67results about How to "Improve sugar conversion rate" patented technology

The invention provides a method for producing L-ornithine by microbial fermentation, namely, using Corynebacterium glutamate to obtain the strains of CS-189(cit<(-)>+SG<r>) by chemical treatment, and obtain the L-ornithine hydrochloride by fermentation, culture solution micro-filtration by a ceramic membrane, ion exchange resin and extraction by a hydrothermal crystallization method with decompression concentration. The strains used in the method are auxotrophy resistant to sulfaguanidine, which significantly enhances acid yield by fermentation. Meanwhile, aiming at the problem that the solubility of the L-ornithine hydrochloride in water is extremely difficult, the hydrothermal crystallization method is adopted to obtain better extraction rate under the condition that flammable and explosive alcohol is not used. The method simplifies processes and reduces extraction cost.

The invention belongs to the technical field of biological engineering, which relates to a method for separating and extracting boletic acid in situ in the process of utilizing rhizopus oryzae fermentation to prepare boletic acid. The invention aims at the special physicochemical properties of boletic acid, when rhizopus oryzae free cell is utilized to ferment, the invention adopts a hollow fiber ultrafilter membrane, concentration by nanofiltration and ion exchange adsorption coupling technique to separate and extract boletic acid. The operation condition of the method is mild, separation selectivity is strong, activity loss of somatic cell is small, fermentation efficiency of rhizopus oryzae is effectively improved, process flow of utilizing a biological method to synthesize boletic acid is reduced, and meanwhile, the invention utilizes a nanofiltration device to concentrate fermentation liquor in the process of reacting and separating, which greatly improves adsorption capacity of ion exchange resin to boletic acid, and material consumption and energy consumption are relatively lower, and waste and secondary pollutants which are produced are relative little. The reacting and separating process of the method are fast, complete, and operation stability is excellent, the method is a high-effective and novel mass transfer separation coupling strengthening technique, which has wider industrialized production prospect.

The invention relates to a method for producing ethanol by continuous enzymolysis and fermentation of lignocelluloses. The method comprises the following steps: (1) a pretreated lignocellulose raw material and an enzyme solution are added in proportion into an enzymolysis reactor with a screw device to carry out continuous enzymolysis; (2) the materials enter a fermentation tank I after enzymolysis, and microzyme is inoculated to carry out simultaneous saccharification and fermentation at 30-38 DEG C; (3) the materials in the fermentation tank I enter a fermentation tank II, and simultaneous saccharification and fermentation are continuously carried out at 39-44 DEG C; (4) the fermented materials undergo reduced pressure distillation; and (5) the distilled materials undergo solid-liquid separation, and an enzyme-containing liquid phase is reused in the step (1). By the use of the screw device, continuous supply and continuous enzymolysis of the raw materials are realized, and enzymolysis efficiency is enhanced. As simultaneous saccharification and fermentation are carried out at different temperatures, enzymolysis efficiency is further improved and yield of ethanol is then raised. The finally-obtained enzyme solution can be directly reused in the enzymolysis process, thus reducing production cost.

The invention relates to a pre-treating method for improving the sugar conversion rate of lignocellulose. The pre-treating method comprises the following steps: (1) removing impurities in a lignocellulose raw material, and carrying out air drying, smashing and sieving on the lignocellulose to prepare a cellulose raw material; (2) adding the cellulose raw material into a NaOH solution, uniformly stirring, then sequentially adding in H2O2 and cyanogen amine adopted as an activator, uniformly stirring again to prepare a mixed reaction liquid, and reacting by stirring to prepare the pre-treated lignocellulose. The pre-treating method adopts the method of combining low-concentration NaOH and H2O2 with the activator to treat the cellulose raw material, the pre-treating process is simple and feasible, intractable wastewater is prevented from generating, and the sugar conversion rate of the lignocellulose is remarkably improved.

The invention provides a method for producing fermentable sugar through the zymohydrolysis of kenaf bast fiber, in particular the invention provides a method for preparing sugar through the degradation of cellulose, which comprises the following steps: physical degumming, steam blasting and zymohydrolysis are conducted to the kenaf bast fiber, so as to produce the sugar. The fermentable sugar produced by the method can be used for the production of fuel ethanol, and is characterized by short treatment time, high conversion rate, light pollution and the like.

