160 results about "Sugar yield" patented technology

In a process for converting lingnocellulosic biomass to ethanol, the improvement of obtaining higher fermentable soluble sugar yields by drying acid impregnated biomass particles, comprising: a) feeding moist lignocellulosic biomass into an acid impregnator to render it acid-soaked and draining the acid-soaked biomass to about 30% to 35% by weight solids; b) dewatering the acid-soaked biomass by drying or centrifugation to prevent compaction of the biomass and arrive at about 40% to 60% by weight solids; c) subjecting the acid-impregnated biomass to a first-stage hydrolysis reactor at a temperature of from 130° C. to 220° C. and discharging formed hydrolysate into a flash tank at about 120° C. to 140° C. to hydrolyze most of the remaining soluble oligosaccharides to monomeric sugars, and flashing remaining hydrolysate to a second flash tank at a lower temperature than the first flash tank-the second flash tank serving as a feed surge tank for a counter-current extractor; d) washing the hydrolysate, adjusting the pH of the sugar extract to about 5, and recovering more than 95% of the soluble sugars in the first-stage hydrolysate slurry by a counter-current extractor; e) subjecting remaining washed-first stage solids of pretreated biomass to a second-stage acid and metal salt impregnator and dewatering by drying or centrifugation to prevent compaction of biomass to arrive at 40% to 60% by weight solids; f) subjecting the acid and metal salt-impregnated biomass to a second-stage hydrolysis reactor at a temperature from 190° C. to 240° C. and discharging formed hydrolysate into a flash tank, at about 120° C. to 140° C. to hydrolyze most of the remaining soluble oligosaccharides to monomeric sugars and flashing remaining hydrolysate to a second flash tank at a lower temperature than the first flash tank, the second flash tank serving as a feed surge tank for second-stage fementors; g) cooling pH-adjusted extract from the counter-current extractor, feeding the extract to a first-stage fermentor and air sparging the first-stage fermentor at a rate sufficient to promote enough yeast growth to compensate for loss through second-stage fermentors; h) pH adjusting second-stage hydrolysate slurry to 4.5, cooling the slurry and adding it into the top of the first fermentor of a two-fermentor train in the second stage fermentors, pumping broth from the bottom of the first stage fermentors to the second stage fermentors while the yeast is in the growth phase for a period sufficient to consume over 95% of fermentable sugars; and i) recovering ethanol.

Described herein are improved methods of pretreating lignocellulosic biomass. One aspect of the invention relates to a two-stage pretreatment process comprising a relatively low severity steam treatment, a controlled pH pretreatment, or autohydrolysis, followed by hydrolysis with dilute acid or hot water at a relatively low temperature. In certain embodiments, the methods increase hemicellulose sugar yields, substrate digestibility, and suitability for fermentation as compared to steam explosion or acid hydrolysis alone. The two-stage pretreatment processes also employ few chemicals, minimizing the costs associated with pretreatment of lignocellulosic biomass. Moreover, the two-stage pretreatment process may expand the range of suitable feedstocks for bioethanol production.

The invention belongs to the fields of fermentation and production of microorganisms, and in particular relates to a method for improving the yield of trehalose in a yeast strain by a dry dehydration method. A method for preparing the trehalose comprises the following steps of: fermenting the yeast strain at the temperature of between 30 and 35 DEG C, performing enlargement culture, collecting thalli, performing extrusion dehydration, and performing forced air drying on the dehydrated thalli at the temperature of between 30 and 95 DEG C until the water content of the thalli is 5 to 25 percent; performing ultramicro crushing on the dried thalli to obtain wall breaking thalli powder; and dissolving the thalli powder in water, uniformly mixing, performing ultrafiltration by using an ultrafiltration membrane, and collecting filtration liquor to obtain a solution in which the trehalose is dissolved. By the method, the water content of the yeast thalli is gradually reduced through forced air drying, the yeast thalli is stimulated to synthesize the trehalose in large quantities under a dry environment, the accumulation amount of the trehalose in the yeast thalli is improved, and the content of the trehalose in a yeast cell can be improved by 85 percent.

Methods and systems are disclosed for the hydrolysis of mixed biomass. The methods include forming a mixture of at least two modified biomass feedstocks to achieve various benefits, such as maximizing sugar yields and minimizing the formation of degradation products.

