The invention belongs to the field of natural organic chemistry and relates to a method for extracting and purifying total alkaloids from wild thermopsis lanceolata in Greater Khingan Mountains and particularly relates to a method for finally obtaining high-purity total alkaloids by using a biological enzymolysis technology and through ultrahigh pressure coupled subcritical water extraction, separation and refining by using an enzymatic method as well as separation and purification by using a high-speed counter-current chromatography. The method has the advantages that 1, the effective components of the alkaloids are rapidly dissolved out and the high activity is kept by using a biological exine detaching technology, and the method is carried out at normal temperature and is low in energy consumption and capable of realizing industrial production; 2, the subcritical water is nontoxic and pollution-free, so that the activity of an extract is not damaged; the operation cost is low, the extract is easy to be separated from a product, and large-scale industrial production can be realized; 3, the high-speed counter-current chromatography has the advantages of flexibility in operation, high efficiency, high separation speed, large preparation amount, low expense and the like, and can be used for industrial application. By using the method, the obtained total alkaloids are high in content and high in purity up to 95.8%.