The present invention discloses a guanyin tea preparation method comprising the following steps: picking Tieguanyin fresh leaves; grading the raw material for fine selection; spreading for cool; sunning of the green leaf; rocking of the green leaf; rolling; fermenting; first stir-frying for fixing; second stir-frying for fixing; packed rolling; deblocking to remove powder; passing through fire; packed rolling for forming; roasting to dry enough; sieving sorting and packaging. The guanyin tea preparation method is improved based on the traditional Tieguanyin tea preparation process, in the whole process, traditional Chinese medicine liquid spraying treatment is performed, the self flavor of guanyin tea is retained, the guanyin tea has the effects of clearing heat and cooling blood, purging fire for removing toxin, lowering blood pressure, benefitting qi and blood, and improving the body's autoimmunity, the traditional Chinese medicine liquid also contains beta-glucosidase, the sugar conversion rate is improved, compared with tea without enzyme treatment, the obtained Tieguanyin tea is more smooth and slippery in mouthfeel, tastes more sweet, is faster in sweetness after taste, can effectively improve the Tieguanyin tea quality, and further increases the economic benefits of the tea.

The invention relates to cladosporium cladosporioides preserved at the China General Microbiological Culture Collection Center on September 4, 2014, wherein the preservation number is CGMCC NO.9587. The invention further provides an enzyme preparation containing the cladosporium cladosporioides and application of the enzyme preparation in saccharification of a lignocelluloses raw material. The lignocellulolyticenzyme preparation provided by the invention is capable of finishing the saccharification treatment process of the lignocelluloses raw material at a relatively low temperature, is high in polysaccharide yield, low in energy consumption, especially suitable for large-scale industrial application in the field of cellulosic ethanol fermentation production; and the raw materials are relatively fully utilized.

The invention provides a processing method for sweet Tie Guanyin tea. According to the processing method, cellulase liquids with different concentrations are spayed between a cooling step and a rocking step in the conventional production process of the Tie Guanyin tea. The processing method comprises the following specific steps: carrying out primary cooling on the sunned tea, spraying the cellulase liquids on the primarily cooled tea and then carrying out primary rocking; after the primary rocking is ended, carrying out secondary cooling, spraying the cellulase liquids on the secondarily cooled tea and then carrying out secondary rocking; after the secondary rocking is ended, carrying out cooling for the third time, spraying the cellulase liquids on the cooled tea for the third time and then carrying out rocking for the third time; after the rocking for the third time is ended, carrying out cooling for the fourth time. Compared with the tea not treated by the cellulase liquids, the Tie Guanyin tea produced by the processing method provided by the invention has the advantages of smoother mouthfeel, sweeter taste and higher sweet aftertaste speed; the quality of the Tie Guanyin tea is effectively improved; economic benefits of the tea are increased.

The invention relates to a method for preparing ethanol by adopting materials containing cellulose, which comprises the following steps: the materials containing cellulose is exploded by steam; the obtained steam explosion products are mixed with enzyme for enzymolysis; the enzymolysis products are obtained by fermentation; wherein, the method for mixing the steam explosion products and the enzyme comprises the following steps: a first batch of steam explosion products is firstly mixed with the enzyme to obtain mixture, and then the rest steam explosion products are continuously added into the mixture to be mixed with the obtained mixture; wherein, the adding speed of the rest steam explosion products ensures the concentration of the steam explosion products in the reaction system not to be higher than 150g/L; the weight of the first batch of steam explosion products mixed with the enzyme is 10-30wt% of the whole steam explosion products; and the liquid water content of the materials containing cellulose is 30-60 wt%. The method can effectively improve the saccharous conversion rate of the materials and the productivity of the ethanol, can greatly shorten the enzymolysis time and improve the enzymolysis efficiency as well.

The invention discloses a method for preparing ethanol from a cellulose-containing raw material, which comprises the following steps: in the presence of water, mixing a cellulose-containing raw material with enzyme, carrying out enzymolysis on the cellulose-containing raw material to obtain an enzymolysis product, and fermenting the enzymolysis product. The enzymolysis comprises a main enzymolysis stage and an intermitted enzymolysis stage after the main enzymolysis stage which are continuously performed, wherein in the main enzymolysis stage, proper amounts of the cellulose-containing raw material and enzyme are continuously added into an enzymolysis tank so that the monosaccharide content in the enzymolysis product in the main enzymolysis stage is 50-100 g/L, and the enzymolysis products of the main enzymolysis stage respectively and continuously flow into enzymolysis tanks of the subsequent stage to perform intermitted enzymolysis until the monosaccharide content in the enzymolysis products in the enzymolysis tanks in the subsequent stage at the enzymolysis end point is greater than or equal to 105 g/L. The method can effectively enhance the saccharide conversion rate of the raw material, and therefore, greatly enhances the yield of ethanol.

