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A method of using lignin-degrading bacteria to strengthen the fenton reaction pretreatment of waste biomass

A technology of lignin-degrading bacteria and waste biomass, applied in the direction of microorganism-based methods, using microorganism/enzyme cellulose treatment, biochemical equipment and methods, etc., to achieve the effects of small secondary pollution, short processing time and simple operation

Active Publication Date: 2020-11-17
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems existing in the existing waste biomass Fenton reaction pretreatment technology, the present invention provides a method of using lignin-degrading bacteria to strengthen the waste biomass Fenton reaction pretreatment method

Method used

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  • A method of using lignin-degrading bacteria to strengthen the fenton reaction pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the fenton reaction pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the fenton reaction pretreatment of waste biomass

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Experimental program
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Effect test

Embodiment 1

[0029] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0030] (2) Further put the waste biomass in a container of appropriate size, and add FeCl with a concentration of 0.02mol / L at a solid-to-liquid ratio of 1:10 (g / ml) 3 and 1.5mol / L of H 2 o 2 , shaken at room temperature for 2h, and separated by filtration to obtain wet residue A.

[0031] (3) The wet slag A obtained through filtration and separation was further rinsed repeatedly with distilled water until the pH of the rinsing solution was neutral, and dried at 60° C. to a constant weight to obtain dry slag B.

[0032] (4) Inoculate the B-8 bacteria stored on the LB slope into the LB liquid medium, and cultivate at 30° C. for 18 hours (the optical density at 600 nm reaches 0.8-1.0) to obtain the seed liquid of B-8; The distribution ratio of the components of the LB liquid medium is: 10 g of peptone, 5 g of yeast powder, 10 g of sodi...

Embodiment 2

[0039] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0040] (2) Further put the waste biomass in a container of appropriate size, and add FeCl with a concentration of 0.03mol / L at a solid-to-liquid ratio of 1:10 (g / ml) 3 and 2.25mol / L of H 2 o 2 , shaken at room temperature for 2h, and separated by filtration to obtain wet residue A.

[0041] (3) The wet slag A obtained through filtration and separation was further rinsed repeatedly with distilled water until the pH of the rinsing solution was neutral, and dried at 60° C. to a constant weight to obtain dry slag B.

[0042] (4) Inoculate the B-8 bacteria stored on the LB slope into the LB liquid medium, and cultivate at 30° C. for 18 hours (the optical density at 600 nm reaches 0.8-1.0) to obtain the seed liquid of B-8; The distribution ratio of the components of the LB liquid medium is: 10 g of peptone, 5 g of yeast powder, 10 g of sod...

Embodiment 3

[0049] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0050] (2) Further put the waste biomass in a container of appropriate size, and add FeCl with a concentration of 0.01mol / L at a solid-to-liquid ratio of 1:10 (g / ml) 3 and 0.75mol / L of H 2 o 2 , shaken at room temperature for 2h, and separated by filtration to obtain wet residue A.

[0051] (3) The wet slag A obtained through filtration and separation was further rinsed repeatedly with distilled water until the pH of the rinsing solution was neutral, and dried at 60° C. to a constant weight to obtain dry slag B.

[0052] (4) Inoculate the B-8 bacteria stored on the LB slope into the LB liquid medium, and cultivate at 30° C. for 18 hours (the optical density at 600 nm reaches 0.8-1.0) to obtain the seed liquid of B-8; The distribution ratio of the components of the LB liquid medium is: 10 g of peptone, 5 g of yeast powder, 10 g of sod...

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Abstract

The invention discloses a method for strengthening Fenton reaction pre-treatment of waste biomasses by utilizing a lignin-degrading bacterium. Specifically, on the basis of the Fenton reaction pre-treatment of the waste biomasses, residual lignin in the waste biomasses is further removed by utilizing the lignin-degrading bacterium (Cupriavidus basilensis B-8 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 4240) and improving culture conditions; a stable straight rod shape of the waste biomasses is changed into a loose laminar shape, so that an accessible surface in an enzymolysis and saccharification process is improved. By adopting the method disclosed by the invention, the enzymolysis efficiency of the Fenton reaction pre-treatment can be improved by about 60 percent and can be improved by about 120 percent at maximum; the method has the advantages of short treatment time, simple operation, small secondary pollution, low cost and the like.

Description

Technical field: [0001] The invention belongs to the technical field of biomass new energy, and in particular relates to a method for strengthening the Fenton reaction pretreatment of waste biomass by using lignin-degrading bacteria. Background technique: [0002] In recent years, in order to reduce the dependence on fossil fuels, governments and scientific research institutions of various countries have vigorously carried out the development and research of renewable energy. Lignocellulose in waste biomass is mainly composed of cellulose, hemicellulose and lignin, which contains a huge amount of carbon resources. It has become the second generation of biomass energy and is currently a green product such as bioethanol, bioplastics, and biodiesel. The most important carbon source for biological fermentation. The hemicellulose and lignin in lignocellulose are tightly embedded around the cellulose, and without any treatment, the efficiency of saccharification and fermentation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14D21C5/00C12R1/01
CPCC12P19/14C12P2201/00D21C5/005
Inventor 石岩柴立元颜旭张可菁王钟任杨志辉
Owner CENT SOUTH UNIV
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