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Double-body circulatory cellulose enzymatic hydrolysis reactor and application thereof

A technology of cellulase and reactor, which is applied in the direction of enzyme production/bioreactor, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., which can solve the problem of low enzyme consumption and difficult enzymatic hydrolysis process , time-consuming and other issues

Active Publication Date: 2012-07-25
SHANGHAI ZHONGWEI BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the crystallinity, polymerization degree and tendency of hemp bast fiber are much higher than those of other biomass (such as starch), the enzymatic hydrolysis process with glucose as the conversion target (or other monomers) is quite difficult
At present, there is no satisfactory, efficient, short time-consuming and low enzyme consumption equipment and process for enzymatic hydrolysis and saccharification of hemp bast fiber

Method used

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  • Double-body circulatory cellulose enzymatic hydrolysis reactor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] enzymatic saccharification

[0104] use figure 1 The enzymatic saccharification device shown performs enzymatic saccharification according to the following steps:

[0105] 1. Steps:

[0106] 1.1 The slurry solution after pretreatment is prepared into a 100L solution with a substrate concentration of 6%.

[0107] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution, and adjust the pH value to 4.8.

[0108] 1.3 Heat the jacket of the two-body cycle enzymolysis reactor to 50°C;

[0109] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. Wherein the total consumption of cellulase (including endoglucanase, exoglucanase and β-glucosidase) is 1000IU / ml. The slurry solution is poured into the first reaction tank at a speed of 600rpm, and from the first reaction tank into the second reaction tank at a speed of 50rp...

Embodiment 2

[0119] use figure 1 The enzymatic saccharification device shown performs enzymatic saccharification according to the following steps:

[0120] 1. Steps:

[0121] 1.1 The slurry solution after pretreatment is prepared into a 100L solution with a substrate concentration of 6%.

[0122] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution, and adjust the pH value to 4.8.

[0123] 1.3 Heat the jacket of the two-body cycle enzymolysis reactor to 50°C;

[0124] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. Wherein the total consumption of cellulase (including endoglucanase, exoglucanase and β-glucosidase) is 900IU / ml. The slurry solution is poured into the first reaction tank with a rotating speed of 1500rpm, and from the first reaction tank into the second reaction tank with a rotating speed of 120rpm. All the ma...

Embodiment 3

[0134] use figure 1 The enzymatic saccharification device shown performs enzymatic saccharification according to the following steps:

[0135] 1. Steps:

[0136] 1.1 The slurry solution after pretreatment is prepared into a 100L solution with a substrate concentration of 6%.

[0137] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution, and adjust the pH value to 4.8.

[0138] 1.3 Heat the jacket of the two-body cycle enzymolysis reactor to 50°C;

[0139] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. Wherein cellulase is acid cellulase, and the total consumption is 1200IU / ml. The slurry solution is poured into the first reaction tank at a speed of 2800rpm, from the first reaction tank into the second reaction tank at a speed of 250rpm. All the materials carry out two-body circulation between the first reacti...

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Abstract

The invention provides a double-body circulatory cellulose enzymatic hydrolysis reactor and an application of the double-body circulatory cellulose enzymatic hydrolysis reactor. The double-body circulatory cellulose enzymatic hydrolysis reactor specifically comprises a first reaction tank, a second reaction tank, a first pipe and a second pipe. The first reaction tank comprises a first reaction tank body, a shearing homogenizer, an inlet and an outlet, wherein the shearing homogenizer is arranged in the first reaction tank body, the inlet is used for feeding, and the outlet is used for discharging; the second reaction tank comprises more than two reaction pipes which are connected in series or parallel; the first pipe is used for connecting the first reaction tank with the second reaction tank; and the second pipe is used for connecting the reaction pipes in the second reaction tank. When being used for the enzymolysis and the saccharification of the cellulose such as the hemp bastuse, the double-body circulatory cellulos enzymatic hydrolysis reactor has the advantages of high conversion rate, high concentration, short conversion time and low enzyme preparation consumption, etc.

Description

technical field [0001] The invention relates to the field of new energy, more specifically, to the field of enzymatic saccharification using plant cellulose as raw material. The invention provides a high-efficiency enzymolysis double-body circulation cellulose enzymolysis reactor and an application thereof using bast fiber of hemp crops as a raw material. Background technique [0002] At present, human beings have a great demand for energy, but resources such as petroleum are non-renewable resources, so people vigorously develop various new energy sources. [0003] Ethanol can be burned alone, and can also be used as a good gasoline oxygenate and high-octane blending component. It has been recognized as one of the most promising renewable clean energy sources. Its production and application have shown a high-speed development trend in the world. The fuel ethanol industry has become an important policy means and economic lever for governments to solve the shortage of oil res...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/02C12P19/14
CPCC12M29/18C12M23/06C12M27/06C12M23/58C12M45/02C12M21/18C12M27/02C12M45/09
Inventor 张士楚朱震黎王恒新王荣华
Owner SHANGHAI ZHONGWEI BIOCHEM
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