Novel method for saccharification of ligno-cellulose

A technology of lignocellulose and a new method, which is applied in the field of cellulose hydrolysis and lignocellulose processing to make sugar, can solve the problems of increased energy consumption and production cost, increased maintenance and maintenance costs, high requirements on corrosion resistance, etc. Accessibility and site of action, saving operating costs and time, eliminating individual differences

Inactive Publication Date: 2009-05-20
CHINA PETROLEUM & CHEM CORP
View PDF8 Cites 30 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creation of highly pure glucohydroxypropyltripeptides (GHTP), an important ingredient found naturally throughout nature. These GHPTFTs have been shown to improve their ability to break down plant biomass into sugars like sucrose or starch. By combining these two types of molecules together they form more stable solutions than just mixing them separately without adding other substances such as water. Overall, this new approach makes efficient utilization of resources while minimizing environmental impact has potential benefits across industries including food processing, chemical manufacturing, paper making, agricultural fertilizers, animal feed supplements, cosmetic products, pharmaceuticals, detergents, plastic compositions, etc., resulting improved economic performance and reduced operational expenses associated therewith.

Problems solved by technology

Technological Problem addressed in this patents relates to finding an efficient way to break down biopolysulfonate into sugars while minimizing damage during processing of carbohydrils like hemi-cellulosilage.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel method for saccharification of ligno-cellulose
  • Novel method for saccharification of ligno-cellulose
  • Novel method for saccharification of ligno-cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] dry corn stover with OH - The NaOH solution with a concentration of 0.01mol / L is mixed and soaked according to the ratio of material to liquid 1:5 (kg:L) for 8 hours, and then added to a large twin-screw extruder (Jinan Dayi Extrusion Machinery Co., Ltd.) for extrusion treatment. The operating parameters are: Sleeve temperature 90°C, screw speed 20(r min -1 ), with a moisture content of 20%, steamed and exploded once.

[0070] Selection of cellulase and xylosidase for compounding:

[0071] A, cellulase Accellerase 1000 (purchased from Genencor International Company)

[0072] The results of enzyme activity determination ( / mL cellulase): filter paper enzyme activity 93FPIU, CMC enzyme activity 7.38IU, β-glucosidase 1632.9IU;

[0073] B. Cellulase ZSL-1300 (Shandong Zesheng Biotechnology Company)

[0074] The results of enzyme activity determination ( / mL cellulase): filter paper enzyme activity 77 FPIU, CMC enzyme activity 17.35 IU, β-glucosidase 1356.5 IU;

[0075] C, ...

Embodiment 2

[0080] The bagasse and the HCl solution with a H+ concentration of 0.01mol / L were mixed and soaked for 10 hours according to the ratio of material to liquid 1:15 (kg:L), and then added to a DS322 II single-screw extruder (Jinan Saixin Extrusion Machinery Co., Ltd.) Extrusion puffing treatment, the operating parameters are: screw speed 180 (r min -1 ), moisture content 70%, steamed and exploded 3 times.

[0081] Select cellulase and xylase for compounding:

[0082] A, cellulase Accellerase 1000 (purchased from Genencor International Company)

[0083] The results of enzyme activity determination ( / mL cellulase): filter paper enzyme activity 93 FPIU, CMC enzyme activity 7.38 IU, β-glucosidase 1632.9 IU;

[0084] B, cellulase (purchased from Wuxi Gaorunjie Biological Engineering Co., Ltd.)

[0085] The results of enzyme activity determination ( / g cellulase): filter paper enzyme activity 22 FPIU, CMC enzyme activity 7.8 IU, β-glucosidase 919.5 IU;

[0086] C, cellulase EN0029 (...

Embodiment 3

[0093] Add the dry material of corn stalks directly into the DS322 II single-screw extruder (Jinan Saixin Extrusion Machinery Co., Ltd.), add water for extruding and extruding, operating parameters: screw speed 180 (r min -1 ), moisture content 70%, steamed and exploded 3 times.

[0094] Select cellulase and xylase for compounding:

[0095] A, cellulase Accellerase 1000 (purchased from Genencor International Corporation)

[0096] The results of enzyme activity determination ( / mL cellulase): filter paper enzyme activity 93FPIU, CMC enzyme activity 7.38 IU, β-glucosidase 1632.9 IU;

[0097] B, Cellulase EN0029 (purchased from Shanghai Sculler Biotechnology Co., Ltd.)

[0098] The results of enzyme activity determination ( / g cellulase): filter paper enzyme activity 52 FPIU, CMC enzyme activity 105 IU, β-glucosidase 910.5 IU;

[0099] C, xylanase (purchased from Shandong Zesheng Biotechnology Company), the enzyme activity is 964IU / mL.

[0100] Above-mentioned three kinds of en...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a new method for processing lignocellulose and obtaining sugar, which is characterized by comprising expelling-expansion pretreatment, cellulase compounding and enzymolysis saccharification, and a sugar solution collection process; at least two types of cellulase are mixed with xylanase for obtaining compound cellulase, and then added into lignocellulose raw materials undertaking expelling-expansion pretreatment, finally total sugar solution is obtained by one-step enzymolysis saccharification. By using the process that the expelling-expansion pretreatment is combined with the one-step enzymolysis of compound enzyme, the hydrolysis sugar yield of the lignocellulose raw materials can be up to more than 90 percent. The method has the advantages of simple and environmental-protection pretreatment, full use of enzyme, mild enzyme hydrolysis reaction conditions, simple operation, and high enzymolysis efficiency, produces no inhibition to the follow-up fermentation, can be comprehensively utilized, and causes no environmental pollution, etc.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner CHINA PETROLEUM & CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap