111results about How to "Add binding sites" patented technology

The invention discloses an aluminum-based copper-plated graphene film composite material with high heat-conducting property and a preparation method thereof. The preparation method comprises the following steps of: de-oiling an aluminum substrate sample for 3min in a 65 DEG C de-oiling solution and then cleaning by clear water; carrying out bright dipping on the de-oiled sample for 2min in a 25 DEG C bright dipping solution and then cleaning by clear water; carrying out zinc dipping on the sample (subjected to the bright dipping) for 2min in a 25 DEG C primary zinc dipping solution and then cleaning by clear water; carrying out zinc dipping on the sample (subjected to the primary zinc dipping) for 2min in a 25 DEG C secondary zinc dipping solution and then cleaning by clear water; plating the sample (subjected to the secondary zinc dipping) for 5min by direct current in a 25 DEG C priming copper plating solution, wherein the current density is 1-4A / dm2, the polar distance is 5cm, and then cleaning by clear water; plating the sample (subjected to the copper plating) for 20-40min by directional pulse in a 25 DEG C composite copper plating graphene plating solution and then cleaning by clear water, wherein the current density is 1-4A / dm2 and the polar distance is 5cm. The preparation method has the following advantages that the requirement on equipment is low, the operation is convenient, the process route is clear and the thickness of the film is controllable.

The invention discloses a method for preparing a graphene oxide film and a graphene oxide film product. The method comprises the following steps: mixing graphene oxide dispersion liquid and amino terminal-hyperbranched or dendritic polymer, carrying out suction filtering to prepare the graphene oxide film. The invention further discloses a method for preparing the graphene oxide film for sustained release of drug and a graphene oxide film product, and the method comprises the steps of mixing the graphene oxide dispersion liquid and the amino terminal-hyperbranched or dendritic polymer, adding a medicine with an aromatic nucleus into the mixed solution, and finally carrying out suction filtering. According to the method, the graphene oxide is crosslinked by means of the amino terminal-hyperbranched or dendritic polymer to form a flat lamellar film, so that the film has a relatively high film stability in an aqueous environment, the sustained-release controlled-release performance of drug can be improved, and the drug loading capacity of the film can be improved; and the graphene oxide film for sustained-release of drug is slowly released in a damp or aqueous environment and has the advantages of long maintaining duration and remarkable antibacterial effect.

The invention relates to a preparation method of a molecular imprinting polymer. The preparation method comprises the following steps: dissolving dimethyl methylphosphonate (DMMP), a functional monomer A and a functional monomer B in a pore former, performing ultrasonic degassing for at least 5 minutes, placing the mixture in a refrigerator for above 8 hours to obtain a template molecule-monomer complex, adding a crosslinking agent in the template molecule-monomer complex, performing ultrasonic vibration for at least 30 minutes, introducing nitrogen for at least 15 minutes while adding an initiator, sealing, performing thermal polymerization in a suspension prepared from polyvinyl alcohol, cooling to the room temperature to separate out precipitates, centrifuging, performing Soxhlet extraction on the obtained polymer to ensure that the polymer has no imprinted molecules, washing microspheres with chloroform to remove residual methanol and acetic acid, and drying the microspheres in vacuum to reach a constant weight and obtain DMMP molecular imprinting polymer microspheres. The DMMP molecular imprinting polymer microspheres prepared by the method have the advantages of obviously higher adsorption capacity, larger specific surface area and good selectivity.

The invention discloses a temperature-sensitive hepatic cell culture support material and a preparation method thereof, and relates to an intelligent tissue engineering support material and technology, in particular to a temperature-sensitive hepatic cell culture support material, a method and technology. The support material is characterized in that: the surface of the material is relatively lyophobic at the temperature of 37 DEG C and is suitable for cell adherence / spreading and cell propagation, but the surface is hydrophilic at the temperature of 32 DEG C, so cells are desorbed from the surface automatically. The preparation method of the cell culture support material comprises the following steps of: (1) surface cleaning; (2) the temperature-sensitive carboxylation modification of a TCPS surface; (3) the preparation of amido lactobionic acid (L-NH2); and (4) the saccharifying modification of the TCPS surface. Through the preparation method, the biocompatibility and cell-bound loci of the support material are enhanced, the adsorption rate of the cells on the material surface is increased, and the intelligent support material which is suitable for hepatic tissue engineering is obtained.

