111results about How to "Add binding sites" patented technology

The invention discloses an aluminum-based copper-plated graphene film composite material with high heat-conducting property and a preparation method thereof. The preparation method comprises the following steps of: de-oiling an aluminum substrate sample for 3min in a 65 DEG C de-oiling solution and then cleaning by clear water; carrying out bright dipping on the de-oiled sample for 2min in a 25 DEG C bright dipping solution and then cleaning by clear water; carrying out zinc dipping on the sample (subjected to the bright dipping) for 2min in a 25 DEG C primary zinc dipping solution and then cleaning by clear water; carrying out zinc dipping on the sample (subjected to the primary zinc dipping) for 2min in a 25 DEG C secondary zinc dipping solution and then cleaning by clear water; plating the sample (subjected to the secondary zinc dipping) for 5min by direct current in a 25 DEG C priming copper plating solution, wherein the current density is 1-4A/dm2, the polar distance is 5cm, and then cleaning by clear water; plating the sample (subjected to the copper plating) for 20-40min by directional pulse in a 25 DEG C composite copper plating graphene plating solution and then cleaning by clear water, wherein the current density is 1-4A/dm2 and the polar distance is 5cm. The preparation method has the following advantages that the requirement on equipment is low, the operation is convenient, the process route is clear and the thickness of the film is controllable.

The invention relates to a preparation method of a molecular imprinting polymer. The preparation method comprises the following steps: dissolving dimethyl methylphosphonate (DMMP), a functional monomer A and a functional monomer B in a pore former, performing ultrasonic degassing for at least 5 minutes, placing the mixture in a refrigerator for above 8 hours to obtain a template molecule-monomer complex, adding a crosslinking agent in the template molecule-monomer complex, performing ultrasonic vibration for at least 30 minutes, introducing nitrogen for at least 15 minutes while adding an initiator, sealing, performing thermal polymerization in a suspension prepared from polyvinyl alcohol, cooling to the room temperature to separate out precipitates, centrifuging, performing Soxhlet extraction on the obtained polymer to ensure that the polymer has no imprinted molecules, washing microspheres with chloroform to remove residual methanol and acetic acid, and drying the microspheres in vacuum to reach a constant weight and obtain DMMP molecular imprinting polymer microspheres. The DMMP molecular imprinting polymer microspheres prepared by the method have the advantages of obviously higher adsorption capacity, larger specific surface area and good selectivity.

The invention discloses a temperature-sensitive hepatic cell culture support material and a preparation method thereof, and relates to an intelligent tissue engineering support material and technology, in particular to a temperature-sensitive hepatic cell culture support material, a method and technology. The support material is characterized in that: the surface of the material is relatively lyophobic at the temperature of 37 DEG C and is suitable for cell adherence/spreading and cell propagation, but the surface is hydrophilic at the temperature of 32 DEG C, so cells are desorbed from the surface automatically. The preparation method of the cell culture support material comprises the following steps of: (1) surface cleaning; (2) the temperature-sensitive carboxylation modification of a TCPS surface; (3) the preparation of amido lactobionic acid (L-NH2); and (4) the saccharifying modification of the TCPS surface. Through the preparation method, the biocompatibility and cell-bound loci of the support material are enhanced, the adsorption rate of the cells on the material surface is increased, and the intelligent support material which is suitable for hepatic tissue engineering is obtained.

The invention provides an indinavir ketone derivative with the alkyl-amino side chain, which is a change for the connecting chain between the benzene ring and tertiary amine nitrogen in the molecule of the phenoxy indinavir ketone compound; the distance between the benzene ring and tertiary amine nitrogen is extended; the connecting chain of the original one-carbon unit (methylene) is changed into a connecting chain with a 2 to 4 carbon atoms, or a connecting chain with the carbonyl, so as to get a series of novel phenoxy indinavir ketone derivative with the alkyl-amino side chain on the benzene ring. The novel synthesized phenoxy indinavir ketone derivative has the strong inhibitory activity for AChE and the weak inhibition for BuChE; the selectivity is high; the compound can be used in the drug for the treatment of the disease of Alzheimer. The method of the invention is reasonable in design, simple in operation and strong in practicality. In the invention, the structure of the indinavir ketone derivative with the alkyl-amino side chain is shown in the formula (see figure).

The invention provides a hollow braided pipe modification method, which comprises: modifying a hollow braided pipe by using a modification solution containing a polyphenol compound and a cross-linkedpolymer. The present invention further provides a hollow fiber film preparation method, which comprises: A, modifying the hollow braided pipe as a support material by using the modification method; B,carrying out mineralization treatment on the hollow braided pipe treated in the step A with a salt solution; and C, carrying out surface coating on the hollow braided pipe treated in the step B witha film forming liquid. According to the present invention, the preparation process is simple, the used raw materials are cheap, and the method is suitable for industrial production.

