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Artificial nutrient medium for Phellinus linteus fluid culture and method for fermentation of Phellinus linteus polysaccharide

A technology for synthesizing culture medium and liquid culture, applied in the field of Phellinus linteus fermenting and producing Phellinus linteus polysaccharides, can solve the problem of no Phellinus linteus fully synthetic medium and the like, and achieve the effects of stable quality, rapid growth and high purity

Inactive Publication Date: 2009-01-21
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no literature and patent reports on Phellinus fully synthetic medium at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Prepare fermentation medium 1L described in the present invention, the concentration (g / L) of its each component is: glucose 50g, glutamic acid 0.8g, glutamine 0.8g, aspartic acid 0.8g, asparagine 0.8g glutamic acid 0.04g, tryptophan 0.04g, valine 0.04g, alanine 0.04g, leucine 0.04g, isoleucine 0.04g, phenylalanine 0.04g, methionine 0.04g, Proline 0.04g, Glycine 0.04g, Serine 0.04g, Lysine 0.04g, Cysteine ​​0.04g, Tyrosine 0.04g, Arginine 0.04g, Histidine 0.04g, MgSO 4 1.0g, KH 2 PO 4 1.0g, NaCl 0.85g, FeSO 4 0.05g, MnSO 4 7H 2 O 0.0383g, ZnSO 4 7H2O 0.0383g, CoCl 2 0.0043g, CuSO 4 0.0096g, CaCl 2 0.25g, VB 1 200ug,H 2 O 1.0L to adjust the pH to 6.0, divide the medium into 250mL Erlenmeyer flasks, the filling volume is 50mL, the sterilization temperature is 115°C, and the time is 30min.

[0015] Phellinus mycelium culture: inoculate 10% (V / V) Phellinus seed solution, cultivate at 25°C, rotate at 180rpm / min, sample every 24h, and centrifuge at 3500rpm fo...

Embodiment 2

[0017] Prepare 1L of fermentation medium described in the present invention, the concentration (g / L) of its each component is: glucose 70g, glutamic acid 0.5g, glutamine 0.5g, aspartic acid 0.5g, asparagine 0.5g, glutamic acid 0.04g, tryptophan 0.04g, valine 0.04g, alanine 0.04g, leucine 0.04g, isoleucine 0.04g, phenylalanine 0.04g, methionine 0.04g , proline 0.04g, glycine 0.04g, serine 0.04g, lysine 0.04g, cysteine ​​0.04g, tyrosine 0.04g, arginine 0.04g, histidine 0.04g, MgSO 4 1.0g, KH 2 PO 4 1.0g, NaCl 0.85g, FeSO 4 0.05g, MnSO 4 7H 2 O 0.0383g, ZnSO 4 7H2O 0.0383g, CoCl 2 0.0043g, CuSO 4 0.0096g, CaCl 2 0.25g, VB 1 200ug,H 2 O 1.0L to adjust the pH to 6.0, divide the medium into 250mL Erlenmeyer flasks, the filling volume is 50mL, the sterilization temperature is 115°C, and the time is 30min.

[0018] Phellinus mycelium culture: inoculate 10% (V / V) Phellinus seed solution, cultivate at 25°C, rotate at 180rpm / min, sample every 24h, and centrifuge at 3500r...

Embodiment 3

[0020] Prepare fermentation medium 1L described in the present invention, the concentration (g / L) of its each component is: glucose 50g, glutamic acid 0.8g, glutamine 0.8g, aspartic acid 0.8g, asparagine 0.8g, glutamic acid 0.04g, tryptophan 0.04g, valine 0.04g, alanine 0.04g, leucine 0.04g, isoleucine 0.04g, phenylalanine 0.04g, methionine 0.04g , proline 0.04g, glycine 0.04g, serine 0.04g, lysine 0.04g, cysteine ​​0.04g, tyrosine 0.04g, arginine 0.04g, histidine 0.04g, MgSO 4 1.0g, KH 2 PO 4 1.0g, NaCl 0.85g, FeSO 4 0.05g, MnSO 4 7H 2 O0.0383g, ZnSO 4 7H2O 0.0383g, CoCl 2 0.0043g, CuSO 4 0.0096g, CaCl 2 0.25g, VB 1 200ug,H 2 O1.0L to adjust the pH to 6.0, divide the medium into 250mL Erlenmeyer flasks, the filling volume is 50mL, the sterilization temperature is 115°C, and the time is 30min.

[0021] Phellinus mycelium culture: inoculate 10% (V / V) Phellinus seed solution, cultivate at 25°C, rotate at 180rpm / min, sample every 24h, and centrifuge at 3500rpm ...

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Abstract

The invention provides a synthetic medium for culturing a phellinus igniarius liquid. The synthetic medium takes glucose as a carbon source and 20 kinds of amino acid as a nitrogen source, and consists of inorganic salt and vitamin. The ratio of carbon to nitrogen is designed as 50 to 1; and the specific concentration g / l is as follows: the glucose: 30 to 80 grams; glutamic acid: 0.1 to 1.5 grams; glutamine: 0.1 to 1.5 grams; aspartic acid: 0.1 to 1.5 grams; asparagines: 0.1 to 1.5 grams; glutamic acid: 0.01 to 0.1 gram; tryptophan: 0.01 to 0.1 gram; valine: 0.01 to 0.1 gram; alanine: 0.01 to 0.1 gram; etc. During the whole culture process of the synthetic medium, phellinus igniarius mycelium is quick in growth; the yield of the phellinus igniarius mycelium can reach 20 grams per liter to the highest degree; simultaneously no agricultural and sideline product is introduced as the carbon source and the nitrogen source; substances such as proteins in the medium, medium compositions and so on are not carried; phellinus igniarius exopolysaccharide produced through fermentation has high purity and stable quality; and the yield of phellinus igniarius polysaccharide can reach 0.198 gram per liter to the highest degree. The synthetic medium has the advantages of clear compositions, stable quality and so on, and can be used for mass culture of the phellinus igniarius liquid and research of the regulation and control rule of polysaccharide anabolism.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a fully synthetic medium used for the liquid cultivation of the medicinal fungus Phellinus phellidis and a method for producing Phellinus polysaccharide by fermenting the Phellinus phellidis using the medium. Background technique [0002] Phellinus igniarius, also known as Phellinus igniarius, belongs to Basidiomycetes, Phellinus igniarius, parasitic on mulberry trees, is a rare edible and medicinal fungus, the main active ingredient of Phellinus igniarius is Phellinus polysaccharide , can improve the body's immunity, has significant anti-tumor activity, and is of great development value. [0003] Due to the complexity and particularity of the physiological ecology of Phellinus fungus, the number of Phellinus fruiting bodies is scarce. Large-scale production of Phellinus mycelium and its active polysaccharides by liquid submerged culture technology is an effectiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12P19/04C12R1/645
Inventor 邹祥郭霞孙敏
Owner SOUTHWEST UNIVERSITY
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