11 results about "Acidithiobacillus" patented technology

The invention discloses a method for treating red mud by using waste sulfuric acid, which comprises the following steps: mixing waste sulfuric acid and red mud, placing the mixture in a double-chamber electrochemical reaction system, breaking the silicate structure of the red mud in an anode chamber through 0.8-1.0 V voltage, and introducing acidithiobacillus acidophilus for metabolism to generate oxalic acid and citric acid to dissolve iron and aluminum oxides; the extracellular polymer fixes heavy metal ions to reduce the leaching toxicity by 90%, a cathode chamber is blocked by an ion exchange membrane to recycle more than 85% of waste acid, step-by-step precipitation of Fe < 3 + >, Al < 3 + > and Ca < 2 + > is realized by regulating and controlling the pH gradient in stages, goethite with the magnetic separation purity being greater than or equal to 95% and aluminum gel are used as functional materials, and 99% high-purity gypsum is generated through induction of nanocrystalline seeds. According to the method, 95% of iron recovery rate and heavy metal stabilization in the red mud are achieved, the waste acid recycling rate reaches 85%, the method has the advantages of low energy consumption and recycling, the treatment cost is reduced by 50% or above compared with a traditional method, and an efficient solution is provided for cooperative treatment of the red mud and the waste acid.

According to the efficient composite ore leaching flora and the application thereof, by optimizing the specific proportion of thiobacillus ferrooxidans, thiobacillus acidophilus, thiobacillus thiooxidans and high-temperature-resistant leptospirillum and combining a directional domestication technology, the tolerance of the flora to ores with high toxicity and complex components is remarkably improved. Extracellular polymeric substances secreted by the flora strengthen mineral surface reaction through chelation, and the copper leaching rate is increased by 40% or above compared with that of a single strain in cooperation with a segmented temperature control process. The flora provided by the invention can realize efficient metal recovery under the condition of low energy consumption, is suitable for large-scale dump leaching and reactor leaching, and has dual advantages of environmental protection and economy.

The invention discloses application of a microbial composition in industrial hazardous waste treatment, and belongs to the technical field of industrial hazardous waste treatment.The application comprises the following steps that S1, industrial hazardous waste is collected, the industrial hazardous waste is pretreated to obtain a hazardous waste material, and a fermentation tank is filled with the hazardous waste material; s2, adding a growth culture medium into a bioreactor, inoculating the domesticated bacterial liquid into the bioreactor, and carrying out enlarged culture to obtain a fermentation material of the composite acidophilic thiobacillus; s3, adding a biosurfactant into the fermentation material of the composite acidophilic thiobacillus, and uniformly stirring to obtain the composite acidophilic thiobacillus with hydrophobically modified cell membrane surface; and S4, adding an acidic pH buffer solution to obtain a to-be-treated material. According to the method, in the industrial hazardous waste treatment process, the stability of the pH required by the acidithiobacillus acidophilus can be maintained, and the cell membrane surface hydrophobicity of the composite acidithiobacillus acidophilus is improved.

The invention discloses a microbial flora for treating sulfate wastewater and a preparation method of the microbial flora. Comprising the following microbial treatment flora components: 10-35% of biphasic photosynthetic bacteria, 5-10% of sulfate reducing bacteria, 5-30% of bifidobacterium, 3-8% of desulfurization vibrio, 5-25% of mesophilic actinomycetes, 2-5% of methanogens, 10-30% of sub-digestion bacteria, 2-6% of acidithiobacillus acidophilus, 12-30% of bacillus megatherium, 1-4% of iron-oxidizing bacteria, 1-5% of denitrifying bacteria and 1-3% of phosphorus-accumulating bacteria. According to the method, sulfate is efficiently removed, the removal rate of SO4 < 2-> is larger than or equal to 85%, the H2S output is reduced by 70% or above, multi-pollutant cooperative treatment of odor pollution is avoided, the removal rate of COD is larger than or equal to 60%, the removal rates of total nitrogen and total phosphorus are larger than or equal to 50% and 40% respectively, the comprehensive discharge standard is met, the environmental adaptability is high, the salt tolerance NaCl is smaller than or equal to 5%, the temperature fluctuation resistance is 20-40 DEG C, and the method is suitable for the complex environment of industrial wastewater, low in cost and suitable for industrial production. Extra chemical agents are not needed, strains can be bred autonomously, and the operation cost is reduced by 40% or above compared with a traditional method.

The present invention discloses a method for improving the acid tolerance of E. coli by integrating the molecular chaperone CbpA of extreme acidophilic bacteria, belonging to the fields of biochemistry and molecular biology. The present invention provides a molecular chaperone protein CbpA from Acidithiobacillus thermophilum acidophilus and its encoding gene; the molecular chaperone protein CbpA can improve the acid stress resistance of Escherichia coli, and through gene editing technology, the genome of E. coli BL21 is modified to obtain recombinant Escherichia coli. By detecting the growth status and organic acid tolerance of wild-type BL21, defective BL21-ΔcbpA, and recombinant strain BL21-ΔcbpA / AccbpA strains under acidic conditions, its acid tolerance was confirmed. The present invention lays a foundation for later improving the acid tolerance performance of engineering strains through macromolecular repair means such as molecular chaperone proteins, so as to improve the robustness of industrial microorganisms.

