Pretreatment method capable of improving saccharification efficiency of lignocellulose

A technology of lignocellulose and saccharification efficiency, applied in the field of biofuel preparation, can solve the problems of high processing temperature and low yield of reducing sugar, and achieve the effect of simple processing process, low equipment requirements and high enzymatic saccharification efficiency

Active Publication Date: 2020-02-07
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it still has the technical problems of high processing temperature and low reducing sugar yield, which needs to be further improved

Method used

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  • Pretreatment method capable of improving saccharification efficiency of lignocellulose
  • Pretreatment method capable of improving saccharification efficiency of lignocellulose
  • Pretreatment method capable of improving saccharification efficiency of lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Wash the rice straw with a particle size of 180-400 μm in clear water, and dry it at 55° C. to constant weight;

[0032] (2) After mixing 5% (W / W) distilled water with 0.5:1:1 mixed oxalic acid / glycerol / choline chloride and sealing it, place it in a drying oven at 100°C until it is evenly mixed into a transparent liquid reserve;

[0033] (3) Mix and oscillate 10% (W / W) of rice straw with the new organic solvent in (2) in a shaker at 80°C, 100°C, and 120°C for 4 hours for pretreatment, and then wash the filter residue until the lotion is neutral. Dry the filter residue to obtain three pretreated samples, 1;

[0034] (4) After mixing 22 FPU / g substrate cellulase and 25 mg / mL pretreated sample with citrate buffer (pH 4.80, 0.1 mol / L), enzymolyze 22 at 100 rpm / min at 50°C ~72h, sampling every 24 hours to measure the amount of reducing sugar produced.

[0035] After being treated in this embodiment, the reducing sugar content released by rice straw can be increased fr...

Embodiment 2

[0037] (1) Wash the rice straw with a particle size of 180-400 μm in clear water, and dry it at 55° C. to constant weight;

[0038] (2) With 5% (W / W) distilled water and 0.25:1:1, 0.5:1:1, 0.25:1:1.5, 0.5:1:1.5 oxalic acid / glycerol / choline chloride After mixing and sealing, put it in a drying oven at 100°C, and wait until it becomes a transparent liquid for later use;

[0039] (3) Mix and oscillate 10% (W / W) of rice straw with the new organic solvent in (2) for pretreatment in a shaker at 120°C for 4 hours, then wash the filter residue until the washing solution is neutral, and dry the filter residue to obtain 4 A pretreatment sample, 2;

[0040] (4) After mixing 22 FPU / g substrate cellulase and 25 mg / mL pretreated sample with citrate buffer (pH 4.80, 0.1 mol / L), enzymolyze 22 at 100 rpm / min at 50°C ~72h, sampling every 24 hours to measure the amount of reducing sugar produced.

[0041] After the treatment in this example, the content of reducing sugar released from rice st...

Embodiment 3

[0043] (1) Wash the rice straw with a particle size of 180-400 μm in clear water, and dry it at 55° C. to constant weight;

[0044] (2) After mixing 5% (W / W) distilled water with 0.25:1:1 mixed oxalic acid / glycerol / choline chloride and sealing it, place it in a drying oven at 100°C until it is evenly mixed into a transparent liquid reserve;

[0045] (3) Mix and oscillate 10% (W / W) of rice straw with the new organic solvent in (2) in a shaker at 120°C for 2, 4, 6, 8, and 10 hours for pretreatment, and then wash the filter residue into the lotion properties, drying the filter residue, and obtaining 5 kinds of pretreated samples 3;

[0046] (4) After mixing 22 FPU / g substrate cellulase and 25 mg / mL pretreated sample with citrate buffer (pH 4.80, 0.1 mol / L), enzymolyze 22 at 100 rpm / min at 50°C ~72h, sampling and measuring sugar production every 24 hours.

[0047] After being treated in this embodiment, the reducing sugar content released by rice stalks increased from 350.661mg...

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Abstract

The invention discloses a pretreatment method capable of improving saccharification efficiency of lignocellulose. According to the method, ethanedioic acid, choline chloride and glycerol are added towater for reaction to obtain a modified deep-eutectic solvent (DES), and a lignocellulose raw material is pretreated with the modified DES. The method can realize pretreatment of the lignocellulose under a lower-temperature condition, is more beneficial to improvement of the saccharification efficiency of the lignocellulose and can substantially increase the total quantity of carbohydrates released in a lignocellulose hydrolytic process.

Description

technical field [0001] The invention relates to a lignocellulose pretreatment method, in particular to a method for pretreating lignocellulose with a novel ternary modified deep eutectic solvent of oxalic acid / glycerol / choline chloride to improve the efficiency of enzymatic hydrolysis and saccharification The method belongs to the technical field of biofuel preparation. Background technique [0002] Lignocellulosic biomass such as agricultural and forestry crops and energy crops is environmentally friendly, renewable, large in production, and rich in carbohydrates. Making full use of this new energy to produce biofuels can greatly reduce the use of fossil fuels. The structure of lignocellulose is mainly composed of cellulose, hemicellulose and lignin. The three are closely intertwined through hydrogen bonds, covalent bonds and non-covalent bonds to form a strong protective umbrella, which enhances the enzymatic hydrolysis and saccharification of lignocellulose. resistance i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02D21C3/20
CPCC12P19/02C12P19/14C12P2201/00D21C3/20
Inventor 陈跃辉田文静贺倩妮陈玥如
Owner XIANGTAN UNIV
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