The invention discloses high-temperature self-gelation and baking-resistant cake stuffing pre-mix powder and preparation and application thereof. The cake stuffing pre-mix powder comprises the following components: 40-50 percent pre-gelatinized starch, 12-15 percent methyl cellulose, 1-12 percent xanthan gum, 20-25 percent sorbitol powder, 8-15 percent powdered sugar and 3-5 percent corn starch. The cake stuffing is in a half-solidification state and has a certain viscosity when being stirred with the pre-mix powder; the situation that a feeding head leaks due to the good liquidity of paste is avoided when the paste is filled in a paste filling machine; meanwhile, the viscosity degree can meet the requirements of normal extrusion of the paste filling machine, and the normal production efficiency is not affected; during the baking, the half-solidification stuffing paste can be automatically solidified into a solid state, the overflowing boiling phenomena of the stuffing can be reduced,a cavity can not be formed in the cake, and the organization and appearance of the cake can not be affected; and the water in the baked stuffing can not transfer to the cake embryo, so that the watercontent in the cake embryo can not increase to affect the guarantee period of the cake. According to the invention, the cake paste is first filled in the cake stuffing, and then the high-temperature baking step is carried out, so that the guarantee period risks of the cake are greatly reduced.