Comprehensive utilizing method of sweet sorghum stalks

A technology of sorghum stalks and mixed liquor, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of low utilization rate of distiller's grains and low added value of products, and achieve low enzyme cost and high accessibility , high conversion rate effect

Inactive Publication Date: 2012-04-04
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a comprehensive utilization method of sweet sorgh

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1

[0037] Harvest mature sweet sorghum, using sweet sorghum stalks with a total sugar content of 11% and reducing sugar content of 7% as raw materials, crush them into filaments with a diameter of 1 to 2 mm and a length less than 30 mm, and adjust the water content of the crushed sweet sorghum stalks with deionized water Is 70%. The TSH-Sc-001 strain is added to the closed silo, mixed with the crushed materials, and then continuously fermented in the continuous solid fermenter. The amount of yeast inoculation is 10% (that is, the ratio of crushed material to yeast liquid is 1kg: 100mL, and each mL of yeast liquid contains about 1×10 7 Yeast cells), adjust the operating parameters, keep the temperature in the tank at 30-35°C, the rotation speed of the fermenter at 0.25rpm, and the fermentation time for 30h. Add 200 mL of distilled water to every 50 g of fermented material, heat and distill, and collect the condensate, and stop when the collected condensate reaches...

Example Embodiment

[0045] Example 2

[0046] The solid-state fermentation method of sweet sorghum stalks is the same as in Example 1.

[0047] Take 3g of distiller's grains (dry weight) into the microwave digestion apparatus, add 27mL of 3% oxalic acid, and acidolyze for 1.5h at 130°C. After the reaction is completed, suction filtration is performed to obtain xylose solution and acid hydrolysis residue.

[0048] The yield of xylose was 21.48%. The composition of the acid hydrolysis residue is: cellulose 52.63%, hemicellulose 11.67%, lignin 20.60%, and ash content 0.70%.

[0049] The enzymatic hydrolysis method of the acid hydrolysis residue is the same as in Example 1. The conversion rate of enzymatic hydrolysis and saccharification of cellulose for 72 hours was 85.56%, and the concentration of glucose was 24.30 g / L.

[0050] The fermentation conditions of the enzymatic hydrolysis mixture were the same as in Example 1. The ethanol yield reaches 90%.

[0051] Both the amount of xylose in the acid hydrol...

Example Embodiment

[0052] Example 3

[0053] The solid-state fermentation method of sweet sorghum stalks is the same as in Example 1.

[0054] Take 3g of distiller's grains (dry weight) into a high-temperature sterilization pot, and use 27mL of 3% oxalic acid for acid hydrolysis at 125°C for 0.5h. After the reaction is completed, suction filtration is performed to obtain xylose solution and acid hydrolysis residue.

[0055] The yield of xylose was 21.37%. The composition of the acid hydrolysis residue is: cellulose 51.03%, hemicellulose 6.99%, lignin 19.25%, and ash content 0.73%.

[0056] The enzymatic hydrolysis method of the acid hydrolysis residue is the same as in Example 1. The conversion rate of enzymatic hydrolysis and saccharification 48h of cellulose was 65.22%, and the concentration of glucose was 18.19g / L.

[0057] The fermentation conditions of the enzymatic hydrolysis mixture were the same as in Example 1. The ethanol yield reaches 90%.

[0058] Both the amount of xylose in the acid hydro...

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Abstract

The invention discloses a comprehensive utilizing method of sweet sorghum stalks belonging to the technical field of biomass energy and chemical industry. The method comprises the following steps of: 1) fermenting sweet sorghum stalks in a solid state and distilling the sweet sorghum stalks to obtain an ethanol solution and distillers' grains; 2) hydrolyzing the distillers' grains under an acidic condition; and carrying out solid and liquid separation to obtain an xylose solution and an acidolysis slag; 3) washing the acidolysis slag by using water as an enzymatic substrate; adding an enzymolysis buffer solution; adding enzyme to carrying out enzymolysis; and then carrying out solid and liquid separation to obtain an enzymolysis mixed solution and enzymolysis xylogen; and 4) inoculating the enzymolysis mixed solution into yeast and fermenting the enzymolysis mixed solution to generate an ethanol solution. According to the invention, a new way is found for utilizing the distillers' grains; and xylose with high value added is obtained; meanwhile, the cellulose content and cellulose accessibility in the acidolysis slag are increased so that high conversion rate of cellulose enzymolysis and saccharification is realized. By using the method disclosed by the invention, the utilization rate of the lignocellulose raw material with high sugar content is increased, the ethanol yield per unit crop is increased, and the production cost of the cellulose ethanol is reduced.

Description

Technical field [0001] The invention belongs to the technical field of biomass energy and chemical industry, and specifically relates to a comprehensive utilization method of sweet sorghum stalks after solid fermentation to produce ethanol. technical background [0002] As an energy crop, sweet sorghum has remarkable advantages. Sweet sorghum has a high photosynthesis rate and extremely high biological yield. The stalks of sweet sorghum are rich in sugar. The stalks of sweet sorghum can ferment to produce 3000-5000L of ethanol per hectare, depending on the variety. [0003] The water requirement for planting sweet sorghum is only 1 / 3 to 1 / 5 of that of sugarcane, and it has a wide range of adaptability to soil conditions, especially its remarkable drought resistance and salt and alkali resistance. Sweet sorghum can be grown on marginal land such as wasteland, arid land and saline-alkali land, and it can be grown three times a year. Planting sweet sorghum to produce fuel ethanol h...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P19/14C12P7/22C13K13/00C12R1/865
CPCY02E50/16Y02E50/10
Inventor 李十中李纪红闫智培
Owner TSINGHUA UNIV
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