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Method for improving cellulase hydrolysis sugar field

A cellulase and cellulose technology, applied in fermentation and other directions, can solve the problems of high purification and recycling costs and high solvent costs

Inactive Publication Date: 2015-09-30
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method solves the problems of high cost of solvent and high cost of purification, recovery and utilization in the current method of producing cellulose dissolution pretreatment,

Method used

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  • Method for improving cellulase hydrolysis sugar field
  • Method for improving cellulase hydrolysis sugar field
  • Method for improving cellulase hydrolysis sugar field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] figure 1 It is a schematic flow chart of a method for improving the sugar production rate of cellulose enzymatic hydrolysis provided by the present invention. Such as figure 1 As shown, it is carried out according to the following steps:

[0060] 1. Using cellulose as raw material;

[0061] 2. Will be available with CO 2 One or two or more compound raw materials in the Switchable ionic compounds (Switchable ionic compounds) are mixed with cellulose to form a pre-dissolved system;

[0062] or will be available with CO 2 One or two or more compound raw materials in the switch-type ionic compound formed by reaction are mixed with cellulose and an organic solvent with good swelling effect on cellulose to form a pre-dissolved system;

[0063] or CO 2 One or more of Switchable ionic compounds are mixed with cellulose to form a pre-dissolved system;

[0064] or CO 2 One or two or more of the switch-type ionic compounds are mixed with cellulose and an organic solvent ...

Embodiment 2

[0070] Mix methanol (0.039 mol, 0.8 g), tetramethylguanidine (0.039 mol, 4.5 g), DMSO (12.2 g), and 2.0 g of corn stalk cellulose, add to the autoclave, close the lid tightly, and pass in CO 2 , maintain the pressure at 0.2MPa, heat to 50°C, and dissolve for 60 minutes. cool down, releasing CO 2 , Open the reaction kettle, add 100ml of anhydrous methanol to the system under stirring conditions, regenerate the dissolved cellulose, filter, and collect the filtrate. Then the filter residue was washed with 2*100ml of anhydrous methanol, each time for 1h, to completely remove the solvent, and then dried to obtain dissolved pretreated cellulose. Filtrate, collect filter residue, and freeze-dry to obtain regenerated cellulose. Using regenerated cellulose as raw material, add acetic acid-sodium acetate buffer solution, the solid-to-liquid ratio is 1:10, the amount of Trichoderma viride is 15 FPU / g, the shaking table speed is 100 rpm, and the enzymatic hydrolysis condition is pH4.5. ...

Embodiment 3

[0072] Ethylene glycol (0.018 moles, 1.14 grams), tetramethylguanidine (0.036 moles, 4.14 grams), DMSO (12.7 grams), and 1.0 grams of microcrystalline cellulose were mixed, added to the autoclave, and the lid was tightly closed. Into CO 2 , maintain the pressure at 0.2MPa, heat to 50°C, and dissolve for 60 minutes. cool down, releasing CO 2 , Open the reaction kettle, add 100ml of anhydrous methanol to the system under stirring conditions, regenerate the dissolved cellulose, filter, and collect the filtrate. Then the filter residue was washed with 2*100ml of anhydrous methanol, each time for 1h, to completely remove the solvent, and then dried to obtain dissolved pretreated cellulose. Filtrate, collect filter residue, and freeze-dry to obtain regenerated cellulose. Regenerated cellulose was used as raw material, citric acid-sodium citrate buffer solution was added, the solid-to-liquid ratio was 1:20, the amount of Trichoderma viride was 20 FPU / g, the shaking table speed was...

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Abstract

The invention provides a method for improving cellulase hydrolysis sugar field. The method comprises the following steps: (1) taking cellulose as a raw material; (2) dissolving the cellulose in a CO2 switch-type ionic compound or a mixed solvent consisting of the CO2 switch-type ionic compound and an organic solvent, thereby obtaining a cellulose-containing solution; (3) adding an anti-solvent into the cellulose solution, regenerating, filtering and separating the dissolved cellulose, thereby obtaining the pretreated cellulose material; and (4) taking a certain amount of regenerated cellulose, adding a buffer solution and cellulase for performing enzymolysis, thereby obtaining the glucose solution. The CO2 switch-type ionic compound or the mixed solvent consisting of the CO2 switch-type ionic compound and the organic solvent serves as a solvent, the cellulose is subjected to dissolving pretreatment, and the method has the advantages that the process is simple, the solvent cost is low, the operation is convenient, the solvent can be recycled, the cellulase hydrolysis speed is high, the sugar yield is high and the like.

Description

technical field [0001] The present invention relates to a kind of preparation using cellulose as raw material, by using CO 2 Switching ionic compounds or CO 2 The invention relates to a method for pretreating cellulose with a mixed solvent composed of a switch-type ionic compound and an organic solvent, and preparing glucose through enzymatic hydrolysis, which belongs to the field of biomass utilization and biochemical products. Background technique [0002] With the increasing shortage of non-renewable resources such as coal, oil, and natural gas, natural cellulose, as the most abundant renewable resource in nature, has attracted more and more attention. Since cellulose is the most widely distributed and most abundant polysaccharide in nature, it is the main component of plant cell walls. Cotton, flax, taro and jute contain a lot of high-quality cellulose. The cellulose content of wood is about 50%, and the cellulose content of cotton is higher, reaching 90%. Cellulose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 谢海波赵宗保沈宏伟
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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