A method of using lignin-degrading bacteria to strengthen the ammonia pretreatment of waste biomass
A technology for lignin degrading bacteria and waste biomass, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of improving the efficiency of enzymatic hydrolysis and saccharification, with less secondary pollution and short treatment time.
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Embodiment 1
[0028] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.
[0029] (2) Put lignocellulose in a container of appropriate size, add ammonia solution with a concentration of 0.5% according to the solid-to-liquid ratio of 1:10 (g / ml), let it stand in a constant temperature environment of 160°C for 30 minutes, and then filter and separate to obtain Wet residue A.
[0030] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.
[0031] (4) Inoculate the Cupriavidus basilensis B-8 bacteria stored on the LB solid plate in the LB liquid medium, and culture at 30°C for 18 hours (the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensis B-8 The seed liquid of the LB solid culture medium wherein said LB solid medium each composition ra...
Embodiment 2
[0038](1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.
[0039] (2) Further place lignocellulose in a container of appropriate size, add ammonia solution with a concentration of 0.5% according to the solid-liquid ratio of 1:10 (g / ml), and stand in a constant temperature environment of 180°C for 30 minutes, then filter and separate Wet slag A was obtained.
[0040] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.
[0041] (4) Inoculate the Cupriavidus basilensisB-8 thalline preserved on the LB solid plate in the LB liquid medium, and cultivate it for 18h at a temperature of 30°C (the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensisB-8 Seed liquid; wherein the proportion of each component of the LB solid medium i...
Embodiment 3
[0048] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.
[0049] (2) Put lignocellulose in a container of appropriate size, add ammonia solution with a concentration of 0.5% according to the solid-to-liquid ratio of 1:10 (g / ml), and place it in a constant temperature environment of 200°C for 30 minutes, then filter and separate to obtain Wet residue A.
[0050] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.
[0051] (4) Inoculate the Cupriavidus basilensisB-8 thalline preserved on the LB solid plate in the LB liquid medium, and cultivate it for 18h at a temperature of 30°C (the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensisB-8 Seed liquid; wherein the proportion of each component of the LB solid medium is: ...
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