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A high-efficiency green pretreatment method and application for improving lignocellulose enzymatic hydrolysis and saccharification efficiency

A lignocellulosic and pretreatment technology, applied in the direction of biofuel, fermentation, etc., to achieve the effect of improving the efficiency of enzymatic hydrolysis and saccharification, low cost and simple operation

Active Publication Date: 2020-12-11
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems existing in the existing lignocellulose pretreatment technology, the present invention provides a NADES system consisting of choline chloride / citric acid / glycerol, which is applied to the lignocellulose pretreatment, greatly improving the enzyme Glycation efficiency

Method used

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  • A high-efficiency green pretreatment method and application for improving lignocellulose enzymatic hydrolysis and saccharification efficiency
  • A high-efficiency green pretreatment method and application for improving lignocellulose enzymatic hydrolysis and saccharification efficiency
  • A high-efficiency green pretreatment method and application for improving lignocellulose enzymatic hydrolysis and saccharification efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] ( ) The rice straw is dried and crushed, and the particle size is controlled to be 180-425 μm;

[0021] ( ) Mix citric acid, choline chloride, and glycerol at a molar ratio of 0.5:1:1.5, put them in a shaker at 100 ℃, and wait until a completely fused colorless and transparent liquid is formed, take it out for use;

[0022] ( ) into rice straw at a content of 10% (W / W), adding the above ( ) in eutectic solvent, add 5% (W / W) distilled water and vibrate and mix in a shaker at 130 ℃ for 4 hours after pretreatment, filter and wash the filter residue with distilled water until the pH of the cleaning solution is neutral. The filtered residue was dried at 55°C to obtain NADES-treated rice straw samples;

[0023] ( ) The rice straw sample pretreated by NADES was mixed with citrate buffer (0.1 mol / L, pH 4.8) at a concentration of 25 mg / mL, and then 12 FPU / g substrate cellulase was added, at 50 After enzymatic hydrolysis for 72 h under the conditions of ℃ and 100 rpm / m...

Embodiment 2

[0026] ( ) The rice straw is dried and crushed, and the particle size is controlled to be 180-425 μm;

[0027] ( ) Mix citric acid, choline chloride, and glycerol at a molar ratio of 1:1:1, place in a shaker at 100 °C, and wait until a completely fused colorless and transparent liquid is formed, take it out for use;

[0028] ( ) into rice straw at a content of 10% (W / W), adding the above ( ) in eutectic solvent, add 5% (W / W) distilled water and vibrate and mix in a shaker at 130 ℃ for 4 hours after pretreatment, filter and wash the filter residue with distilled water until the pH of the cleaning solution is neutral. The filtered residue was dried at 55°C to obtain NADES-treated rice straw samples;

[0029] ( ) The rice straw sample pretreated by NADES was mixed with citrate buffer (0.1 mol / L, pH 4.8) at a concentration of 25 mg / mL, and then 12 FPU / g substrate cellulase was added, at 50 After enzymatic hydrolysis for 72 h under the conditions of ℃ and 100 rpm / min, the ...

Embodiment 3

[0032] (a) Rice straw is dried and crushed, and the particle size is controlled to be 180-425 μm;

[0033] ( ) Mix citric acid, choline chloride, and glycerol at a molar ratio of 0.5:1:1.5, put them in a shaker at 100 ℃, and wait until a completely fused colorless and transparent liquid is formed, take it out for use;

[0034] (c) Add rice straw at a content of 10% (W / W), add the above ( ) in eutectic solvent, add 5% (W / W) distilled water and vibrate and mix in a shaker at 130 ℃ for 3 hours after pretreatment, filter and wash the filter residue with distilled water until the pH of the cleaning solution is neutral. The filtered residue was dried at 55°C to obtain NADES-treated rice straw samples;

[0035] ( ) The rice straw sample pretreated by NADES was mixed with citrate buffer (0.1 mol / L, pH 4.8) at a concentration of 25 mg / mL, and then 12 FPU / g substrate cellulase was added, at 50 After enzymatic hydrolysis for 72 h under the conditions of ℃ and 100 rpm / min, the enzy...

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Abstract

The invention utilizes NADES (Natural Deep Eutectic Solvent) preprocessing to improve the lignocellulose enzymolysis and saccharification efficiency. Choline chloride, citric acid and glycerol are mixed at a certain ratio, a certain quantity of distilled water is added to process lignocellulose raw materials, parts of lignin and hemicellulose are removed, and the compact structure of the lignocellulose is damaged to a certain degree. According to the processing method, the ratio of lignin and hemicellulose components in the lignocellulose can be reduced, the surface of the lignocellulose is severely damaged, and the saccharification efficiency of the lignocellulose is improved. The preprocessing method has the advantages of low cost, environment protection, low equipment requirement, simpleness in operation and the like, and the lignocellulose enzymolysis and saccharification efficiency of the lignocellulose can be greatly improved.

Description

technical field [0001] The invention relates to the technical field of pretreatment of bioethanol from lignocellulose, specifically a natural deep eutectic solvent (NADES) choline chloride / citric acid / glycerol pretreatment to improve the enzymatic hydrolysis and saccharification efficiency of lignocellulose Methods. Background technique [0002] Under the background of the world's strong demand for energy and the depletion of traditional fossil energy, renewable bioenergy has attracted more and more attention. Lignocellulose is the largest renewable resource on earth, and using it as a raw material to produce fuel ethanol is considered to be one of the effective ways to solve the energy crisis. The conversion of lignocellulose into fuel ethanol mainly includes three processes: pretreatment, enzymatic saccharification, and fermentation. Pretreatment is to remove or partially remove lignin, improve the porosity of the raw material matrix, destroy the structure of lignin, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14
CPCC12P7/10C12P19/14C12P2201/00Y02E50/10
Inventor 陈跃辉张斌斌
Owner XIANGTAN UNIV
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