34results about How to "Improve enzymatic hydrolysis and saccharification efficiency" patented technology

The invention discloses a pretreatment method for improving enzymatic hydrolysis and saccharification efficiency of a non-wood fiber raw material. The method comprises the following steps of: mixing an aqueous solution of sodium carbonate and the non-wood fiber raw material, pre-impregnating for 30 to 120 minutes at the temperature of between 30 and 90 DEG C, heating to the temperature of between 100 and 180 DEG C, performing heat preservation reaction for 0 to 120 minutes, washing the non-wood fiber raw material by using water, defibering the raw material into fibrous pulp by using a stuff grinder, and thus completing the pretreatment process. The yield of the pretreated non-wood fiber raw material is between 50 and 90 percent, the removal rate of lignin is between 10 and 80 percent, the loss of high glycan is between 0 and 25 percent, a little silicon dioxide is dissolved from the non-wood fiber raw material in the pretreatment solution, and most silicon dioxide is preserved in the pretreated non-wood fiber raw material.

The invention discloses a pretreatment method capable of improving saccharification efficiency of lignocellulose. According to the method, ethanedioic acid, choline chloride and glycerol are added towater for reaction to obtain a modified deep-eutectic solvent (DES), and a lignocellulose raw material is pretreated with the modified DES. The method can realize pretreatment of the lignocellulose under a lower-temperature condition, is more beneficial to improvement of the saccharification efficiency of the lignocellulose and can substantially increase the total quantity of carbohydrates released in a lignocellulose hydrolytic process.

The invention provides a combined pretreatment process for improving the lignocellulose saccharification effect. According to the process, firstly, an alkali/urea solution is used for conducting first-step treatment on lignocellulose raw materials, part of lignin and hemicellulose ingredients in the lignocellulose raw materials are removed through chemical treatment, and the stable structure of lignocellulose is damaged to a certain degree; physical treatment is further conducted through ultrasonic waves, and the structure of the lignocellulose is more loosened. After pretreatment of the process, the ingredient content of the lignocellulose is obviously changed, and the proportion of cellulose is remarkably increased. In addition, the structure of the lignocellulose is remarkably changed, the raw material surface becomes rough, loosened and porous, and the accessible surface in the enzymolysis saccharification process is greatly increased. The combined pretreatment process has the advantages of being not high in equipment requirement, simple in operation, moderate in treatment condition, low in cost and the like, and can greatly improve the saccharification efficiency of the lignocellulose.

The invention relates to a method for improving the enzymolysis saccharifying efficiency of hemp straw. The method is mainly characterized in that before enzymolysis saccharifying of hemp straw, the following pretreatment is performed: (1) crushing hemp straw, performing high-temperature sterilization, then inoculating white rot fungi into the crushed straw, and performing culture for 7-28 days toobtain biologically pre-treated hemp straw; and (2) mixing the biologically pre-treated hemp straw with a low-concentration alkali and hydrogen peroxide mixed solution, and performing treatment for 20-30 h at 40-60 DEG C to obtain pre-treated hemp straw, wherein the mass concentration of alkali in the mixed solution is 0.5-5%, and the volume concentration of hydrogen peroxide is 2.5-3.5%. Beforealkali oxygen treatment of hemp, biological pretreatment is performed at first, so that the consumption and the concentration of alkali in the alkali oxygen treatment process are reduced, the treatment time is shortened, the treatment efficiency is improved, and the pretreatment effect is improved.

The invention relates to a process for improving enzymolysis saccharification efficiency of lignocelluloses through combined pretreatment of an NADES and NU. The process comprises the following steps: firstly, mixing choline chloride/lactic acid according to a certain ratio, and performing further treatment on a lignocelluloses raw material so as to remove a part of lignin and hemicelluloses and damage the dense structure of the lignocelluloses; further performing a second step of pretreatment on the lignocelluloses by using NU at different temperature. By adopting the process, the ratios of the lignin and the hemicelluloses can be reduced, the surfaces of the lignocelluloses are loosened and porous, the accessible areas of the lignocelluloses are increased, and the saccharification efficiency of the lignocelluloses is improved. The combined pretreatment process has the advantages of being low in equipment requirement, simple to operate, gentle in treatment condition, low in cost, environmentally friendly and the like, and the saccharification efficiency of the lignocelluloses can be greatly improved.

