Method for improving sugar yield of lignocellulosic biomass after enzymolysis

A technology of lignocellulose and cellulase, applied in the direction of fermentation, etc., can solve the problems of high cost of purification and recycling, high cost of solvent, etc., and achieve the effect of convenient operation, low cost of solvent and high sugar yield

Inactive Publication Date: 2016-04-20
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method solves the problems of high solvent cost and high cost of purification, recycling and utilization in the current lignocellulose pretreatment method.

Method used

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  • Method for improving sugar yield of lignocellulosic biomass after enzymolysis
  • Method for improving sugar yield of lignocellulosic biomass after enzymolysis
  • Method for improving sugar yield of lignocellulosic biomass after enzymolysis

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Experimental program
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Embodiment 1

[0048] A method for improving the sugar production rate of lignocellulosic biomass enzymatic hydrolysis, characterized in that: it is carried out according to the following steps:

[0049] 1) Using lignocellulosic biomass as raw material;

[0050] 2) mixing lignocellulosic biomass, an organic base and an organic solvent;

[0051] 3) Fill the mixed system with a certain pressure of CO 2 , react at a certain temperature for a certain time;

[0052] 4) Release CO 2 , adding an anti-solvent to the system, the biomass is regenerated with the anti-solvent of cellulose, and then filtered and separated to obtain the pretreated lignocellulosic material;

[0053] 5) Taking a certain amount of regenerated lignocellulosic biomass, adding a buffer solution, cellulase and xylanase to carry out enzymatic hydrolysis to obtain a sugar solution.

[0054] See attached for details figure 1 .

Embodiment 2

[0056] Add corn stalk lignocellulose (2.0 grams, physical size: 1 mm, moisture content2 , maintain the pressure at 0.5MPa, heat to 100°C, and dissolve for 60 minutes. cool down, releasing CO 2 , Open the reaction kettle, add 100 ml of anhydrous methanol to the system under agitation to regenerate the dissolved cellulose, filter, and collect the filtrate. Then, the filter residue was washed with 2*100 ml of anhydrous methanol, each time for 1 h, to completely remove the solvent, and then dried to obtain dissolved and pretreated lignocellulose. Filtrate, collect filter residue, and freeze-dry to obtain regenerated cellulose. Using regenerated cellulose as raw material, add acetic acid-sodium acetate buffer solution, the solid-liquid ratio is 1:10, the addition amount of Trichoderma viride is 15 FPU / g, and the ratio of 0.01 g / g substrate is added to xylanase. Shaking table speed: 100 rpm, enzymolysis conditions are pH 4.5, temperature 50°C, and enzymolysis time is 24 hours. Gl...

Embodiment 3

[0058] Wheatgrass lignocellulose (2.0 g, physical size: 0.1 mm, water content 2 , maintain the pressure at 2MPa, heat to 80°C, and dissolve for 180 minutes. cool down, releasing CO 2 , open the reaction kettle, and add 100 milliliters of absolute ethanol to the system under stirring conditions to regenerate the dissolved lignocellulose, filter, and collect the filtrate. Then the filter residue was washed with 2*100 ml of absolute ethanol for 1 hour each time to completely remove the solvent, and then dried to obtain dissolved and pretreated lignocellulose. Filtrate, collect filter residue, and freeze-dry to obtain regenerated cellulose. Using regenerated lignocellulose as raw material, add citric acid-sodium citrate buffer solution, the solid-liquid ratio is 1:20, the addition amount of Trichoderma reesei is 20 FPU / g, and the ratio of 0.03 g / g substrate is added to xylanase . Shaking table speed: 200 rev / min, enzymolysis condition is pH5.5, temperature 50°C, enzymolysis tim...

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Abstract

The invention provides a method for improving sugar yield of lignocellulosic biomass after enzymolysis. The method comprises the following steps: (1) taking lignocellulosic biomass as a raw material; (2) mixing the lignocellulosic biomass, organic alkali and an organic solvent; (3) filling the mixed system with CO2 with certain pressure, and reacting for certain time; (4) releasing CO2, recycling the biomass by adopting an anti-solvent of cellulose; and (5) taking a certain amount of recycled lignocellulosic biomass, and adding a buffer solution and cellulase as well as xylanase for carrying out enzymolysis, thus obtaining a sugar solution. According to the method, under the existence of the organic alkali, hydroxide radicals in the lignocellulosic biomass and CO2 are subjected to reversible reaction, so that a crystal texture of the cellulose in the lignocellulosic biomass is damaged, and along with the conduction of the reaction, the lignocellulosic biomass is dissolved or subjected to swelling under the effect of the organic solvent, and the lignocellulosic biomass is subjected to the dissolving pretreatment; and the method has the advantages that the technology is simple, the cost of the solvent is low, the operation is convenient, the solvent can be recycled, the hydrolysis speed of the cellulose and the hemicellulase in the lignocellulose is high, and the sugar yield is high.

Description

technical field [0001] The invention relates to a method that utilizes lignocellulose as a raw material, and utilizes the presence of an organic base to combine the hydroxyl groups in the lignocellulose with CO 2 The reversible reaction regulates the crystal structure of cellulose in lignocellulose, pretreats lignocellulose, and prepares sugar by enzymatic hydrolysis, which belongs to the field of biomass utilization and biochemical products Background technique [0002] With the increasing shortage of non-renewable resources such as coal, oil, and natural gas, natural lignocellulosic biomass, as the most abundant renewable resource in nature, has attracted more and more attention. Lignocellulosic biomass is mainly composed of cellulose (35-50%), hemicellulose (20-40%), and lignin (15-25%), and the relative content of each component varies with the type of biomass and the place of origin. change with changes. Among them, cellulose is a complex polysaccharide with 8,000 to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 谢海波赵宗保于雪
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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