The invention relates to a small-caliber artificial blood vessel with the surface grafted with a heparin coating and a preparation method of the small-caliber artificial blood vessel. A small-caliberblood vessel is adopted as a substrate. The preparation method comprises the following steps: soaking the surface of the substrate in a dopamine solution for reaction, coating the surface of the substrate with multiple layers of polydopamine coatings, performing chemical bond combination grafting with polyethyleneimine, and finally activating heparin by utilizing 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) to obtain the heparinized surface through amido bond combination. The invention has the characteristics of small caliber, favorable mechanical properties, hydrophilicity and long-acting anticoagulation; the preparation method is environmentally friendly and efficient in compounding, the prepared heparin coating is uniform, the grafting amount of heparin is higher and can reach 4.9 mu g / cm < 2 >, and good market application prospects are achieved.

The invention provides an indinavir ketone derivative with the alkyl-amino side chain, which is a change for the connecting chain between the benzene ring and tertiary amine nitrogen in the molecule of the phenoxy indinavir ketone compound; the distance between the benzene ring and tertiary amine nitrogen is extended; the connecting chain of the original one-carbon unit (methylene) is changed into a connecting chain with a 2 to 4 carbon atoms, or a connecting chain with the carbonyl, so as to get a series of novel phenoxy indinavir ketone derivative with the alkyl-amino side chain on the benzene ring. The novel synthesized phenoxy indinavir ketone derivative has the strong inhibitory activity for AChE and the weak inhibition for BuChE; the selectivity is high; the compound can be used in the drug for the treatment of the disease of Alzheimer. The method of the invention is reasonable in design, simple in operation and strong in practicality. In the invention, the structure of the indinavir ketone derivative with the alkyl-amino side chain is shown in the formula (see figure).

The invention provides a hollow braided pipe modification method, which comprises: modifying a hollow braided pipe by using a modification solution containing a polyphenol compound and a cross-linkedpolymer. The present invention further provides a hollow fiber film preparation method, which comprises: A, modifying the hollow braided pipe as a support material by using the modification method; B,carrying out mineralization treatment on the hollow braided pipe treated in the step A with a salt solution; and C, carrying out surface coating on the hollow braided pipe treated in the step B witha film forming liquid. According to the present invention, the preparation process is simple, the used raw materials are cheap, and the method is suitable for industrial production.

The invention relates to a method for preparing an in-situ tissue engineering blood vessel by a composite process. According to the invention, a three-layer composite intravascular stent which is similar to a natural vascular structure and is used for in-situ tissue engineering is prepared by combining rapid prototyping, electrostatic spinning and wet spinning processes; wherein the inner layer isa tubular stent provided with an axial groove by utilizing 3D printing and electrostatic spinning technologies; a middle layer is a tubular stent which is prepared by wet spinning and is provided with circumferentially arranged fibers; and the outer layer is the tubular stent which is prepared by the coatable inner layer obtained by electrostatic spinning and the disordered fibers at the middle layer. Through heparinization, the vascular endothelial cell growth factor is loaded, so that cells can be collected in a host, the infection opportunity of in-vitro culture is reduced, the preparationof in-situ tissue engineering blood vessels is realized, and the method has a wide prospect in the field of tissue engineering blood vessels.

