Waste biomass recycling method

A technology for waste biomass and recycling, which is applied in the field of waste biomass recycling technology, can solve problems such as waste of lignin resources, and achieve the effects of large dosage of chemicals, improved efficiency of enzymatic hydrolysis and saccharification, and reduced strength.

Active Publication Date: 2017-10-24
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problem of waste of lignin resources in the existing waste biomass and improve the utilization rate of waste biomass, the present invention provides a method for utilizing lignin-degrading bacteria to realize waste biomass resource utilization

Method used

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Examples

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Embodiment 1

[0039] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0040] (2) Put the waste biomass in a container of appropriate size, add NaOH solution with a concentration of 2% according to the solid-liquid ratio of 1:10 (g / ml), let it stand in a constant temperature environment of 121°C for 60 minutes, and then filter and separate to obtain Wet slag A1 and treated black liquor B.

[0041] (3) Rinse the wet residue A1 obtained by filtering and separating with distilled water repeatedly until the pH of the washing solution is neutral, and dry at 60° C. to a constant weight to obtain dry residue A2.

[0042] (4) Inoculate the Cupriavidus basilensis B-8 cells stored on the LB slope into LB liquid medium, and culture at 30°C for 18 hours (the optical density at 600nm reaches 0.8-1.0) to obtain the seeds of Cupriavidus basilensis B-8 Liquid; wherein said LB liquid culture medium each composition ratio ...

Embodiment 2

[0052] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0053] (2) Put waste biomass in a container of appropriate size, add NaOH solution with a concentration of 2% according to the solid-liquid ratio of 1:10 (g / ml), let it stand in a constant temperature environment of 121°C for 15 minutes, and then filter and separate to obtain Wet slag A1 and treated black liquor B.

[0054] (3) Rinse the wet residue A1 obtained by filtering and separating with distilled water repeatedly until the pH of the washing solution is neutral, and dry at 60° C. to a constant weight to obtain dry residue A2.

[0055] (4) Inoculate the Cupriavidus basilensis B-8 bacteria stored on the LB slant into LB liquid medium, and cultivate at 30°C for 18 hours (that is, the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensis B-8 Seed liquid; wherein the distribution ratio of the components of the LB ...

Embodiment 3

[0065] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0066] (2) Put waste biomass in a container of appropriate size, add NaOH solution with a concentration of 1% according to a solid-to-liquid ratio of 1:10 (g / ml), let it stand in a constant temperature environment of 121°C for 30 minutes, and then filter and separate to obtain Wet slag A1 and treated black liquor B.

[0067] (3) Rinse the wet residue A1 obtained by filtering and separating with distilled water repeatedly until the pH of the washing solution is neutral, and dry at 60° C. to a constant weight to obtain dry residue A2.

[0068] (4) Inoculate the Cupriavidus basilensis B-8 bacteria stored on the LB slant into LB liquid medium, and cultivate at 30°C for 18 hours (that is, the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensis B-8 Seed liquid; wherein the distribution ratio of the components of the LB l...

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Abstract

The invention discloses a waste biomass recycling method which comprises the following specific steps: (1) performing pre-treatment by means of a waste biomass alkali method to obtain black liquor and alkali pre-treated residues; (2) after actiononthe black liquor by Cupriavidus basilensis B-8, the preservation number of which is GCMCC No.4240, separating and purifying a polyhydroxyalkanoate biological plastic in the thalli thereof; (3) treating the alkali pre-treated residues by the Cupriavidus basilensis B-8 so as to deeply remove residual lignin and converting the same into the biological plastic; and (4) performing enzymolysis on alkali-biological treatment residues to obtain high purity sugar as a follow-up biological fermenting raw material. According to the method disclosed by the invention, the sugar yielding amount by enzymolysis of waste biomass is improved by about 10 times, and lignin in residues and black liquor can be converted into the biological plastic at the same time, so that the environmental pollution problem caused by the pre-treated black liquor is solved, and the economical benefit of the process is also remarkably improved, and the method has the advantages of short treatment time, simplicity in operation, small secondary pollution, low cost and the like.

Description

Technical field: [0001] The invention belongs to the technical field of biomass new energy, and in particular relates to a process for utilizing microorganisms to realize recycling of waste biomass. Background technique: [0002] With the depletion of fossil energy worldwide, the development and utilization of renewable energy has become a top priority. Compared with the first-generation biomass represented by glucose and starch, the second-generation biomass represented by agricultural and forestry waste avoids the problems of rising costs, competing with people for food and land, and becomes the most suitable alternative One of energy. As an effective component of agricultural and forestry waste, lignocellulose is the most abundant and cheapest renewable resource on earth. It is mainly composed of cellulose, hemicellulose and lignin. Among them, cellulose is a long-chain molecule formed by D-glucose through β-1,4 glycosidic bonds, and the long-chain molecule further for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62B09B3/00C12R1/01
CPCB09B3/00C12P7/625C12N1/205C12R2001/01
Inventor 柴立元石岩颜旭司梦莹刘明人杨志辉
Owner CENT SOUTH UNIV
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