The invention discloses a Sphingomonas paucimobilis strain and application thereof and belongs to the technical field of bioengineering. The Sphingomonas paucimobilis strain is named Sphingomonas paucimobilis strain Y5, is collected in CCTCC (China Center for Type Culture Collection), which is located in Wuhan University, Wuhan, China, and has the collection number of CCTCC NO: M 2015523. Welan gum can be prepared from the train by fermenting under appropriate conditions through carbon sources, nitrogen sources, inorganic salts and water . The strain disclosed by the invention has relatively high sugar conversion ratio and welan gum yield and has a certain application value. The welan gum produced through fermenting the strain is high in quality and is resistant to acids and bases, a 1% aqueous solution viscosity of the welan gum reaches 3500mPa.S, and the viscosity is fundamentally unchanged at the temperature of 150 DEG C. During the fermentation of the strain, the sugar conversion ratio is relatively high and reaches 55% to 60%, and the yield is relatively high and reaches 25-32g/L (dry weight).

The invention discloses health yellow wine for lowering blood sugar and blood fat and a preparation method of the health yellow wine. The health yellow wine is prepared from the following raw materials in parts by weight: 40-60 parts of glutinous rice flour, 2-3 parts of edible salt, 10-15 parts of corn kernels, 10-15 parts of buckwheat grains, 6-9 parts of cucumber, 4-6 parts of dark tea, 4-6 parts of sargassum fusiforme, 4-6 parts of Lycium ruthenicum, 2-3 parts of radix puerariae, 4-6 parts of leaves of cyclocarya paliurus, 3-5 parts of basil seeds, 10-15 parts of acid soluble soy protein,a proper amount of alpha-amylase and complex enzyme and a proper amount of glucoamylase and yellow wine dry yeast. Various natural raw materials with functions of reducing blood sugar and reducing blood fat are selected and improvements are made on the basis of the traditional yellow wine production process, effective components of the raw materials can be fully retained, and nutritional and health value of the yellow wine is improved; the finished yellow wine is clear in body, soft in taste, unique in flavor, fragrant in bouquet, obvious in fragrance retention and high in stability.

The invention discloses a method for preparing ethanol by using a raw material containing cellulose, which comprises the steps of subjecting the raw material containing cellulose to steam explosion, mixing an obtained product of the steam explosion with an enzyme for enzymolysis, and fermenting the obtained enzymatic product, wherein a method for the steam explosion of the raw material containing cellulose comprises the steps of continuously delivering the raw material containing cellulose to a heat-preservation pressure unit, keeping the raw material containing cellulose in the heat-preservation pressure unit for 3 to 10 minutes and then releasing part of the raw material containing cellulose in the heat-preservation pressure unit to normal pressure every other 5 to 12 seconds; and a method for mixing the product of the steam explosion and the enzyme comprises the step of mixing the product of the steam explosion with the enzyme in batches, wherein the batches are at least two batches, and in each batch, the additive amount of the product of the steam explosion accounts for 60 percent of the total weight of the product of explosion at most. The method of the invention can effectively improve the sugar conversion rate of the raw material, thereby greatly improving ethanol yield.

The invention provides a double-body circulatory cellulose enzymatic hydrolysis reactor and an application of the double-body circulatory cellulose enzymatic hydrolysis reactor. The double-body circulatory cellulose enzymatic hydrolysis reactor specifically comprises a first reaction tank, a second reaction tank, a first pipe and a second pipe. The first reaction tank comprises a first reaction tank body, a shearing homogenizer, an inlet and an outlet, wherein the shearing homogenizer is arranged in the first reaction tank body, the inlet is used for feeding, and the outlet is used for discharging; the second reaction tank comprises more than two reaction pipes which are connected in series or parallel; the first pipe is used for connecting the first reaction tank with the second reaction tank; and the second pipe is used for connecting the reaction pipes in the second reaction tank. When being used for the enzymolysis and the saccharification of the cellulose such as the hemp bastuse, the double-body circulatory cellulos enzymatic hydrolysis reactor has the advantages of high conversion rate, high concentration, short conversion time and low enzyme preparation consumption, etc.

The invention discloses a method for preparing monosaccharide by using a raw material containing cellulose, which comprises the steps of subjecting the raw material containing cellulose to steam explosion and mixing an obtained product of the steam explosion with an enzyme for enzymolysis, wherein a method for the steam explosion of the raw material containing cellulose comprises the steps of continuously delivering the raw material containing cellulose to a heat-preservation pressure unit, keeping the raw material containing cellulose in the heat-preservation pressure unit for 3 to 10 minutes first and then releasing part of the raw material containing cellulose in the heat-preservation pressure unit to normal pressure every other 5 to 12 seconds; and a method for mixing the product of the steam explosion and the enzyme comprises the step of mixing the product of the steam explosion with the enzyme in batches, wherein the batches are at least two batches, and in each batch, the additive amount of the product of the steam explosion accounts for 60 percent of the total weight of the product of explosion at most. The method can improve the monosaccharide yield effectively.