The invention relates to a method for preparing an in-situ tissue engineering blood vessel by a composite process. According to the invention, a three-layer composite intravascular stent which is similar to a natural vascular structure and is used for in-situ tissue engineering is prepared by combining rapid prototyping, electrostatic spinning and wet spinning processes; wherein the inner layer isa tubular stent provided with an axial groove by utilizing 3D printing and electrostatic spinning technologies; a middle layer is a tubular stent which is prepared by wet spinning and is provided with circumferentially arranged fibers; and the outer layer is the tubular stent which is prepared by the coatable inner layer obtained by electrostatic spinning and the disordered fibers at the middle layer. Through heparinization, the vascular endothelial cell growth factor is loaded, so that cells can be collected in a host, the infection opportunity of in-vitro culture is reduced, the preparationof in-situ tissue engineering blood vessels is realized, and the method has a wide prospect in the field of tissue engineering blood vessels.

The invention discloses a preparation method of an edible nanofiber preservative film. The preparation method comprises the following steps: (1) fully dissolving pullulan in ethyl alcohol with the volume fraction of 90%-95% at room temperature to enable the mass concentration of pullulan to be 16%-18%, and after fully dissolving pullulan, adding carboxymethyl cellulose to enable the mass concentration of the mixed solution to be 1%-2%, so as to obtain a basic spinning solution; (2) adding tea polyphenol and sodium citrate into the basic spinning solution to enable the mass concentrations of tea polyphenol and sodium citrate to be 0.5%-1% and 0.3%-0.5% respectively, fully stirring until uniformly dissolved, and leaving to stand until air bubbles completely disappear, so as to obtain a spinning solution; (3) placing the spinning solution on an electrostatic spinning machine for spinning, so as to obtain the edible nanofiber preservative film in the form of non-woven fabric. Compared with the coating method, the preparation method has the advantages that the prepared preservative film is good in the preservation effect, the raw material usage amount is small, and the prepared preservative film is lighter.

The invention discloses a method for preparing self-repairing hydrogel by guiding polymerization through a natural polymer template. The method comprises the following steps: taking a linear-chain natural polymer containing aldehyde groups as a template, fixing monomeric molecules containing amino groups on the natural polymer template through the action of Schiff base under alkaline conditions, and then, performing free radical polymerization to prepare polymer hydrogel with high rebound resilience and self-repairing performance. By virtue of existence of the natural linear-chain polymer template in the method disclosed by the invention, polymerization of monomers can be effectively guided, and dynamic chemical cross-linking sites are increased, thereby improving the mechanical strength and the self-repairing capability of the hydrogel. The hydrogel prepared by the method disclosed by the invention has broad application prospects in the related fields of artificial tissues and biomedicine.

The invention relates to a method for improving the enzymolysis saccharifying efficiency of hemp straw. The method is mainly characterized in that before enzymolysis saccharifying of hemp straw, the following pretreatment is performed: (1) crushing hemp straw, performing high-temperature sterilization, then inoculating white rot fungi into the crushed straw, and performing culture for 7-28 days toobtain biologically pre-treated hemp straw; and (2) mixing the biologically pre-treated hemp straw with a low-concentration alkali and hydrogen peroxide mixed solution, and performing treatment for 20-30 h at 40-60 DEG C to obtain pre-treated hemp straw, wherein the mass concentration of alkali in the mixed solution is 0.5-5%, and the volume concentration of hydrogen peroxide is 2.5-3.5%. Beforealkali oxygen treatment of hemp, biological pretreatment is performed at first, so that the consumption and the concentration of alkali in the alkali oxygen treatment process are reduced, the treatment time is shortened, the treatment efficiency is improved, and the pretreatment effect is improved.

The invention discloses a preparation method of a fuel cell membrane electrode CCM. The method comprises the following steps: preparing a hydrophilic catalyst, a perfluorosulfonic acid resin solution and a dispersing agent into a slurry with uniform components in a dispersing manner, and transferring the slurry to two sides of a proton exchange membrane to obtain the fuel cell membrane electrode CCM, wherein the EW value of the proton exchange membrane is 700 to 900; and the EW value of the perfluorinated sulfonic acid resin is 700 to 800; The hydrophilic catalyst and a short-side-chain low-EW-value resin are adopted, binding sites of water are increased so that the affinity of the catalyst layer to water is improved, a rich proton transmission network structure is constructed, the proton conduction rate of the catalyst layer is increased, the proton exchange membrane with a high water conduction capacity is adopted, the diffusion capacity of water generated by a cathode to an anode is enhanced, and a self water balance capability of the MEA under the condition that external humidification is not performed is realized. The whole preparation process does not introduce additional substances, does not need to change a membrane electrode preparation process flow, and is easy to popularize and apply.

The invention relates to a method for detecting a cancer marker MicroRNA based on a three-dimensional graphene biosensor. The three-dimensional graphene biosensor consists of a glass substrate and a three-dimensional graphene layer. Two sides of the upper surface of the glass substrate are provided with indium tin oxide (ITO), and the upper surface of part of indium tin oxide on both sides are covered with the three-dimensional graphene layer. The part uncovered with the three-dimensional graphene on one side serves as a source electrode, and the part uncovered with the three-dimensional graphene on the other side serves as a drain electrode. The method of the invention requires no labeling, is simple to operate and convenient to use, has higher selectivity and specificity under lower usevoltage, and has good safety.

