Preparation method and application of immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles
A technology of silver nanoparticles and immunosensors, which is applied in the field of immunoassay and biosensing, can solve the problems of poor stability of silver nanoparticles, achieve superior dispersion, good conductivity, and improve sensitivity
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Embodiment 1
[0042] Embodiment 1 A preparation method of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles, the steps are as follows:
[0043] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;
[0044] (2) Place the above electrodes in HAuCl with a mass fraction of 0.8% 4 In the solution, scan at -0.2V for 30s to form an electrodeposited gold base on the electrode surface, rinse with ultrapure water, and dry;
[0045] (3) Continue to drop 6 µL, 10 µg / mL tumor marker capture antibody onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;
[0046] (4) Continue to add 3 µL, 1.0 mg / mL bovine serum albumin solution dropwise to the surface of the electrode, rinse the surface of the electrode with ultrapure water, and dry it in a refrigerator at 4 °C;
[0047] (5) Add 6 μL, 0.0005 ~ 40 ng / mL of a series of tumor m...
Embodiment 2
[0049] Embodiment 2 A preparation method of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles, the steps are as follows:
[0050] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;
[0051] (2) Place the above electrodes in HAuCl with a mass fraction of 0.9% 4 In the solution, scan at -0.2V for 30s to form an electrodeposited gold base on the electrode surface, rinse with ultrapure water, and dry;
[0052] (3) Continue to drop 6 µL, 12 µg / mL tumor marker capture antibody onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;
[0053] (4) Continue to add 3 µL, 2.0 mg / mL bovine serum albumin solution dropwise to the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;
[0054] (5) Add 6 μL, 0.0005 ~ 40 ng / mL of a series of tumor marker antigen ...
Embodiment 3
[0056] Embodiment 3 A preparation method of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles, the steps are as follows:
[0057] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;
[0058] (2) Place the above electrodes in HAuCl with a mass fraction of 1.0% 4 In the solution, scan at -0.2V for 30s to form an electrodeposited gold base on the electrode surface, rinse with ultrapure water, and dry;
[0059] (3) Continue to drop 6 µL, 15 µg / mL tumor marker capture antibody onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;
[0060] (4) Continue to add 3 µL, 3.0 mg / mL bovine serum albumin solution dropwise to the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;
[0061] (5) Add 6 μL, 0.0005 ~ 40 ng / mL of a series of tumor marker antigen ...
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