A method which uses cellulose-containing raw materials for preparing monosaccharide comprises the steps as follows: the raw materials which contain cellulose is carried out steam blasting; a product which is obtained by steam blasting is mixed with enzyme and then experiences enzymolysis, wherein, the method which leads the steam blasting product to be mixed with the enzyme comprises: firstly, a first batch of steam blasting products are mixed with the enzyme for obtaining a mixture; and then the remaining steam blasting products are continuously added to be mixed with the mixture; the speed for adding the remaining steam blasting products causes the concentration of the steam blasting products in a reaction system not to be higher than 150 grams/liter; the weight of the first batch of steam blasting products which are mixed with the enzyme is10-30% of the total steam blasting products; the water content of the raw materials which contain cellulose is 30-60%. The method can effectively improve the sugar conversion rate of raw materials, namely the yield of monosaccharide; in addition, the method of continuously supplementing materials can also significantly shorten the enzymolysis time and improve the enzymolysis efficiency.

The invention provides a high-capacity double-systematic-circulation cellulose enzymolysis reactor and its application. The high-capacity double-systematic-circulation cellulose enzymolysis reactor concretely comprises: a first reaction tank (a) comprising a tank body, shearing homogenizers positioned in the tank body, an inlet used for adding a feed material, and an outlet used for discharging the material; a second reaction tank (b) comprising above two series or parallel reaction tubes; a pipeline (c) connecting the first reaction tank with the second reaction tank; and a pipeline (d) connecting all tubes in the second reaction tank, wherein the number of the shearing homogenizers is two or more; and the volume of the first reaction tank is 3-150t, and preferably 30-100t. The enzymolysis reactor has the advantages of high conversion rate, high concentration, short conversion time, low enzyme preparation consumption, low energy consumption and the like when the enzymolysis saccharification of celluloses comprising fibrilia and the like is carried out by the enzymolysis reactor, so the enzymolysis reactor is especially suitable for high-capacity cellulose enzymolysis reaction systems.

The invention provides a method for preparing a carbon source for biological denitrification by utilizing sugary waste liquid. The method provided by the invention comprises three steps of preparation of a biological carrier, fixation of saccharomyces cerevisiae and preparation of the carbon source for biological denitrification; the biological carrier adopted by the method is orange peel, and abundant nutritious substances of the orange peel enable the saccharomyces cerevisiae fixed thereon to be propagated when carbohydrate is fermented, thereby increasing the fermentation speed; the conversion rate of sugar is high; in addition, the orange peel is degraded in a fermentation process, thereby increasing the quantity of fermentation products; by utilizing the saccharomyces cerevisiae to ferment sugaring waste, the carbohydrate in the waste is converted into low-molecule alcohol and organic acid; compared with a way of directly using the sugaring waste as the denitrification carbon source, the effective component content is high, and the problems of few effective substances, more foam and the like occuring when the carbohydrate is directly used as the carbon source can be avoided; according to the method, high-valued application of the sugaring waste and the orange peel can be achieved, and the conversion of the carbohydrate to the low-molecule alcohol and organic acid during storage and transportation of a product is realized.

The present invention discloses a coryneform CGMCC NO.0785 and a process for preparing L-lysine by its fermentation. Said process includes such steps as culturing coryneform CGMCC NO.0785, fermenting in culture medium, and collecting L-lysine from its fermented liquid. Its advantages are high output rate, high conversion rate of saccharide and low cost.

The invention relates to an escherichia coli fermentation medium and a fermentation technology for producing succinic acid. A formula of the medium comprises 1.4-1.6g/dL corn steep liquor dry powder,0.5-2.5g/L yeast extract, 0.2-1.4g/L MgSO4, 2.5-4.5g/L (NH4)2HPO4, 0.6-2.0g/L C5H11NO2.HCl, 0.2-1.0g/L KCl, 50-60mg/L CuSO4, and 35-50mg/L FeSO4.7H2O; a pH of the medium is 6.5-6.8; and a required volume is reached by distilled water. The fermentation technology comprises the steps of taking escherichia coli as a fermentation strain, and performing aerobic enrichment in a primary seeding tank anda secondary seeding tank, and anaerobic fermentation in a fermentation tank to form succinic acid, wherein a formula of the fermentation medium is the above formula. According to the medium and the technology, the total fermentation cost can be lowered by 22%.