Method for improving cellulase hydrolysis sugar field

A cellulase and cellulose technology, applied in fermentation and other directions, can solve the problems of high purification and recycling costs and high solvent costs
CN104946701AInactive Publication Date: 2015-09-30DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2015-09-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for improving cellulase hydrolysis sugar field. The method comprises the following steps: (1) taking cellulose as a raw material; (2) dissolving the cellulose in a CO2 switch-type ionic compound or a mixed solvent consisting of the CO2 switch-type ionic compound and an organic solvent, thereby obtaining a cellulose-containing solution; (3) adding an anti-solvent into the cellulose solution, regenerating, filtering and separating the dissolved cellulose, thereby obtaining the pretreated cellulose material; and (4) taking a certain amount of regenerated cellulose, adding a buffer solution and cellulase for performing enzymolysis, thereby obtaining the glucose solution. The CO2 switch-type ionic compound or the mixed solvent consisting of the CO2 switch-type ionic compound and the organic solvent serves as a solvent, the cellulose is subjected to dissolving pretreatment, and the method has the advantages that the process is simple, the solvent cost is low, the operation is convenient, the solvent can be recycled, the cellulase hydrolysis speed is high, the sugar yield is high and the like.
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Description

technical field

[0001] The present invention relates to a kind of preparation using cellulose as raw material, by using CO 2 Switching ionic compounds or CO 2 The invention relates to a method for pretreating cellulose with a mixed solvent composed of a switch-type ionic compound and an organic solvent, and preparing glucose through enzymatic hydrolysis, which belongs to the field of biomass utilization and biochemical products. Background technique

[0002] With the increasing shortage of non-renewable resources such as coal, oil, and natural gas, natural cellulose, as the most abundant renewable resource in nature, has attracted more and more attention. Since cellulose is the most widely distributed and most abundant polysaccharide in nature, it is the main component of plant cell walls. Cotton, flax, taro and jute contain a lot of high-quality cellulose. The cellulose content of wood is about 50%, and the cellulose content of cotton is higher, reaching 90%. Cellulose ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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