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Preparation method and application of immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles

A technology of silver nanoparticles and immunosensors, which is applied in the field of immunoassay and biosensing, can solve the problems of poor stability of silver nanoparticles, achieve superior dispersion, good conductivity, and improve sensitivity

Inactive Publication Date: 2017-06-06
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Decahedral silver nanoparticles (Ag DeNps) have more catalytic active sites than silver nanospheres, but the stability of silver nanoparticles is relatively poor. Uniformly coated gold nanoparticles can effectively improve the performance of silver nanoparticles. particle stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 A preparation method of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles, the steps are as follows:

[0043] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0044] (2) Place the above electrodes in HAuCl with a mass fraction of 0.8% 4 In the solution, scan at -0.2V for 30s to form an electrodeposited gold base on the electrode surface, rinse with ultrapure water, and dry;

[0045] (3) Continue to drop 6 µL, 10 µg / mL tumor marker capture antibody onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0046] (4) Continue to add 3 µL, 1.0 mg / mL bovine serum albumin solution dropwise to the surface of the electrode, rinse the surface of the electrode with ultrapure water, and dry it in a refrigerator at 4 °C;

[0047] (5) Add 6 μL, 0.0005 ~ 40 ng / mL of a series of tumor m...

Embodiment 2

[0049] Embodiment 2 A preparation method of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles, the steps are as follows:

[0050] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0051] (2) Place the above electrodes in HAuCl with a mass fraction of 0.9% 4 In the solution, scan at -0.2V for 30s to form an electrodeposited gold base on the electrode surface, rinse with ultrapure water, and dry;

[0052] (3) Continue to drop 6 µL, 12 µg / mL tumor marker capture antibody onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0053] (4) Continue to add 3 µL, 2.0 mg / mL bovine serum albumin solution dropwise to the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0054] (5) Add 6 μL, 0.0005 ~ 40 ng / mL of a series of tumor marker antigen ...

Embodiment 3

[0056] Embodiment 3 A preparation method of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles, the steps are as follows:

[0057] (1) A glassy carbon electrode with a diameter of 3 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0058] (2) Place the above electrodes in HAuCl with a mass fraction of 1.0% 4 In the solution, scan at -0.2V for 30s to form an electrodeposited gold base on the electrode surface, rinse with ultrapure water, and dry;

[0059] (3) Continue to drop 6 µL, 15 µg / mL tumor marker capture antibody onto the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0060] (4) Continue to add 3 µL, 3.0 mg / mL bovine serum albumin solution dropwise to the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0061] (5) Add 6 μL, 0.0005 ~ 40 ng / mL of a series of tumor marker antigen ...

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Abstract

The invention belongs to the technical field of novel functional materials and biosensor detection, and provides a preparation method and application of an immunosensor based on graphene loaded with TaC and gold-coated decahedral silver nanoparticles. Particularly, with the graphene loaded with the TaC and the gold-coated decahedral silver nanoparticles as a marker, an electrochemical immunosensor for detecting tumor markers CA199 and CA25 antigens is prepared.

Description

technical field [0001] The invention belongs to the technical field of immune analysis and biosensing, and provides a preparation method and application of an immunosensor loaded with TaC and graphene coated with gold-coated decahedral silver nanoparticles to realize signal amplification. Background technique [0002] Sensitive detection of tumor markers, clinical early detection of tumors, screening of high-risk groups of tumors, differential diagnosis of benign and malignant tumors, judgment of tumor development, observation and evaluation of tumor treatment effects, and prediction of tumor recurrence and prognosis Prediction has a great impact and attracts a lot of people's attention. [0003] Electrochemical immunosensors have been widely used in the detection of tumor markers. Sandwich-type electrochemical immunosensors combine high-specificity immunoassay technology and high-sensitivity electrochemical analysis technology. They have high sensitivity, simple preparation...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 李月云张晓波高增强吕慧冯金慧苏晓楠刘丰硕董云会
Owner SHANDONG UNIV OF TECH
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