The application discloses a digital
microfluidic chip integrating
myocardial tissue culture and multimode detection, and belongs to the
microfluidic chip field.The digital
microfluidic chip comprises a lower substrate, an upper substrate and a sealing
gasket; the lower substrate and the upper substrate are connected through the sealing
gasket around the lower substrate and the upper substrate, and a space for
liquid drop flow is formed in the middle; the lower substrate is provided with a functionalized partition
electrode array, which comprises a
liquid storage area, an electrical physiological acquisition area and a
biochemical detection area; the electrical physiological acquisition area adopts an MEA electrical physiological acquisition array and is used for acquiring field potential signals of
myocardial tissue in situ; a recess for accommodating a
solid-phase carrier is formed in an
electrode of the
biochemical detection area, and a myocardial injury marker is captured through the
solid-phase carrier; the upper substrate is made of a transparent material and is provided with a channel communicating with the
liquid storage area and the electrical physiological acquisition area; and the digital microfluidic
chip drives liquid drops through a
dielectric wetting effect, integrates
myocardial tissue culture, electrical physiological
signal monitoring and biochemical marker detection on the same
chip, and realizes in-situ
synchronous detection of electrical
physiology and
biochemistry in a dual-mode manner.