The invention discloses a method for bioleaching a retired ternary lithium battery positive electrode material under combined catalysis of an extracellular polymer and Cu < 2 + >, which comprises the following steps: (1) discharging, roasting, disassembling and grinding a retired ternary lithium battery to obtain ternary lithium battery positive electrode material powder; (2) carrying out mixed culture on the Acidithiobacillus ferrooxidans and the Acidithiobacillus thiooxidans, so as to obtain a mixed bacterial solution; (3) extracting an extracellular polymeric substance from the mixed bacterial liquid obtained in the step (2), and adding the extracellular polymeric substance and Cu < 2 + > into the mixed bacterial liquid obtained in the step (2) to obtain a biological leaching agent; and (4) the waste ternary lithium battery powder obtained in the step (1) is added into the biological leaching agent prepared in the step (3) for a biological leaching reaction for 3-7 days, and a biological leaching solution is obtained. According to the method, the bioleaching rate of metal is increased through the extracellular polymeric substance and Cu < 2 + >, operation is easy and convenient to implement, and the catalyst is simple and easy to obtain.

The present invention relates to bioleaching of tailings and concentrates at temperatures above 30°C to obtain cobalt and other metals, using a specific consortium of microorganisms that allows high recovery of said metals. The consortium is referred to as "Kobold B(S)" (DMS 35389) and is made up of three layers which belong to three genera of microorganisms: Sulfobacillus, Acidithiobacillus and Leptospirillum.

The invention discloses extreme acidithiobacillus acidophilus based on sulfur metabolic pathway enhancement as well as a construction method and application of the extreme acidithiobacillus acidophilus. According to the invention, based on conjugational transfer, sulfide of Acidithiobacillus caldus, namely a quinone oxidoreductase SQR gene is over-expressed, a promoter Amp is adopted for starting expression, an extreme acidophilic engineering bacterium A. caldus-G-SQR is constructed, and compared with wild type A. caldus, the acid stress tolerance, sulfur oxidation capacity and intracellular ATP (adenosine triphosphate) generation capacity of the extreme acidophilic engineering bacterium A. caldus-G-SQR are all improved. The core effect of the SQR in strain environmental adaptation and metabolic regulation is fully verified.

The application provides an extreme acidophilic sulfur-oxidizing bacterium and application thereof. The extreme acidophilic bacterium has strong oxidation on elemental sulfur and tetrathionate ions, is a chemolithoautotrophic acidithiobacillus, and can be used for removing elemental sulfur and tetrathionate ions in the environment, and has wide application prospects. The acidophilic sulfur-oxidizing bacterium has a preservation number of CGMCC No. 14839. The optimal growth temperature of the biological leaching and desulfurization provided by the application is normal temperature, and the optimal growth pH range is wide, so that the strain of the application has practical application significance. In addition, due to the characteristics of chemolithoautotrophy, the strain can improve the climate environment while recovering the environment.

The present invention discloses a method for biosynthesizing cadmium sulfide quantum dots using the culture supernatant of Acidithiobacillus. The method comprises the following steps: using the supernatant of Acidithiobacillus cultured to a stable phase as a catalytic reaction system, adjusting its pH to neutral, adding a sulfur source and a cadmium source, and culturing on a shaking table to obtain cadmium sulfide quantum dots; the S source is a sulfur-containing amino acid, the Cd source is a soluble cadmium salt, and the Acidithiobacillus is a member of the genus Thiobacillus that uses inorganic sulfur as an energy source. The present invention utilizes the unique sulfur metabolism mechanism of Acidithiobacillus and can achieve the biocatalytic synthesis of a large number of water-soluble cadmium sulfide quantum dots using only the supernatant of Acidithiobacillus. Compared with other reported biosynthesis methods, the present invention has significant advantages in that it does not require the use of bacterial cells or purified proteases, is simple to operate, has mild preparation conditions, and is low in cost.

The invention relates to the technical field of biological treatment of solid wastes, and discloses thiobacillus thiooxidans resistant to toxicity of Ni and Cu and application of the thiobacillus thiooxidans. Wherein the thiobacillus thiooxidans is the thiobacillus thiooxidans QH2C, and the preservation number of the thiobacillus thiooxidans is GDMCC No: 63521. The invention further discloses a preparation method of the thiobacillus thiooxidans. The invention provides a novel thiobacillus thiooxidans QH2C, and the strain has high toxicity tolerance to Ni and Cu, so that the strain can be used for treating metal-containing solid wastes with higher ore pulp density in batches, the biological leaching treatment efficiency is improved, and the application prospect is good.