The invention discloses a biological-chemical combined treatment process for improving saccharifying effect of lignocellulose. According to the process, a lignocellulose raw material is subjected to biological treatment by virtue of an efficient lignocelluloses degrading bacterium sphingobacterium sp. LD-1 (preservation number is CGMCC No.10920), so as to remove partial lignin and hemicellulose components in the lignocellulose raw material with the action of microorganisms, and to damage the stable structure of the lignocellulose to a certain degree; and furthermore, the lignocellulose undergoes chemical treatment at low temperature by virtue of alkali/urea, so that the lignocellulose is more loose in structure. The lignocellulose, which is preprocessed by the process disclosed by the invention, achieves significant change in component content and obvious increase in proportion of cellulose; in addition, the structure of the lignocellulose is significantly changed as well, and the surface of the raw material becomes rough, loose and porous, so as to greatly increase an accessible surface during enzymolysis saccharifying. The combined pretreatment process disclosed by the invention has the advantages of being not high in requirement on equipment, simple in operation, mild in processing condition, low in cost and the like, and the saccharifying efficiency of the lignocellulose is greatly improved.

The invention provides a double-body circulatory cellulose enzymatic hydrolysis reactor and an application of the double-body circulatory cellulose enzymatic hydrolysis reactor. The double-body circulatory cellulose enzymatic hydrolysis reactor specifically comprises a first reaction tank, a second reaction tank, a first pipe and a second pipe. The first reaction tank comprises a first reaction tank body, a shearing homogenizer, an inlet and an outlet, wherein the shearing homogenizer is arranged in the first reaction tank body, the inlet is used for feeding, and the outlet is used for discharging; the second reaction tank comprises more than two reaction pipes which are connected in series or parallel; the first pipe is used for connecting the first reaction tank with the second reaction tank; and the second pipe is used for connecting the reaction pipes in the second reaction tank. When being used for the enzymolysis and the saccharification of the cellulose such as the hemp bastuse, the double-body circulatory cellulos enzymatic hydrolysis reactor has the advantages of high conversion rate, high concentration, short conversion time and low enzyme preparation consumption, etc.

The invention discloses a method for improving the pretreatment effect of acid catalysis ionic liquid with an organic solvent-aqueous solution. The method comprises the following steps: (1) smashing a fiber raw material; (2) adding the raw material into an ionic liquid-acid system for pretreatment and catalysis; (3) regenerating with an organic solvent-aqueous solution in an amount which is 2 to 5 times the volume of the raw material; (4) continually stirring; (5) centrifuging to obtain a coarse regenerated fiber raw material; (6) washing the coarse regenerated fiber raw material with a corresponding organic solvent-aqueous solution by 4 to 6 times, and drying to obtain a regenerated fiber raw material. By adopting the method disclosed by the invention, the structural characteristic of the fiber raw material is changed. The dense cell wall structure of fibers is broken, the prepared regenerated fiber raw material is porous on the surface, is less in sediment, and is lower in lignin content, and the problem of adhesion of a large amount of lignin sediment to the surface of a conventional regenerated fiber raw material and the problem of inhibition of enzymatic saccharification are solved.

The invention discloses a method for strengthening the ammonia pretreatment of waste biomass by means of lignin-degrading bacteria. Particularly, on the basis of the ammonia pretreatment of the waste biomass, the lignin-degrading bacteria, namely Cupriavidus basilensis B-8, with the preservation number of CGMCC No.4240 are used, residual lignin in the waste biomass is further removed by improving culturing conditions, and the cellulose accessible surface is improved in the enzymolysis and saccharification processes. By using the method, the enzymolysis efficiency of the pretreatment of a single ammonia method is improved by about 30%, and the method has the advantages of being short in treatment time, simple in operation, small in secondary pollution, low in cost and the like.

