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This invention discloses a method for preparing cubic nano-calcium carbonate based on monosaccharide regulation. Using monosaccharides as crystal guiding agents, the resulting cubic nano-calcium carbonate exhibits a cubic structure with an average particle size of 81-115 nm. The monosaccharide is either glucose or fructose. The preparation method includes the following steps: 1. Preparation of calcium oxide; 2. Preparation of monosaccharide-nano-calcium carbonate. The amount of monosaccharide added is 0.8-1.2 wt.% of the calcium oxide mass; the calcium oxide yield is 65-75%. The resulting nano-calcium carbonate has a whiteness value of 90.6-97.3 wb and an oil absorption value of 65.3-89.6 g DOP / 100 g CaCO3.