A method for secondary immobilization of Debaria hansenii by using composite materials

A composite material and yeast technology, applied in the direction of immobilized enzymes, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of overcoming low yield, novel preparation method, and high yield

Active Publication Date: 2020-08-18
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems to be solved in immobilized cell technology, such as immobilization methods, finding embedding materials with strong tolerance, improving product yield, etc.

Method used

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  • A method for secondary immobilization of Debaria hansenii by using composite materials
  • A method for secondary immobilization of Debaria hansenii by using composite materials
  • A method for secondary immobilization of Debaria hansenii by using composite materials

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Debaria hansenii immobilized by sodium alginate and activated carbon

[0031] Use the seed slant medium to activate Debaria hansenii, obtain the Debaria hansenii liquid fermentation strain after culturing in the seed medium and fermentation medium, filter the sterile gauze to separate the Debaria hansenii cells and ferment culture Base, according to the ratio of 0.5:1 (w / v), the liquid fermentation strain of Debaria hansenii was mixed with sodium alginate and activated carbon, the final concentration of sodium alginate was 25g / L, and the final concentration of activated carbon was 8g / L; Use a syringe to draw the mixture and drop it into CaCl 2 solution, the CaCl 2 The concentration of the solution was 19g / L, fixed at room temperature for 1h, and prepared into immobilized Debaryomyces hansenii pellets with a diameter of 0.3cm. After the pellets were separated by filtration with sterile gauze, they were washed twice with sterile water;

[0032] (2) Secondary immobil...

Embodiment 2

[0037] (1) Debaria hansenii immobilized by sodium alginate and activated carbon

[0038] Use the seed slant medium to activate Debaria hansenii, obtain the Debaria hansenii liquid fermentation strain after culturing in the seed medium and fermentation medium, filter the sterile gauze to separate the Debaria hansenii cells and ferment culture Base, according to the ratio of 1:1 (w / v), the liquid fermentation strain of Debaria hansenii was mixed with sodium alginate and activated carbon, the final concentration of sodium alginate was 30g / L, and the final concentration of activated carbon was 9g / L; Use a syringe to draw the mixture and drop it into CaCl 2 solution, the CaCl 2 The concentration of the solution was 20 g / L, fixed at room temperature for 2 hours, and prepared into immobilized Debaria hansenii pellets with a diameter of 0.4 cm. After the pellets were separated by filtration with sterile gauze, they were washed twice with sterile water;

[0039] (2) Secondary immobil...

Embodiment 3

[0044] (1) Debaria hansenii immobilized by sodium alginate and activated carbon

[0045] Use the seed slant medium to activate Debaria hansenii, obtain the Debaria hansenii liquid fermentation strain after culturing in the seed medium and fermentation medium, filter the sterile gauze to separate the Debaria hansenii cells and ferment culture According to the ratio of 1:1 (w / v), the liquid fermentation strain of Debaria hansenii was mixed with sodium alginate and activated carbon, the final concentration of sodium alginate was 35g / L, and the final concentration of activated carbon was 10g / L; Use a syringe to draw the mixture and drop it into CaCl 2 solution, the CaCl 2 The concentration of the solution was 21g / L, fixed at room temperature for 3h, prepared into immobilized Debaria hansenii pellets with a diameter of 0.5cm, and washed 2 times with sterile water after the pellets were separated by filtration with sterile gauze;

[0046] (2) Secondary immobilization of Debaria ha...

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Abstract

The invention discloses a method for secondary immobilization of Debaryomyces hansenii with a composite material. The method includes the steps of: employing sodium alginate and activated carbon to immobilize Debaryomyces hansenii; using polyvinyl alcohol and sodium alginate to perform secondary immobilization of Debaryomyces hansenii; and placing the Debaryomyces hansenii subjected to secondary immobilization in a fermentation medium for culture. The preparation method provided by the invention has the characteristics of novelty, simplicity, easily controllable operation program, and high yield, and overcomes the disadvantage of low 3-hydracrylic acid yield of traditional strains.

Description

technical field [0001] The invention relates to a method for immobilizing Debaria hansenii, in particular to a method for secondary immobilization of Debaria hansenii by using a composite material. Background technique [0002] 3-hydroxypropionic acid is mainly used as a chemical intermediate in industry, and can synthesize vinyl cyanide, methyl acrylate, ethyl acrylate, 1,3-propanediol and malonic acid, etc. It is also a new type of green environmental protection material poly 3-hydroxypropane An important raw material for acids. In addition, 3-hydroxypropionic acid is widely used in the production of special coatings, metal lubricants, antistatic agents in the textile industry, food, feed additives and fresh-keeping agents. [0003] At present, the production of 3-hydroxypropionic acid by chemical methods has been gradually replaced by biosynthetic methods due to the greater harm to environmental pollution. However, the production of 3-hydroxypropionic acid by biosynthes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/084C12N11/04C12R1/645
CPCC12N11/04C12N11/08C12N11/10C12N11/14
Inventor 董玉玮高明侠王陶张传丽
Owner XUZHOU UNIV OF TECH
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