Mytilus edulis polysaccharide, and preparation method and application of mytilus edulis polysaccharide

A mussel polysaccharide and mussel technology, applied in the field of mussel polysaccharide and its preparation, can solve problems such as unclear chemical structure, lipid-lowering efficacy and toxicity evaluation, and achieve high purity, high safety, and prevention of hyperlipidemia Effect

Active Publication Date: 2017-08-18
SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the chemical structure of mussel polysaccharide is not yet clear, and its lipid-lowering efficacy and toxicity evaluation also affect its further application in medicine and health food

Method used

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  • Mytilus edulis polysaccharide, and preparation method and application of mytilus edulis polysaccharide
  • Mytilus edulis polysaccharide, and preparation method and application of mytilus edulis polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Extraction and preparation of mussel polysaccharide.

[0045] (1) Raw material processing: heat thick-shelled mussels produced in Shengsi County, Zhejiang Province, remove the shells and leave the meat to dry, pulverize them with a pulverizer, and pass through a 20-mesh sieve to obtain dry mussel powder;

[0046] (2) Polysaccharide extraction: add 6 times the volume of water to the dried mussel powder prepared in step (1), and extract at 100°C for 2 h;

[0047] (3) Slag removal: use a 50-mesh screen to separate mussel meat residue and polysaccharide extract;

[0048] (4) Enzymolysis: Adjust the pH of the polysaccharide extract prepared in step (3) to 8.0 with NaOH, add alkaline protease with a mass ratio of 0.2%, and enzymolyze at 50 °C for 2 h, and then heat it at 80 °C after the enzymolysis is completed. Inactivation at high temperature for 20 min to obtain the enzymatic hydrolyzate;

[0049] (5) Filtration: use diatomaceous earth as a filter aid, and filt...

Embodiment 2

[0058] Example 2 Structural identification of mussel polysaccharides.

[0059] 2.1 Chemical analysis

[0060] The total sugar content was determined by the sulfuric acid / phenol method: draw 1 mL of mussel polysaccharide solution with a concentration of 100 μg / mL, add 1 mL of 6 % phenol solution, quickly add 5 mL of concentrated sulfuric acid, oscillate to mix well, and soak in boiling water for 20 min. , Cool to room temperature and measure its absorbance at a wavelength of 490nm. Using glucose concentration-absorbance as a standard curve, the linear regression equation is: Y=3.8108X+0.0415 (R 2 =0.9996), to calculate the total sugar content of the sample.

[0061] The content of uronic acid was determined by sulfuric acid / carbazole method: draw 0.5 mL of mussel polysaccharide solution with a concentration of 50 μg / mL and blank reagent. Add 3 mL of sodium tetraborate-sulfuric acid solution in an ice-water bath, boil in water for 15 min, take it out and immediately cool to r...

Embodiment 3

[0104] Example 3 Study on the hypolipidemic efficacy of mussel polysaccharides.

[0105] 3.1 Test animals

[0106] Male SD rats were selected from Jinan Pengyue Experimental Animal Breeding Co., Ltd., animal production license number: SCXK (Lu) 20140007. Animal certificate number: No.37009200002065, body weight 170±10 g.

[0107] 3.2 Test method

[0108] They were randomly divided into 6 groups, namely blank group, model group, L1 group (100 mg / kg), L2 group (200 mg / kg), L3 group (400 mg / kg), L4 group (800 mg / kg). All rats were fed with basic feed for observation for 1 week, weighed after fasting for 12 hours, blood was collected from the inner canthus of the eyes, left at room temperature 25 ℃ for 1 hour, centrifuged at 3000 rpm for 10 minutes, serum was taken, and TC and TG were measured with an automatic biochemical analyzer , HDL-C and LDL-C levels. The blank group was fed with basic feed all the time, and the rats in other groups were fed with high-fat feed (the formu...

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Abstract

The invention provides mytilus edulis polysaccharide extracted from mytilus sp., an industrial extraction method of the mytilus edulis polysaccharide, and an application of the mytilus edulis polysaccharide in preparation of antilipemic and antiatherosclerotic drugs and health food. The mytilus edulis polysaccharide takes (1-4)-alpha-D-Glc as main chains, contains (1-2)-alpha-D-Glc branches, and has a configuration of alpha-pyranoid glucan; every 12 glucose main chains averagely contain one (1-2) glucose branch; and the relative molecular weight is 800-8000KDa. The mytilus edulis polysaccharide is extracted from boiled and dried mytilus sp. by coarse extraction, enzymolysis, purification, drying and the like, and is mild in extraction condition and high in purity. The mytilus edulis polysaccharide can significantly reduce triglyceride, total cholesterol and low-density lipoprotein cholesterol, has an effect of reducing lipid, and is widely applied to preparation of drugs for treating or preventing hyperlipoidemia and atherosclerosis and preparation of antilipemic health products or special medical formula food.

Description

technical field [0001] The invention belongs to the field of natural product extraction and application, and relates to a mussel polysaccharide and its preparation method and application. Background technique [0002] Hyperlipidemia caused by lipid metabolism disorders is closely related to the occurrence of cardiovascular and cerebrovascular diseases, the number one killer of human health, and is one of the most important pathogenic factors that threaten human life and health. Among the popular blood lipid-lowering drugs currently on the market, statins are the most widely used and have the best curative effect, accounting for more than 80% of the total sales of blood lipid-lowering drugs. However, statins have adverse reactions related to liver, kidney, and metabolic disorders in the body. In addition, gastrointestinal reactions, rhinitis, sinusitis, headache, sore throat, influenza syndrome, arthritis, chest pain, insomnia, and muscle toxicity are common. and other toxic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61P9/10A61P3/06A23L33/125
CPCA23V2002/00C08B37/0003C08B37/0009A23V2200/3262A23V2250/51
Inventor 刘飞张金华张天娇侯重文程艳玲邵华荣朱希强凌沛学解荣利张辉
Owner SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES
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