L-Glutamic acid (L-Glu) detection method and sensor based on molecularly imprinted polymer (MIP) membrane modified electrode

A technology for modifying electrodes and molecular imprinting, which is applied in the field of chemical/biological sensing to achieve the effects of increasing sensitivity, increasing electronic conductivity, and superior detection limit

Pending Publication Date: 2019-09-13
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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  • Application Information

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Problems solved by technology

Currently, glutamic acid (L-Glu) molecularly imprinted sensor (MIP / MWCNTs / GCE) method has not been reported

Method used

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  • L-Glutamic acid (L-Glu) detection method and sensor based on molecularly imprinted polymer (MIP) membrane modified electrode
  • L-Glutamic acid (L-Glu) detection method and sensor based on molecularly imprinted polymer (MIP) membrane modified electrode
  • L-Glutamic acid (L-Glu) detection method and sensor based on molecularly imprinted polymer (MIP) membrane modified electrode

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Embodiment Construction

[0030] All the reagents used in the examples are of analytical grade (AR), and the experimental water is all ultrapure water (resistivity ≥ 18.3 MΩ·cm). In the following description, the descriptions of amino acids all use English abbreviations.

[0031] 1. Experimental process

[0032] 1. Preparation of MWCNTs dispersion

[0033] 1.0mg of MWCNTs was placed in 1.0mL of N-N dimethylformamide solution and sonicated for 1h to obtain MWCNTs dispersion (1.0mg / mL).

[0034] 2. Preparation of electropolymerization base solution

[0035] Accurately weigh 0.5606g 4,6-diaminoresorcinol (monomer) and 0.1471g L-glutamic acid (template), dissolve with PBS buffer solution of pH = 5.0 and set the volume to 100mL to prepare 4,6 -Diaminoresorcinol (4.0mM) and L-glutamic acid (1.0mM) mixed liquid, that is, the electropolymerization base solution with a monomer and template molar concentration ratio of 4:1.

[0036] 3. Preparation of Molecularly Imprinted Polymer Membrane Modified Electrode ...

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Abstract

The invention discloses an L-Glutamic acid (L-Glu) detection method and sensor based on a molecularly imprinted polymer (MIP) membrane modified electrode. The method comprises the following steps: preparation of an MWCNTs dispersion solution, preparation of an electropolymerization base solution, preparation of the MIP membrane modified electrode, L-Glu detection, and the like. Results show that the MIP/MWCNTs/GCE has sensitive L-Glu identification characteristics; and when the L-Glu concentration ranges from 1.00 x 10<-8>mol/L to 1.00 x 10<-5>mol/L, a good linear relationship (R<2> = 0.9923)between peak currents and the concentration is achieved, and the lower limit of detection reaches 5.13 x 10<-9>mol/L (S/N = 3). The electrode achieves high selectivity, high sensitivity and high anti-interference capability, and is applicable for detection of L-Glu in pig serum; results are consistent with results obtained through high performance liquid chromatography, and the recovery rate ranges from 97.4% to 105.5%; and therefore, the method can provide a new approach for the L-Glu detection, and achieves significant application prospects in the field of life science.

Description

technical field [0001] The invention belongs to the technical field of chemical / biological sensing, and in particular relates to a method for detecting L-glutamic acid based on a molecularly imprinted polymer film modified electrode and a sensor. Background technique [0002] As an acidic non-essential amino acid, L-glutamic acid (L-Glu) has an important impact on the metabolism and control of organisms, the diagnosis and treatment of diseases, and the development of medicine and food industries. L-Glu is an important excitatory neurotransmitter in organisms, which plays a vital role in brain functions such as learning and memory. For most eukaryotes and mammals, L-Glu can also be used as a nitrogen source to provide energy. In addition, L-Glu is also widely used in the food industry as a flavor enhancer. The normal L-Glu content in the human body should not exceed 30mg / kg. Excessive L-Glu intake can lead to certain neurological diseases such as chorea and Parkinson's synd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/333G01N27/48B82Y5/00B82Y15/00
CPCB82Y5/00B82Y15/00G01N27/308G01N27/3277G01N27/3278G01N27/3335G01N27/48
Inventor 曹忠唐飞何军意段薇袁璇肖忠良
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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