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A method of using lignin-degrading bacteria to strengthen the ammonia pretreatment of waste biomass

A technology for lignin degrading bacteria and waste biomass, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of improving the efficiency of enzymatic hydrolysis and saccharification, with less secondary pollution and short treatment time.

Active Publication Date: 2020-09-01
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems existing in the existing waste biomass pretreatment technology, the present invention provides a method of using lignin-degrading bacteria to strengthen the ammonia pretreatment of waste biomass

Method used

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  • A method of using lignin-degrading bacteria to strengthen the ammonia pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the ammonia pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the ammonia pretreatment of waste biomass

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Experimental program
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Effect test

Embodiment 1

[0028] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0029] (2) Put lignocellulose in a container of appropriate size, add ammonia solution with a concentration of 0.5% according to the solid-to-liquid ratio of 1:10 (g / ml), let it stand in a constant temperature environment of 160°C for 30 minutes, and then filter and separate to obtain Wet residue A.

[0030] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0031] (4) Inoculate the Cupriavidus basilensis B-8 bacteria stored on the LB solid plate in the LB liquid medium, and culture at 30°C for 18 hours (the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensis B-8 The seed liquid of the LB solid culture medium wherein said LB solid medium each composition ra...

Embodiment 2

[0038](1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0039] (2) Further place lignocellulose in a container of appropriate size, add ammonia solution with a concentration of 0.5% according to the solid-liquid ratio of 1:10 (g / ml), and stand in a constant temperature environment of 180°C for 30 minutes, then filter and separate Wet slag A was obtained.

[0040] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0041] (4) Inoculate the Cupriavidus basilensisB-8 thalline preserved on the LB solid plate in the LB liquid medium, and cultivate it for 18h at a temperature of 30°C (the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensisB-8 Seed liquid; wherein the proportion of each component of the LB solid medium i...

Embodiment 3

[0048] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0049] (2) Put lignocellulose in a container of appropriate size, add ammonia solution with a concentration of 0.5% according to the solid-to-liquid ratio of 1:10 (g / ml), and place it in a constant temperature environment of 200°C for 30 minutes, then filter and separate to obtain Wet residue A.

[0050] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0051] (4) Inoculate the Cupriavidus basilensisB-8 thalline preserved on the LB solid plate in the LB liquid medium, and cultivate it for 18h at a temperature of 30°C (the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensisB-8 Seed liquid; wherein the proportion of each component of the LB solid medium is: ...

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Abstract

The invention discloses a method for strengthening the ammonia pretreatment of waste biomass by means of lignin-degrading bacteria. Particularly, on the basis of the ammonia pretreatment of the waste biomass, the lignin-degrading bacteria, namely Cupriavidus basilensis B-8, with the preservation number of CGMCC No.4240 are used, residual lignin in the waste biomass is further removed by improving culturing conditions, and the cellulose accessible surface is improved in the enzymolysis and saccharification processes. By using the method, the enzymolysis efficiency of the pretreatment of a single ammonia method is improved by about 30%, and the method has the advantages of being short in treatment time, simple in operation, small in secondary pollution, low in cost and the like.

Description

Technical field: [0001] The invention belongs to the technical field of new biomass energy, and in particular relates to a method for using lignin-degrading bacteria to strengthen ammonia pretreatment of waste biomass. Background technique: [0002] Today, many countries are formulating or adjusting their own energy policies, placing biomass energy in an important position. According to the characteristics of energy distribution in my country, the second-generation biomass, which uses agricultural and forestry waste as raw materials, has been paid attention to and has become one of the current alternative energy sources. Agricultural and forestry wastes are natural lignocellulosic resources, mainly composed of cellulose, hemicellulose and lignin, of which the lignin content is about 5% to 30%. Lignin is a complex, amorphous polymer composed of phenylpropane units connected by ether bonds and carbon-carbon bonds. It is because of the natural stable structure of lignin that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12R1/01
CPCC12P19/14C12P2201/00
Inventor 颜旭柴立元石岩卓胜男刘丹杨志辉
Owner CENT SOUTH UNIV
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