The invention discloses an electro-chemical cell sensor with double targeting and visual functions and a preparation method of the electro-chemical cell sensor. According to the electro-chemical cell sensor and the preparation method, a three-dimensional carbon nano tube (3D-MWCNTs) structure is established to increase the effective bonding area with targeting micromolecules, indium-tin-oxide conducting glass (ITO) is selected as an electrode, a novel targeted micromolecule AsC8H10NS2 is synthesized to be taken as a probe on the basis of the characteristic of specificity bonding between trivalent arsenic and adjacent sulfhydryl groups, the probe can carry out target recognition on the adjacent sulfhydryl protein on tumor cell membranes, the electro-chemical cell sensor carries out qualitative and quantitative detection on tumor cells by utilizing an electro-chemical method, the states of the tumor cells are directly observed and captured by a microscope technology, so that the electro-chemical cell sensor with double targeting and visual functions is successfully established.

The invention discloses a preparation method of an edible nanofiber preservative film. The preparation method comprises the following steps: (1) fully dissolving pullulan in ethyl alcohol with the volume fraction of 90%-95% at room temperature to enable the mass concentration of pullulan to be 16%-18%, and after fully dissolving pullulan, adding carboxymethyl cellulose to enable the mass concentration of the mixed solution to be 1%-2%, so as to obtain a basic spinning solution; (2) adding tea polyphenol and sodium citrate into the basic spinning solution to enable the mass concentrations of tea polyphenol and sodium citrate to be 0.5%-1% and 0.3%-0.5% respectively, fully stirring until uniformly dissolved, and leaving to stand until air bubbles completely disappear, so as to obtain a spinning solution; (3) placing the spinning solution on an electrostatic spinning machine for spinning, so as to obtain the edible nanofiber preservative film in the form of non-woven fabric. Compared with the coating method, the preparation method has the advantages that the prepared preservative film is good in the preservation effect, the raw material usage amount is small, and the prepared preservative film is lighter.

The invention discloses a sulfur-nitrogen co-doped graphene loaded nano-silicon three-dimensional electrode material and a preparation method thereof. The electrode material comprises graphene doped with sulfur atoms and nitrogen atoms, graphene is of a complex three-dimensional network structure, and nano-silicon is distributed between graphene sheet layers; the preparation method comprises the following steps: (1) selecting raw materials; (2) mixing; (3) performing hydrothermal synthesis; (4) performing naturally cooling; and (5) removing impurities and drying. The preparation method has thebeneficial effects that the electrode material provided by the invention is good in cycling stability and excellent in conductivity; in the lithium intercalation-deintercalation process, the initialenergy density of the electrode is improved, and the initial cycle coulombic efficiency, the cycle performance and the rate capability are relatively high; the preparation method disclosed by the invention is simple, and meanwhile, the complexity of an electrode plate manufacturing process is reduced due to the design of a three-dimensional structure.

The invention relates to a flowerlike manganese dioxide / carbon composite material and a preparation method and application thereof. The preparation method comprises the steps that S1, a carbon material and an acid solution are mixed, and a dispersion liquid is obtained through dispersion; S2, a potassium permanganate solution is added into the dispersion liquid for a reaction at the temperature of60-95 DEG C under a stirring condition; and S3, after the reaction is over, washing, suction filtering and drying are performed, and then the flowerlike manganese dioxide / carbon composite material isobtained. Through the preparation method, raw materials are easy to obtain, reserves are rich, price is low, the process is simple, high-temperature and high-pressure treatment is not needed, and industrial production is benefited; the prepared flowerlike manganese dioxide / carbon composite material has good conductivity, active substances can quickly and sufficiently react, and stability is good;and since MnO<2> of a layered crystal structure is large in capacity, a formed battery can provide higher specific capacity, the application range of the battery is expanded, and accordingly the competitiveness of the battery is improved.

The invention discloses a method for preparing self-repairing hydrogel by guiding polymerization through a natural polymer template. The method comprises the following steps: taking a linear-chain natural polymer containing aldehyde groups as a template, fixing monomeric molecules containing amino groups on the natural polymer template through the action of Schiff base under alkaline conditions, and then, performing free radical polymerization to prepare polymer hydrogel with high rebound resilience and self-repairing performance. By virtue of existence of the natural linear-chain polymer template in the method disclosed by the invention, polymerization of monomers can be effectively guided, and dynamic chemical cross-linking sites are increased, thereby improving the mechanical strength and the self-repairing capability of the hydrogel. The hydrogel prepared by the method disclosed by the invention has broad application prospects in the related fields of artificial tissues and biomedicine.

