34results about How to "Improve enzymatic hydrolysis and saccharification efficiency" patented technology

The invention discloses a method for reinforcing the waste biomass sodium carbonate pretreatment by utilizing lignin-degrading bacteria. According to the method, based on waste biomass sodium carbonate pretreatment, residual lignin and hemicellulose in waste biomass are further removed by utilizing the lignin-degrading bacterium Cupriavidus basilensis B-8 having a preservation number of CGMCC No.4240 and improving the culture condition, steady straight rod state of waste biomass is loosened, and the surface of the waste biomass shows a porous flake shape, so that the accessible surface during enzymolysis saccharification is greatly improved. The method can be used for improving the enzymolysis rate of sodium carbonate sodium pretreatment by nearly 40 percent with remarkable effect, and has the advantages of being short in treatment time, simple in operation, small in secondary pollution, low in cost and the like.

The invention discloses a method for enhancing waste biomass hydro-thermal pretreatment by using lignin degrading bacteria, and concreatly relates to a method that based on pretreatment of waste biomass by a hydrothermal method, the lignin degrading bacteria (Cupriavidus basilensis B-8, a preservation number is CGMCC No.4240) is used, the culture condition is improved, and the residual lignin in the waste biomass is removed, and the accessible surfaces while enzymatic hydrolysis and saccharification can be greatly increased. The enzymatic hydrolysis efficiency for hydrothermal method pretreatment is increased by about 56%, and the method has the advantages of short processing time, simple operation, no secondary pollution, and low cost.

The invention discloses a method for improving the pretreatment effect of acid catalysis ionic liquid with an organic solvent-aqueous solution. The method comprises the following steps: (1) smashing a fiber raw material; (2) adding the raw material into an ionic liquid-acid system for pretreatment and catalysis; (3) regenerating with an organic solvent-aqueous solution in an amount which is 2 to 5 times the volume of the raw material; (4) continually stirring; (5) centrifuging to obtain a coarse regenerated fiber raw material; (6) washing the coarse regenerated fiber raw material with a corresponding organic solvent-aqueous solution by 4 to 6 times, and drying to obtain a regenerated fiber raw material. By adopting the method disclosed by the invention, the structural characteristic of the fiber raw material is changed. The dense cell wall structure of fibers is broken, the prepared regenerated fiber raw material is porous on the surface, is less in sediment, and is lower in lignin content, and the problem of adhesion of a large amount of lignin sediment to the surface of a conventional regenerated fiber raw material and the problem of inhibition of enzymatic saccharification are solved.

The invention discloses a method for strengthening Fenton reaction pre-treatment of waste biomasses by utilizing a lignin-degrading bacterium. Specifically, on the basis of the Fenton reaction pre-treatment of the waste biomasses, residual lignin in the waste biomasses is further removed by utilizing the lignin-degrading bacterium (Cupriavidus basilensis B-8 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 4240) and improving culture conditions; a stable straight rod shape of the waste biomasses is changed into a loose laminar shape, so that an accessible surface in an enzymolysis and saccharification process is improved. By adopting the method disclosed by the invention, the enzymolysis efficiency of the Fenton reaction pre-treatment can be improved by about 60 percent and can be improved by about 120 percent at maximum; the method has the advantages of short treatment time, simple operation, small secondary pollution, low cost and the like.

The invention discloses a biological-chemical combined treatment process for improving saccharifying effect of lignocellulose. According to the process, a lignocellulose raw material is subjected to biological treatment by virtue of an efficient lignocelluloses degrading bacterium sphingobacterium sp. LD-1 (preservation number is CGMCC No.10920), so as to remove partial lignin and hemicellulose components in the lignocellulose raw material with the action of microorganisms, and to damage the stable structure of the lignocellulose to a certain degree; and furthermore, the lignocellulose undergoes chemical treatment at low temperature by virtue of alkali / urea, so that the lignocellulose is more loose in structure. The lignocellulose, which is preprocessed by the process disclosed by the invention, achieves significant change in component content and obvious increase in proportion of cellulose; in addition, the structure of the lignocellulose is significantly changed as well, and the surface of the raw material becomes rough, loose and porous, so as to greatly increase an accessible surface during enzymolysis saccharifying. The combined pretreatment process disclosed by the invention has the advantages of being not high in requirement on equipment, simple in operation, mild in processing condition, low in cost and the like, and the saccharifying efficiency of the lignocellulose is greatly improved.