It is intended to provide an agent for improving sugarcane Brix which increases the sugar productivity from pressed juice after harvesting and promotes ripening when applied to sugarcane and, therefore, by which a sugar yield comparable to sugarcane harvested at the best harvest season can be established even from sugarcane before reaching the harvest season, which comprises at least one member of the compounds represented by the following general formula (I)wherein A represents an oxygen atom, a sulfur atom, or a hydroxymethylene group; Y represents a carboxyl group, an alkyloxycarbonyl group, a halogenated methylsulfonylamino group or a diphenylmethylideneiminoxycarbonyl group, either in a free form or forming a salt; and R represents a halogen atom, an alkyl group, a hydroxy-, alkoxy- or alkoxyiminoalkyl group or a 4,6-dimethoxypyrimidin-2-yloxy group, or Y and R may be bonded together to form a group —C(═O)—O—CH(CH3)—.

The invention discloses a method for producing N-acetylglucosamine by jointly using glucose and xylose based on CRISPRi and belongs to the field of genetic engineering. Through the method, bacillus subtilis BSGNY-Pveg-g1mS-P[43]-GNA1 is used as a starting strain; dCas9 induced by xylose and three sgRNA expression fragments which respectively act on three genes including zwf, pfkA and g1mM are integrated on a genome; the strain is used for carrying out flask shaking fermentation; the yield of N-acetylglucosamine reaches up to 20.5g / L; and the yield of N-acetylglucosaminein per gram of glucose is 0.612g / g; meanwhile, the recombinant bacillus subtilis is capable of efficiently and jointly using glucose and xylose; the method lays the foundation for production of glucosamine through the modified bacillus subtilis in further metabolic engineering and the industrialization thereof.

The invention provides an organic fertilizer with high humic acid content and a preparation method thereof. The preparation method of the organic fertilizer with high humic acid content includes the following steps: preparing fermentative bacteria, wherein the fermentative bacteria include bacillus subtilis, bacillus megaterium and bacillus mucilaginosus; making the fermentative bacteria into fermentation microbial agent; filtering mud in a sugarhouse through a sulfurous method, and mixing with the fermentation microbial agent for fermentation to obtain the organic fertilizer with high humic acid content. Compared with the conventional organic fertilizer, the organic fertilizer obtained through the technical scheme provided by the invention is higher in humic acid conversion rate and humic acid content; the sulfurous method is fully utilized to filter mud in the sugarhouse, and resource utilization is realized in the cane sugar industry, and the problem of environment pollution troubling the sugarhouse, caused by the mud filtration with the sulfurous method in the sugarhouse is solved; wastes are turned to treasures, soil productivity is increased, the sugar yield is increased, cleaner production is realized, the environment is protected, and the sustainable development of the sugar industry is promoted.

Methods and systems are disclosed for the hydrolysis of mixed biomass. The methods include forming a mixture of at least two modified biomass feedstocks to achieve various benefits, such as maximizing sugar yields and minimizing the formation of degradation products.

The invention provides a method for improving sugar yield of lignocellulosic biomass after enzymolysis. The method comprises the following steps: (1) taking lignocellulosic biomass as a raw material; (2) mixing the lignocellulosic biomass, organic alkali and an organic solvent; (3) filling the mixed system with CO2 with certain pressure, and reacting for certain time; (4) releasing CO2, recycling the biomass by adopting an anti-solvent of cellulose; and (5) taking a certain amount of recycled lignocellulosic biomass, and adding a buffer solution and cellulase as well as xylanase for carrying out enzymolysis, thus obtaining a sugar solution. According to the method, under the existence of the organic alkali, hydroxide radicals in the lignocellulosic biomass and CO2 are subjected to reversible reaction, so that a crystal texture of the cellulose in the lignocellulosic biomass is damaged, and along with the conduction of the reaction, the lignocellulosic biomass is dissolved or subjected to swelling under the effect of the organic solvent, and the lignocellulosic biomass is subjected to the dissolving pretreatment; and the method has the advantages that the technology is simple, the cost of the solvent is low, the operation is convenient, the solvent can be recycled, the hydrolysis speed of the cellulose and the hemicellulase in the lignocellulose is high, and the sugar yield is high.