The invention relates to a method for improving the enzymolysis saccharifying efficiency of hemp straw. The method is mainly characterized in that before enzymolysis saccharifying of hemp straw, the following pretreatment is performed: (1) crushing hemp straw, performing high-temperature sterilization, then inoculating white rot fungi into the crushed straw, and performing culture for 7-28 days toobtain biologically pre-treated hemp straw; and (2) mixing the biologically pre-treated hemp straw with a low-concentration alkali and hydrogen peroxide mixed solution, and performing treatment for 20-30 h at 40-60 DEG C to obtain pre-treated hemp straw, wherein the mass concentration of alkali in the mixed solution is 0.5-5%, and the volume concentration of hydrogen peroxide is 2.5-3.5%. Beforealkali oxygen treatment of hemp, biological pretreatment is performed at first, so that the consumption and the concentration of alkali in the alkali oxygen treatment process are reduced, the treatment time is shortened, the treatment efficiency is improved, and the pretreatment effect is improved.

The invention discloses a preparation method of a fuel cell membrane electrode CCM. The method comprises the following steps: preparing a hydrophilic catalyst, a perfluorosulfonic acid resin solution and a dispersing agent into a slurry with uniform components in a dispersing manner, and transferring the slurry to two sides of a proton exchange membrane to obtain the fuel cell membrane electrode CCM, wherein the EW value of the proton exchange membrane is 700 to 900; and the EW value of the perfluorinated sulfonic acid resin is 700 to 800; The hydrophilic catalyst and a short-side-chain low-EW-value resin are adopted, binding sites of water are increased so that the affinity of the catalyst layer to water is improved, a rich proton transmission network structure is constructed, the proton conduction rate of the catalyst layer is increased, the proton exchange membrane with a high water conduction capacity is adopted, the diffusion capacity of water generated by a cathode to an anode is enhanced, and a self water balance capability of the MEA under the condition that external humidification is not performed is realized. The whole preparation process does not introduce additional substances, does not need to change a membrane electrode preparation process flow, and is easy to popularize and apply.

The invention discloses epoxy resin conductive adhesive for electronic packaging, and belongs to the technical field of adhesive. The epoxy resin conductive adhesive for electronic packaging is prepared from the following steps: stirring and mixing graphene oxide and water in an amount which is 10 to 20 times of the weight of the graphene oxide, cryogenic grinding, dissolving, filtering and dryingto obtain pretreated graphene oxide; and performing heating, stirring and refluxing reaction on 5 to 6 parts of graphene oxide, 8 to 10 parts of polyamine curing agent and 15 to 20 parts of diluent,adding 50 to 60 parts of epoxy resin, 5 to 6 parts of modified nano-copper powder, 5 to 6 parts of modified silver nanowire and 1 to 2 parts of coupling agent, stirring and mixing to obtain the epoxyresin conductive adhesive for electronic packaging. The epoxy resin conductive adhesive for electronic packaging, which is provided by the technical scheme of the invention, has excellent electrical conductivity and shearing strength.

The invention relates to a method for preparing a fluorescer for showing potential fingerprint through the viscous side of adhesive tap and belongs to the field of fingerprint identification technology. The method comprises the following steps: distilled water, sodium sulfite and selenium powder are added to a first vessel and is deoxidized and is heated and reflows in a water bath to obtain a seleno sodium sulfite aqueous solution; after deionized water is added to a second vessel for deoxidization, cadmium chloride and a sulfhydryl compound are added to the vessel; the PH value of the mixture is regulated; the seleno sodium sulfite aqueous solution is added to the vessel and reflows in boiling water to obtain a cadmium selenide nanocrystal solution; after the deionized water is added to a third vessel for deoxidization, the cadmium chloride and the sulfhydryl compound are added to the vessel; the PH value of the mixture is regulated; and the obtained solution is added to the cadmium selenide nanocrystal solution, is dripped with an equimolar sodium sulfide aqueous solution and reflow in boiling water to obtain the fluorescer. The method has a simple process and available materials; and the fluorescer has perfect showing efficiency, sensitivity, accuracy and repeatability and wide application prospect.

