well, so that the ground site arrangement is reduced, gangue of down hole bottom excavating board is fully used, the problem of the filling material source is solved, transport links of filling materials are reduced, the filling system moving with the working surface simultaneously is formed, down hole filling is rapid and effective, and simultaneously, an efficient work flow of coal mining and filling simultaneously can be achieved.

The invention relates to a CAR-NK cell as well as a preparation method and an application thereof. The method comprises the following steps: S1, linking a specific antibody coding gene into a cloning site region of a modified CAR skeleton so as to obtain a CAR gene, wherein the modified CAR skeleton comprises a CD244 episporium signal region, the cloning site region, a CD244 extracellular hinge region, a CD244 transmembrane part region and a CD244 intracellular part region which are sequentially connected; and S2, infecting the CAR gene with NK cell through lentivirus, so that the NK cell expressing the CAR protein is obtained, namely the CAR-NK cell. The prepared tumor immnuotherapy cell, namely the CAR-NK cell, of the invention, besides a patient with cancer, is also applicable to allosome infusion; therefore, the prepared tumor immnuotherapy cell, in comparison with existing CAR-T, is wider in application scope and is lower in preparation cost.

This invention blow-stop ventilation device of a bottom blow gas supply element of a multi-blow converter is composed of an air dehydrator, a pneumatic fast cut valve, a pneumatic regulation valve, a gas flowmeter, a pressure tester, a computer control system, a blow-step gas source and gas transportation pipe lines interconnected by the gas transportation pipe lines. Said method takes air as the gas supply element and utilizes 20% oxygen in the air to oxidize the blockage-slag-metal mushroom head continuously to keep it at reasonable state to ensure the life time of the supply element the same with the converter lining.

The invention discloses a silk polished lacquer painting and a preparation method thereof. The silk polished lacquer painting is prepared by the following steps: (1) firstly dyeing, drawing, inlaying, embedding, pasting and etching on a silk surface with polished lacquer painting materials or carrying out digital jet-printing on the silk surface to form an initial product; (2) fixing and drying the initial product on the silk surface and then adjusting a picture; and (3) carrying out lacquering, polishing and refining on the dried initial product to obtain the required silk polished lacquer painting finished product. By preparing the silk polished lacquer painting in the invention, the range of used materials of the polished lacquer painting is expanded, the manufacturing period of the product can be reduced, and the silk polished lacquer preparation and industrialization production in inlaying, embedding, pasting and carving materials are realized.

The invention relates to a method for extracting chitosan using fly, characterized in that the fly puparium is treated by acid alkaline process to obtain raw material. The raw material is subjected to decalcifying, deproteinizing, deacetylating, and then filtering, cleaning to be neutral to obtain the chitosan end product. The method can produce chitosan in enormous quantities using the high breeding property of fly, therefore the production rate of chitosan is increased. A product center combining raw material production and chitosan production together can avoid the inconvenience of raw material collection, therefore the production scale is greatly increased and the cost is reduced. During feeding the fly, the fly can be used for extract of protein feedstuff and antibiotic fly protein.

The invention provides mytilus edulis polysaccharide extracted from mytilus sp., an industrial extraction method of the mytilus edulis polysaccharide, and an application of the mytilus edulis polysaccharide in preparation of antilipemic and antiatherosclerotic drugs and health food. The mytilus edulis polysaccharide takes (1-4)-alpha-D-Glc as main chains, contains (1-2)-alpha-D-Glc branches, and has a configuration of alpha-pyranoid glucan; every 12 glucose main chains averagely contain one (1-2) glucose branch; and the relative molecular weight is 800-8000KDa. The mytilus edulis polysaccharide is extracted from boiled and dried mytilus sp. by coarse extraction, enzymolysis, purification, drying and the like, and is mild in extraction condition and high in purity. The mytilus edulis polysaccharide can significantly reduce triglyceride, total cholesterol and low-density lipoprotein cholesterol, has an effect of reducing lipid, and is widely applied to preparation of drugs for treating or preventing hyperlipoidemia and atherosclerosis and preparation of antilipemic health products or special medical formula food.

The invention provides an electronic lock system with an electronic lock (2) being supplied electric energy and key information by a mobile (1). A battery (2) which is arranged in the mobile phone (1) is utilized to supply the electric energy to a wired or wireless electronic lock (2). A keyboard of the mobile phone (1), a touch screen of the mobile phone (1) or equipment which stores the key information of the mobile phone (1) are utilized to supply the key information to the electronic lock (2). The equipment of the mobile phone (1) generates or stores the key information and transmits the key information to the electronic lock (2) through a mode of wired contact or wireless transmission. Therefore, the same mobile phone (1) not only can solve the problem of electric energy supply to the electronic lock (2), but also supply the key information of unlocking to the electronic lock (2), roles of the mobile phone are further played, and the electronic lock is enabled to be further utilized conveniently. The electronic lock system with the electronic lock being supplied the electric energy and the key information by the mobile is beneficial to popularization of the electronic lock, and provides a great convenience for life of people.

The invention relates to a system and method of coproduction of propylene oxide and arene. A solvent namely methanol in the production course of the propylene oxide is used for the course of preparinghydrocarbon with the methanol, and besides, byproducts of hydrogen gas and propylene in the course of preparing the hydrocarbon with the methanol are used for preparing a hydrogen peroxide solution,so that the source problem of the hydrogen peroxide solution is solved; and a product byproduct namely the propylene is used as the raw material of the propylene oxide, so that the production technology and the energy consumption of the propylene oxide are reduced. Through the adoption of the system and method disclosed by the invention, steam consumption of production of the propylene oxide is substantially reduced, and the reduction of the steam consumption quantity reaches 50% or above.

A process for preparing potassium sulfate from seawater includes preparing the K-enriched liquid, in which the ammonium sulfate solution is used to elute out the K ions adsorbed by the clinoptilolite in ion exchange column, and preparing potassium sulfate, in which the ammonia is filled in said K-enriched liquid for educing out potassium sulfate. It features no pollution to environment.