Double-body circulatory cellulose enzymatic hydrolysis reactor and application thereof
A technology of cellulase and reactor, which is applied in the direction of enzyme production/bioreactor, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., which can solve the problem of low enzyme consumption and difficult enzymatic hydrolysis process , time-consuming and other issues
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[0102] Example 1
[0103] Enzymatic saccharification
[0104] use figure 1 The enzymatic hydrolysis and saccharification device shown in the following steps:
[0105] 1. Steps:
[0106] 1.1 The pretreated slurry solution is prepared into a 100L solution with a substrate concentration of 6%.
[0107] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution and adjust the pH to 4.8.
[0108] 1.3 Heat the jacket of the double-body circulation enzymatic hydrolysis reactor to 50°C;
[0109] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. The total dosage of cellulase (including endoglucanase, exoglucanase and β-glucosidase) is 1000IU / ml. The slurry solution was driven into the first reaction tank with a rotation speed of 600 rpm, and from the first reaction tank into the second reaction tank, the rotation speed of the second reactio...
Example Embodiment
[0118] Example 2
[0119] use figure 1 The enzymatic hydrolysis and saccharification device shown in the following steps:
[0120] 1. Steps:
[0121] 1.1 The pretreated slurry solution is prepared into a 100L solution with a substrate concentration of 6%.
[0122] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution and adjust the pH to 4.8.
[0123] 1.3 Heat the jacket of the double-body circulation enzymatic hydrolysis reactor to 50°C;
[0124] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. The total dosage of cellulase (including endoglucanase, exoglucanase and β-glucosidase) is 900IU / ml. The slurry solution was driven into the first reaction tank with a rotation speed of 1500 rpm, and from the first reaction tank into the second reaction tank, the rotation speed of the second reaction tank was 120 rpm. All materials ...
Example Embodiment
[0133] Example 3
[0134] use figure 1 The enzymatic hydrolysis and saccharification device shown in the following steps:
[0135] 1. Steps:
[0136] 1.1 The pretreated slurry solution is prepared into a 100L solution with a substrate concentration of 6%.
[0137] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution and adjust the pH to 4.8.
[0138] 1.3 Heat the jacket of the double-body circulation enzymatic hydrolysis reactor to 50°C;
[0139] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. The cellulase is acid cellulase, and the total dosage is 1200IU / ml. The slurry solution was driven into the first reaction tank with a rotation speed of 2800 rpm, and from the first reaction tank into the second reaction tank, the rotation speed of the second reaction tank was 250 rpm. All materials undergo a double-body circulation...
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