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A method of using lignin-degrading bacteria to strengthen the acid pretreatment of waste biomass

A technology of lignin degrading bacteria and waste biomass, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of mild reaction conditions, simple operation, and small secondary pollution

Active Publication Date: 2020-11-17
CENT SOUTH UNIV
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Problems solved by technology

[0005] In order to solve the problems existing in the existing waste biomass pretreatment technology, the present invention provides a method of using lignin-degrading bacteria to strengthen the waste biomass acid pretreatment method

Method used

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  • A method of using lignin-degrading bacteria to strengthen the acid pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the acid pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the acid pretreatment of waste biomass

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Embodiment 1

[0028] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0029] (2) Put the rice straw in a container of appropriate size, add sulfuric acid solutions with a concentration of 0.5%, 1.0%, 1.5% and 2.0% respectively according to the solid-liquid ratio of 1:10 (g / ml), and place them separately After 20, 30 and 40 minutes in a constant temperature environment at 121°C, wet residue A was obtained by filtration and separation.

[0030] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0031] (4) Inoculate the B-8 bacteria stored on the LB slope into the LB liquid medium, and cultivate at 30° C. for 18 hours (that is, the optical density at 600 nm reaches 0.8-1.0) to obtain the seed liquid of B-8; Wherein the proportion of each component of ...

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Abstract

The invention discloses a method for enhancing waste biomass acid process pretreatment by a lignin-degrading bacterium. Particularly, on the basis of the waste biomass acid process pretreatment, the lignin-degrading bacteria (Cupriavidus basilensis B-8 with the preservation code of CGMC No.4240) is used; the residue lignin in the waste biomass can be further removed through improving the culturing condition; the surface compact stable and firm structure of the waste biomass can be thoroughly broken; the reached surface during the zymohydrolysis saccharification is improved. The method has the advantages that the zymohydrolysis efficiency of the acid process pretreatment is improved to 62 percent to the maximum degree; the effect is obvious; the advantages of mild reaction conditions, short treatment time, simple operation, small secondary pollution, low cost and the like are realized.

Description

Technical field: [0001] The invention belongs to the technical field of new biomass energy, and in particular relates to a method for using lignin-degrading bacteria to strengthen the acid pretreatment of waste biomass. Background technique: [0002] In recent years, in order to reduce the dependence on fossil fuels, governments and scientific research institutions of various countries have vigorously carried out the development and research of renewable energy. Lignocellulose in waste biomass is mainly composed of cellulose, hemicellulose and lignin, which contains a huge amount of carbon resources. It has become the second generation of biomass energy and is currently a green product such as bioethanol, bioplastics, and biodiesel. The most important carbon source for biological fermentation. The hemicellulose and lignin in lignocellulose are tightly embedded around the cellulose, and without any treatment, the efficiency of saccharification and fermentation is extremely l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12R1/01
CPCC12P19/14C12P2201/00
Inventor 颜旭柴立元石岩王钟任黎佳未杨志辉
Owner CENT SOUTH UNIV
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