The invention provides a high-capacity double-systematic-circulation cellulose enzymolysis reactor and its application. The high-capacity double-systematic-circulation cellulose enzymolysis reactor concretely comprises: a first reaction tank (a) comprising a tank body, shearing homogenizers positioned in the tank body, an inlet used for adding a feed material, and an outlet used for discharging the material; a second reaction tank (b) comprising above two series or parallel reaction tubes; a pipeline (c) connecting the first reaction tank with the second reaction tank; and a pipeline (d) connecting all tubes in the second reaction tank, wherein the number of the shearing homogenizers is two or more; and the volume of the first reaction tank is 3-150t, and preferably 30-100t. The enzymolysis reactor has the advantages of high conversion rate, high concentration, short conversion time, low enzyme preparation consumption, low energy consumption and the like when the enzymolysis saccharification of celluloses comprising fibrilia and the like is carried out by the enzymolysis reactor, so the enzymolysis reactor is especially suitable for high-capacity cellulose enzymolysis reaction systems.

The invention relates to a dry-method ozone pretreatment pretreater capable of increasing the straw enzymolysis saccharification ratio. The dry-method ozone pretreatment pretreater comprises a vertically arranged pretreater body, a feed hopper located on the top of the pretreater body and a discharge port formed in the bottom of the pretreater body; the feed hopper is located in the center of the pretrater top, and a diffuser is arranged under the portion beside the feed hopper in the direction perpendicular to the pretreater body; the diffuser is shaped like a circular conical surface, the vertex of the circular conical surface vertically upwards points to the center of the feed hopper, and rod-like protrusions which are perpendicular to the circular conical surface bus direction are distributed on the circular conical surface in a staggered mode; a plurality of sieve plates perpendicular to the direction of the pretreater body are arranged in the middle of the pretreater body; the sieve plates are each shaped like a mesh, and the rod-like protrusions with different heights are evenly distributed on the sieve plates and are perpendicular to the surfaces of the sieve plates. The dry-method ozone pretreatment pretreater facilitates pretreatment of semi-continuous, automatic and intelligent operation; lignin of straw subjected to ozone treatment is removed, the biodegradation rate is obviously increased, sundry bacteria and pests are killed, the storage property and fermenting property of the product are improved.

The invention provides a method for ultra-fast pretreatment of lignocellulose in biomass. The method comprises the following steps: S1, adding waste biomass into a ternary low-eutectic solvent, and sequentially carrying out microwave treatment and solid-liquid separation treatment to obtain to-be-treated filter residues, wherein the ternary low-eutectic solvent comprises a solvent prepared from choline chloride, oxalic acid and lactic acid in a molar ratio of 1:0.5:(0.5-1.5); the solid-to-liquid ratio of the waste biomass to the ternary low-eutectic solvent is 1g:(25-45)mL; and S2, cleaning the to-be-treated filter residues until the pH value is neutral, and then carrying out freeze-drying treatment to obtain the waste biomass subjected to lignocellulose pretreatment. By means of the pretreatment method, the enzymolysis efficiency can be improved; the pretreatment speed can be increased; and the treatment cost is reduced.

The invention relates to a method for improving the specific activity of cellulase and application of the method. In particular, cellulase with improved specific activity is obtained after cellulase is incubated with phenolic acid, and the cellulase with improved specific activity can be used for improving the biomass enzymolysis saccharification efficiency. Efficient biorefinery of lignocellulosic biomass is limited due to the negative impact of expensive cellulase and lignin degradation products on cellulase hydrolysis. A lignin-derived phenolic acid compound, vanillic acid, syringic acid, ferulic acid, isovanillic acid and the like can effectively improve the specific activity of cellulase, the saccharification efficiency of different biomass is effectively improved under the condition that the enzyme dosage is not increased, and the enzyme use cost of biomass enzymolysis saccharification can be effectively reduced.