The invention relates to a method for detecting a cancer marker MicroRNA based on a three-dimensional graphene biosensor. The three-dimensional graphene biosensor consists of a glass substrate and a three-dimensional graphene layer. Two sides of the upper surface of the glass substrate are provided with indium tin oxide (ITO), and the upper surface of part of indium tin oxide on both sides are covered with the three-dimensional graphene layer. The part uncovered with the three-dimensional graphene on one side serves as a source electrode, and the part uncovered with the three-dimensional graphene on the other side serves as a drain electrode. The method of the invention requires no labeling, is simple to operate and convenient to use, has higher selectivity and specificity under lower usevoltage, and has good safety.

The invention discloses an L-Glutamic acid (L-Glu) detection method and sensor based on a molecularly imprinted polymer (MIP) membrane modified electrode. The method comprises the following steps: preparation of an MWCNTs dispersion solution, preparation of an electropolymerization base solution, preparation of the MIP membrane modified electrode, L-Glu detection, and the like. Results show that the MIP / MWCNTs / GCE has sensitive L-Glu identification characteristics; and when the L-Glu concentration ranges from 1.00 x 10<-8>mol / L to 1.00 x 10<-5>mol / L, a good linear relationship (R<2> = 0.9923)between peak currents and the concentration is achieved, and the lower limit of detection reaches 5.13 x 10<-9>mol / L (S / N = 3). The electrode achieves high selectivity, high sensitivity and high anti-interference capability, and is applicable for detection of L-Glu in pig serum; results are consistent with results obtained through high performance liquid chromatography, and the recovery rate ranges from 97.4% to 105.5%; and therefore, the method can provide a new approach for the L-Glu detection, and achieves significant application prospects in the field of life science.

The invention provides a heavy metal ion porous adsorbent as well as a preparation method and application thereof. The preparation method of the heavy metal ion porous adsorbent comprises the following steps: (1) dropwise adding a dispersion phase containing an initiator and an electrolyte into a continuous phase containing ethyl glycidyl acrylate, divynyl benzene and an emulsifier, thus obtaining concentrated emulsion; (2) carrying out polymerization reaction on the concentrated emulsion, and washing and drying a polymerization reaction product, thus obtaining a porous polymer; (3) carrying grafting reaction on polyacrylic acid and the porous polymer under the existence of a catalyst, thus obtaining the heavy metal ion porous adsorbent. The heavy metal ion porous adsorbent provided by the invention has good adsorption effect on various heavy metal ions.

The invention discloses a preparation method of a dumbbell-shaped molecular imprinting stirring rod. The method comprises the following steps of: dipping on the dumbbell-shaped stirring rod in alkali and acid in turn for carrying out activation and silanization reaction; further dipping the dumbbell-shaped stirring rod into a trimethylolpropane tricrylic acid ester solution to carry out grafting reaction; putting a template molecule vardenafil and a functional monomer into a pore-foaming agent for prepolymerization, subsequently adding a cross-linking agent and an initiator and putting into the grafted dumbbell-shaped stirring rod to polymerize; repeating the polymerization reaction of the dumbbell-shaped stirring rod, further aging, and finally washing the template molecules so as to obtain the dumbbell-shaped molecular imprinting stirring rod. The stirring rod prepared by using the preparation method is combined with a chromatograph and can be applied to rapid separation and enrichment of nafil compounds in complex matrixes such as food, medicines and health-care products, so that accurate detection on a sample, with high selectivity and high sensitivity, is achieved.