The invention discloses an L-Glutamic acid (L-Glu) detection method and sensor based on a molecularly imprinted polymer (MIP) membrane modified electrode. The method comprises the following steps: preparation of an MWCNTs dispersion solution, preparation of an electropolymerization base solution, preparation of the MIP membrane modified electrode, L-Glu detection, and the like. Results show that the MIP/MWCNTs/GCE has sensitive L-Glu identification characteristics; and when the L-Glu concentration ranges from 1.00 x 10<-8>mol/L to 1.00 x 10<-5>mol/L, a good linear relationship (R<2> = 0.9923)between peak currents and the concentration is achieved, and the lower limit of detection reaches 5.13 x 10<-9>mol/L (S/N = 3). The electrode achieves high selectivity, high sensitivity and high anti-interference capability, and is applicable for detection of L-Glu in pig serum; results are consistent with results obtained through high performance liquid chromatography, and the recovery rate ranges from 97.4% to 105.5%; and therefore, the method can provide a new approach for the L-Glu detection, and achieves significant application prospects in the field of life science.

The invention discloses a preparation method of a composite photocatalytic material of porous nano Fe3O4-N doped with Ni/Zn-MOFs/g-C3N4. The method comprises the steps that firstly, urea is calcined to prepare a graphite-phase carbon nitride (g-C3N4) material; then, by adopting ferric chloride, sodium acetate and ethanediamine as raw materials and ethylene glycol as a solvent, a constant-temperature reaction is conducted at 180-220 DEG C for 5-7 h, centrifugal separation is conducted to form solids, and Fe3O4 nano particles with porous structures are obtained; then, zinc nitrate hexahydrate, nickel nitrate hexahydrate, the g-C3N4 material and the Fe3O4 nano particles are added to a mixed solution of N,N-dimethylformamide and ethylene glycol, and a mixed reaction solution is obtained; the mixed reaction solution is transferred to a stainless steel reaction kettle with a polytetrafluoroethylene lining, the temperature is increased to 140-160 DEG C, a constant-temperature reaction is conducted for 5-7 h, centrifugal separation is conducted to form solids, and the composite photocatalytic material of Fe3O4-N doped with Ni/Zn-MOFs/g-C3N4 is obtained. The composite photocatalytic material is of a core-shell structure, g-C3N4 is used as a core, porous nano Fe3O4-N is dispersed on a shell of Ni/Zn-MOFs doped with N, and the material has obvious photocatalytic activity.

The invention discloses a 3,4-dammarane tetracyclic triterpenoid compound with the structural formula shown in formulas (1)-(4). The invention further discloses an extraction method and application ofthe 3,4-dammarane tetracyclic triterpenoid compound. The 3,4-dammarane tetracyclic triterpenoid compoundcan significantly increase the consumption of insulin resistance adipocyte glucose, inhibit generation of IR adipocyte FFA, reduce the content of free fatty acid in cells and activate PPAR<gamma> to a certain extent.

At the aim of the situations of degraded soil fertility and low chemical fertilizer nutrient utilization rates in China, and with the combination of the advantage of rich waste biomass resources in China, the invention discloses a novel process for preparing a carbon-based slow-release composite fertilizer from waste biomass. The novel process specifically comprises the following steps: carrying out hydrothermal treatment on waste biomass under specific conditions, mixing the hydrothermal biomass with a composite fertilizer according to a certain ratio, or carrying out co-hydrothermal treatment on waste biomass and the composite fertilizer under specific conditions, and carrying out drying and pelletization. According to the novel process, the waste biomass is adopted as a raw material, sothat the carbon-based slow-release composite fertilizer prepared by using the process is low in price, and in addition, the carbon-based slow-release composite fertilizer has the advantages of beinghigh in fertilizer efficiency, high in nutrient utilization rate, good in soil repairing function, and the like.

The invention provides a preparation method of a pre-dyed luminescent protein marker. The method comprises the steps of obtaining a protein A gene, a protein G gene, an MBP gene, and a heavy chain constant region gene (CH gene) of mouse and rabbit source antibodies, and cutting or combining the genes according to the sizes of various proteins in the pre-dyed luminescent protein marker; and expressing the proteins, purifying the proteins, and mixing the proteins of different molecular weights at a certain ratio to obtain the luminescent protein marker. The luminescent protein marker is capable of identifying a first antibody or a second antibody of different sources, increasing the binding sites of the proteins and thus strengthening an optical signal. The luminescent protein marker can be exposed and displayed on an X film after being incubated together with the first antibody or the second antibody without an extra antibody; the luminescent protein can be combined with IgG from species, such as human, rat, mouse and rabbit or anti-rabbit, and anti-mouse second antibodies; and compared with luminescent markers of other companies, the pre-dyed luminescent protein marker has a wider indication range and has the molecular weight ranging from 16kDa to 200kDa.

The invention belongs to the technical field of novel functional materials and biosensor detection, and provides a preparation method and application of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles. Particularly, with the graphene loaded with the TaC and the gold-coated decahedral silver nanoparticles as a marker, an electrochemical immunosensor for detecting tumor markers CA199 and CA25 antigens is prepared.