Preparation method of dimethyl methylphosphonate (DMMP) molecular imprinting polymer microspheres

A technology of dimethyl methylphosphonate and molecular imprinting, which is applied in the field of bioengineering, can solve the problem of undiscovered reports on imprinted polymers of dimethyl methylphosphonate, limited binding sites between template molecules and functional monomers, and difficult Trace detection of template molecules and other issues, to achieve the effect of increasing specific surface area, increasing binding sites, and improving accuracy

Inactive Publication Date: 2012-07-25
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the current use and selection of functional monomers have relatively large limitations and blindness, resulting in limited binding sites between template molecules and functional monomers in molecular imprinting, and the prepared molecularly imprinted polymers have very low adsorption capacity during use. Low, difficult to implement trace detection of template molecules
[0005] A search of the existing technical literature found that no relevant reports related to the topical dimethyl methylphosphonate (DMMP) imprinted polymer of the present invention have been found so far

Method used

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  • Preparation method of dimethyl methylphosphonate (DMMP) molecular imprinting polymer microspheres
  • Preparation method of dimethyl methylphosphonate (DMMP) molecular imprinting polymer microspheres
  • Preparation method of dimethyl methylphosphonate (DMMP) molecular imprinting polymer microspheres

Examples

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Effect test

Embodiment 1

[0027] Take 1mmol of dimethyl methylphosphonate (DMMP) and 4mmol of methacrylic acid (MAA), dissolve them in 20ml of chloroform solution, vibrate ultrasonically for 10min, and put them in the refrigerator for 8 hours to form template molecule-monomer complexes. Weigh 20 mmol of cross-linking agent ethylene glycol dimethacrylate (EGDMA) and add it to the prepared template molecule-monomer complex, ultrasonically degas for 30 min, and pass through at least N 2 After 15 minutes, add 0.3 mmol of initiator azobisisobutyronitrile (AIBN), seal it, and polymerize in water at 60°C for 24 hours, take it out and cool it to room temperature, centrifuge, and use methanol / acetic acid 9 / 1 (V / V) to obtain the polymer Wash until there are no imprinted molecules, then wash with chloroform, dry in a vacuum oven at 50°C to constant weight, and finally obtain a molecularly imprinted polymer with an average particle size of 20-50 μm. The inventive effect is as follows: figure 1 As shown, the surfac...

Embodiment 2

[0031] Take 1mmol of dimethyl methylphosphonate (DMMP), 4mmol of acrylic acid (MAA), and 1mmol of zinc porphyrin, dissolve them in 20ml of chloroform solution, oscillate ultrasonically for 5min, and put them in the refrigerator for 8 hours to form a template molecule-unit body complex, weighed 20mmol cross-linking agent trimethylolpropane trimethacrylate (TRIM) and added it to the prepared template molecule-monomer complex, ultrasonic degassed for 30min, and at least N 2 After 15 minutes, add 0.4 mmol of initiator azobisisobutyronitrile (AIBN), seal, and polymerize in water at 60°C for 28 hours, take it out and cool to room temperature, centrifuge, and the obtained polymer is mixed with methanol / acetic acid 9 / 1 (V / V) Wash until there are no imprinted molecules, then wash with chloroform, dry in a vacuum oven at 75°C to constant weight, and finally obtain a molecularly imprinted polymer with an average particle size of 20-50 μm. The inventive effect is as follows: figure 2 As ...

Embodiment 3

[0034]Take 1mmol of dimethyl methylphosphonate (DMMP), 4mmol of methacrylate (MMA), and 0.3mmol of gadolinium porphyrin, dissolve them in 10ml of chloroform solution, oscillate ultrasonically for 5min, and put them in the refrigerator for 8 hours to form Template molecule-monomer complex, weigh 20mmol cross-linking agent ethylene glycol dimethacrylate (EGDMA) and add it to the prepared template molecule-monomer complex, ultrasonic degassing for 30min, at least N 2 After 15 minutes, add 0.28 mmol of initiator azobisisobutyronitrile (AIBN), seal it, and polymerize it in water at 70°C for 24 hours, take it out and cool it to room temperature, and centrifuge. The obtained polymer is mixed with methanol / acetic acid 9 / 1 (V / V) Wash until there are no imprinted molecules, then wash with chloroform, dry in a vacuum oven at 70°C to constant weight, and finally obtain a molecularly imprinted polymer with an average particle size of 20-50 μm. The inventive effect is as follows: image 3 A...

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Abstract

The invention relates to a preparation method of a molecular imprinting polymer. The preparation method comprises the following steps: dissolving dimethyl methylphosphonate (DMMP), a functional monomer A and a functional monomer B in a pore former, performing ultrasonic degassing for at least 5 minutes, placing the mixture in a refrigerator for above 8 hours to obtain a template molecule-monomer complex, adding a crosslinking agent in the template molecule-monomer complex, performing ultrasonic vibration for at least 30 minutes, introducing nitrogen for at least 15 minutes while adding an initiator, sealing, performing thermal polymerization in a suspension prepared from polyvinyl alcohol, cooling to the room temperature to separate out precipitates, centrifuging, performing Soxhlet extraction on the obtained polymer to ensure that the polymer has no imprinted molecules, washing microspheres with chloroform to remove residual methanol and acetic acid, and drying the microspheres in vacuum to reach a constant weight and obtain DMMP molecular imprinting polymer microspheres. The DMMP molecular imprinting polymer microspheres prepared by the method have the advantages of obviously higher adsorption capacity, larger specific surface area and good selectivity.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing molecularly imprinted polymers. Background technique [0002] Organophosphorus pesticides are currently the most widely used chemical pesticides. They are highly lethal and difficult to protect, and have always been a research hotspot in the field of chemical detection. The organophosphorus pesticides remaining in the environment form phosphorylated cholinesterase with cholinesterase in the organism, which inhibits the activity of cholinesterase, leading to neurological disorders and even death. Dimethyl methyl phosphonate (DMMP) is a simulant of organophosphorus pesticides. It has the characteristics of similar molecular structure to organophosphorus pesticides, low toxicity, and safe operation. -P=O, -P-O-bonds, in-depth research on them can provide reference and basis for establishing an effective method for detecting organophosphorus poisons. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/14C08F212/36C08F222/38C08F220/06C08F220/04C08F220/14C08F226/06C08F220/56C08F230/04C08J9/26B01J20/26B01J20/28B01J20/30
Inventor 法焕宝齐卉尹伟侯长军霍丹群罗小刚
Owner CHONGQING UNIV
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