The invention discloses three-laminated composite board type full-nutritional rice seedling breeding bedding and a preparation method thereof and relates to rice seedling breeding bedding and a preparation method thereof. The problems that existing seedling breeding bedding is poor in water-retaining property, fertilizer efficiency in the later period of seedling breeding is poor, strength is low, and the existing seedling breeding bedding is not suitable for being transported and carried are solved. The seedling breeding bedding is divided into three layers from top to bottom, the middle layer is a core layer, and the surface layers are located on the two sides of the core layer. The preparation method comprises the following steps that (1), straw is cut into sections; (2), steaming and softening are conducted; (3), the straw is ground and kneaded to be in a fiber shape and is dried; (4), mixing is conducted; (5), paving is conducted; and (6), hot-pressing is conducted. According to the three-laminated composite board type full-nutritional rice seedling breeding bedding (as shown in figure 1) produced through the preparation method, when no water is absorbed by the three-laminated composite board type full-nutritional rice seedling breeding bedding, the static bending intensity is 21-22.8 MPa, and the internal bonding strength is 0.35-0.40 MPa; and the three-laminated composite board type full-nutritional rice seedling breeding bedding is high in strength, not prone to breakage or damage and easy to cut and can be manufactured to be in different shapes.