The invention provides a high-capacity double-systematic-circulation cellulose enzymolysis reactor and its application. The high-capacity double-systematic-circulation cellulose enzymolysis reactor concretely comprises: a first reaction tank (a) comprising a tank body, shearing homogenizers positioned in the tank body, an inlet used for adding a feed material, and an outlet used for discharging the material; a second reaction tank (b) comprising above two series or parallel reaction tubes; a pipeline (c) connecting the first reaction tank with the second reaction tank; and a pipeline (d) connecting all tubes in the second reaction tank, wherein the number of the shearing homogenizers is two or more; and the volume of the first reaction tank is 3-150t, and preferably 30-100t. The enzymolysis reactor has the advantages of high conversion rate, high concentration, short conversion time, low enzyme preparation consumption, low energy consumption and the like when the enzymolysis saccharification of celluloses comprising fibrilia and the like is carried out by the enzymolysis reactor, so the enzymolysis reactor is especially suitable for high-capacity cellulose enzymolysis reaction systems.

The invention relates to a production process of waxy corn rice wine, which specifically includes the following steps: 1), cleaning waxy corn seeds, beating, and sieving; 2), adding a compound enzyme preparation for enzymatic hydrolysis and saccharification; 3), adding activated corn Yellow rice wine yeast and yellow rice wine koji, add lactic acid to adjust the pH value and then carry out primary fermentation; 4) Add compound aroma-enhancing yeast for secondary fermentation; 5) Filter through diatomite, then filter through a ceramic microporous membrane filter, and finally pass through a tube Type sterilizer high temperature instant sterilization passivation, after sealing enters the aging stage. The invention solves the problem of high content of urethane while improving the production efficiency, ensures rich nutritional value and good taste of rice wine products, and is suitable for large-scale automatic production.

The present invention provides a dual body circulatory reactor for cellulose enzymolysis and an application thereof. Specifically, the present invention provides a reactor for cellulose enzymolysis comprising: (a) a first reaction tank comprising a tank body, a shear homogenizer provided in the tank body, an inlet for feeding, and an outlet for discharging a material; (b) a second reaction tank comprising two or more reaction tubes connected in series or in parallel; (c) a pipeline connecting the first and the second reaction tanks; and (d) a pipeline connecting each tube bodies in the second reaction tank. When conducting enzyme saccharification on bast fiber crops and other celluloses, the enzymolysis reactor of the present invention has advantages of high conversion rate, high concentration, short conversion time, low enzyme consumption, and low energy consumption, and thus is especially suitable for a large volume cellulose enzymolysis reaction system.

The invention relates to a dry-method ozone pretreatment pretreater capable of increasing the straw enzymolysis saccharification ratio. The dry-method ozone pretreatment pretreater comprises a vertically arranged pretreater body, a feed hopper located on the top of the pretreater body and a discharge port formed in the bottom of the pretreater body; the feed hopper is located in the center of the pretrater top, and a diffuser is arranged under the portion beside the feed hopper in the direction perpendicular to the pretreater body; the diffuser is shaped like a circular conical surface, the vertex of the circular conical surface vertically upwards points to the center of the feed hopper, and rod-like protrusions which are perpendicular to the circular conical surface bus direction are distributed on the circular conical surface in a staggered mode; a plurality of sieve plates perpendicular to the direction of the pretreater body are arranged in the middle of the pretreater body; the sieve plates are each shaped like a mesh, and the rod-like protrusions with different heights are evenly distributed on the sieve plates and are perpendicular to the surfaces of the sieve plates. The dry-method ozone pretreatment pretreater facilitates pretreatment of semi-continuous, automatic and intelligent operation; lignin of straw subjected to ozone treatment is removed, the biodegradation rate is obviously increased, sundry bacteria and pests are killed, the storage property and fermenting property of the product are improved.

The invention discloses a method for strengthening the ammonia pretreatment of waste biomass by means of lignin-degrading bacteria. Particularly, on the basis of the ammonia pretreatment of the waste biomass, the lignin-degrading bacteria, namely Cupriavidus basilensis B-8, with the preservation number of CGMCC No.4240 are used, residual lignin in the waste biomass is further removed by improving culturing conditions, and the cellulose accessible surface is improved in the enzymolysis and saccharification processes. By using the method, the enzymolysis efficiency of the pretreatment of a single ammonia method is improved by about 30%, and the method has the advantages of being short in treatment time, simple in operation, small in secondary pollution, low in cost and the like.