The invention discloses a preparation method of a composite photocatalytic material of porous nano Fe3O4-N doped with Ni / Zn-MOFs / g-C3N4. The method comprises the steps that firstly, urea is calcined to prepare a graphite-phase carbon nitride (g-C3N4) material; then, by adopting ferric chloride, sodium acetate and ethanediamine as raw materials and ethylene glycol as a solvent, a constant-temperature reaction is conducted at 180-220 DEG C for 5-7 h, centrifugal separation is conducted to form solids, and Fe3O4 nano particles with porous structures are obtained; then, zinc nitrate hexahydrate, nickel nitrate hexahydrate, the g-C3N4 material and the Fe3O4 nano particles are added to a mixed solution of N,N-dimethylformamide and ethylene glycol, and a mixed reaction solution is obtained; the mixed reaction solution is transferred to a stainless steel reaction kettle with a polytetrafluoroethylene lining, the temperature is increased to 140-160 DEG C, a constant-temperature reaction is conducted for 5-7 h, centrifugal separation is conducted to form solids, and the composite photocatalytic material of Fe3O4-N doped with Ni / Zn-MOFs / g-C3N4 is obtained. The composite photocatalytic material is of a core-shell structure, g-C3N4 is used as a core, porous nano Fe3O4-N is dispersed on a shell of Ni / Zn-MOFs doped with N, and the material has obvious photocatalytic activity.

The invention provides a preparation method of ionic liquid for selectively separating a water-ethanol azeotropic system. The preparation method is characterized in that cationic groups in the synthetic ionic liquid have hydroxyl. The preparation method comprises the following steps: reacting N-methylimidazole with 2-bromoethanol under the condition of constant-temperature ultrasonic reflux to prepare bromo-1-methyl-3-(2-hydroxylethyl) imidazole; then, purifying bromo-1-methyl-3-(2-hydroxylethyl) imidazole to obtain the ionic liquid of bromo-1-methyl-3-(2-hydroxylethyl) imidazole. The preparation method has the advantages that the synthesis process is simple, and the prepared ionic liquid is non-volatile, good in stability, friendly to the environment and obvious in 'salt effect'; groups in the ionic liquid are capable of selectively bonding component water in the water-ethanol azeotropic system to generate a solvation reaction under the actions of chemical affinity, hydrogen bond force and electrostatic attraction of ions to produce a nonvolatile ionic liquid-water associated complex so that water vapor pressure is lowered, the volatility of water is reduced, steam phase partial pressure and relative volatility of ethanol are increased and the 'salting out' phenomenon appears and thus the water-ethanol azeotropic system is separated.

The invention discloses a 3,4-dammarane tetracyclic triterpenoid compound with the structural formula shown in formulas (1)-(4). The invention further discloses an extraction method and application ofthe 3,4-dammarane tetracyclic triterpenoid compound. The 3,4-dammarane tetracyclic triterpenoid compoundcan significantly increase the consumption of insulin resistance adipocyte glucose, inhibit generation of IR adipocyte FFA, reduce the content of free fatty acid in cells and activate PPAR<gamma> to a certain extent.

At the aim of the situations of degraded soil fertility and low chemical fertilizer nutrient utilization rates in China, and with the combination of the advantage of rich waste biomass resources in China, the invention discloses a novel process for preparing a carbon-based slow-release composite fertilizer from waste biomass. The novel process specifically comprises the following steps: carrying out hydrothermal treatment on waste biomass under specific conditions, mixing the hydrothermal biomass with a composite fertilizer according to a certain ratio, or carrying out co-hydrothermal treatment on waste biomass and the composite fertilizer under specific conditions, and carrying out drying and pelletization. According to the novel process, the waste biomass is adopted as a raw material, sothat the carbon-based slow-release composite fertilizer prepared by using the process is low in price, and in addition, the carbon-based slow-release composite fertilizer has the advantages of beinghigh in fertilizer efficiency, high in nutrient utilization rate, good in soil repairing function, and the like.

The invention discloses a high-stability ceramic honeycomb catalyst and a preparation method thereof. The catalyst comprises a catalyst carrier and an active component, the active component is loadedon the surface of the catalyst carrier, wherein the catalyst carrier comprises silicon carbide, a sintering aid, talcum powder, diatomite, a modified additive and the like; traditional ceramic catalyst carriers are basically processed and formed through molds, the carriers are simple in overall structure and are mostly of straight-through hole ceramic structures, and the catalyst carrier high in strength and large in specific surface area is prepared through the laser sintering technology in the invention, so that the loading rate of active component is increased, and the catalytic activity ofthe finished product is further improved. According to the high-stability ceramic honeycomb catalyst and the preparation method thereof disclosed by the invention, the process operation is simple, the component proportion is proper, the prepared catalyst is excellent in catalytic performance and high in catalytic activity, the strength of the catalyst carrier is high, and the catalyst can be applied to industrial VOCs treatment and has high practicability.

The invention provides a preparation method of a pre-dyed luminescent protein marker. The method comprises the steps of obtaining a protein A gene, a protein G gene, an MBP gene, and a heavy chain constant region gene (CH gene) of mouse and rabbit source antibodies, and cutting or combining the genes according to the sizes of various proteins in the pre-dyed luminescent protein marker; and expressing the proteins, purifying the proteins, and mixing the proteins of different molecular weights at a certain ratio to obtain the luminescent protein marker. The luminescent protein marker is capable of identifying a first antibody or a second antibody of different sources, increasing the binding sites of the proteins and thus strengthening an optical signal. The luminescent protein marker can be exposed and displayed on an X film after being incubated together with the first antibody or the second antibody without an extra antibody; the luminescent protein can be combined with IgG from species, such as human, rat, mouse and rabbit or anti-rabbit, and anti-mouse second antibodies; and compared with luminescent markers of other companies, the pre-dyed luminescent protein marker has a wider indication range and has the molecular weight ranging from 16kDa to 200kDa.

The invention discloses an allyl imine bridged ferrocene-rhodamine B multichannel response receptor molecule and application thereof in the efficient detection of Pd<2+> ions in a water phase and living cells. The allyl imine bridged ferrocene-rhodamine B multichannel response receptor molecule has the advantages that an electric signal response group ferrocene and an optical signal response group rhodamine B are connected through an allyl imine bridge to form the receptor molecule, binding sites are increased, the receptor molecule has multichannel response functions such as electrochemistry, ultraviolet, fluorescence and color comparison, the Pd<2+> ions in the water phase and living cells can be selectively, efficiently and fast detected by methods such as cyclic voltammetry, an raviolet visible light method and a fluorescence spectrum method, lowest detection limit is 8.46*10<-9>M, and the receptor molecule is promising in application prospect.

The invention belongs to the technical field of novel functional materials and biosensor detection, and provides a preparation method and application of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles. Particularly, with the graphene loaded with the TaC and the gold-coated decahedral silver nanoparticles as a marker, an electrochemical immunosensor for detecting tumor markers CA199 and CA25 antigens is prepared.

The invention is a melanodermia virus quickly-detecting reagent box, including: a detecting device; tween-phosphate buffer liquid containing ox serum albumin; tween-phosphate buffer liquid; low osmotic liquid; gold labeled anti-melanodermia virus monoclonal antibody probe, prepared of two colloidal gold labeled monoantibodies E and D in proportion; the E and D are anti-melanodermia virus bursa membrane antibody E and anti-melanodermia virus nucleus coat antibody D secreted by two hybrid tumor cells, respectively; the conservation numbers of the two hybrid tumor cells are CCTCC-C200421 and CCTCC-C200422, respectively. As the E and D are used as detecting antibodies, they can obviously improve detecting sensitivity. The detecting reaction only takes 3 minutes without hatching and any instrument and equipment, and their detecting sensitivity is the same as that of spot immune printing method. The reagent box has the characters: simple and convenient, quick, accurate, etc.