Electro-chemical cell sensor with double targeting and visual functions and preparation method thereof

A cell sensor and electrochemical technology, applied in the field of electrochemistry, can solve the problems of large scale, high cost, and time-consuming, etc., and achieve the effect of increasing the binding site, good conductivity, and high transparency

Inactive Publication Date: 2014-09-10
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for early detection of cancer mainly include X-ray diagnosis, enzyme-linked immunoassay (PCR), immunohistochemistry, flow cytometry, etc. These methods have high sensitivity and accuracy, but are costly and expensive. Factors such as more time and large scale of instruments limit their wide application

Method used

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  • Electro-chemical cell sensor with double targeting and visual functions and preparation method thereof
  • Electro-chemical cell sensor with double targeting and visual functions and preparation method thereof
  • Electro-chemical cell sensor with double targeting and visual functions and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of sensor

[0023] 1. Functionalization of multi-walled carbon nanotubes: First, ultrasonically ultrasonicate the multi-walled carbon nanotubes in a 3:1 mixture of sulfuric acid and nitric acid for 16 hours, then filter and wash them with deionized water until the filtrate is neutral, and vacuum conditions Tumble dry.

[0024] 2. ITO glass surface pretreatment: First, put the ITO glass into twice distilled water, acetone, isopropanol, ethanol, and twice distilled water for 15 minutes, and then prepare ammonia water: hydrogen peroxide (NH3 ·H 2 O:H 2 o 2 =1:1) boil in the solution for 30min;

[0025] 3. Amination of ITO electrode surface: quickly add 10mM p-phenylenediamine solution to 1M NaNO under ice bath conditions 2 In the solution, react in the dark for 3 minutes to synthesize a diazonium cation solution, pour it into the electrolytic cell, and the detection solution is prepared from a 10mM pH7.4 PBS solution containing [Fe(CN 6 )...

Embodiment 2

[0028] Example 2: Electrochemical Behavior and Atomic Force Microscopy (AFM) Characterization of the Sensor Construction Process

[0029] During the electrode modification process, the dual qualitative methods of electrochemical cyclic voltammetry and electrochemical impedance were used for characterization. First, the treated bare ITO electrode is placed in the detection cell, and as the surface of the electrode is gradually modified with p-phenylenediamine (AP), MWCNTs, small molecule A S C 8 h 10 NS 2 , in 10 mM pH 7.4 PBS containing 5 mM K 3 Fe(CN) 6 and 0.1M KCl solution (sweep rate: 100mV s -1 ) for cyclic voltammetry detection, the current value gradually decreased; in 10mM pH7.4PBS containing [Fe(CN 6 )] 3- / [Fe(CN 6 )] 4- (10mM, 1:1) and 0.1M KCl solution for electrochemical impedance (0.1-10 5 Hz) detection, the corresponding electron transfer resistance R et Gradually increased; combined with the AFM spectrum ( figure 1 ), the surface height of ITO gradu...

Embodiment 3

[0030] Example 3: Targeted Molecular Probe A S C 8 h 10 NS 2 Study on Specificity of ITO Sensors Modified by Labeled Three-dimensional Carbon Nanotubes

[0031] 1. The bare ITO electrode, AP-ITO electrode, 3D-MWCNTs / ITO electrode, A S C 8 h 10 NS 2 The 3D-MWCNTs / ITO electrodes were soaked in 1mL HL-60 cell suspension respectively, and placed in a cell culture incubator (37°C, 5% CO 2 Humidified atmosphere) for 3 hours of incubation, PBS was used to wash away non-specifically bound cells, and the electrode was placed in the detection cell for electrochemical impedance detection. Bare ITO electrodes, AP / ITO electrodes have basically no electrochemical response, 3D-MWCNTs / ITO electrodes have weak electrochemical response signals, MWCNTs have a small amount of non-specific adsorption to cells, A S C 8 h 10 NS 2 Electron transfer resistance R of 3D-MWCNTs / ITO electrode et significantly increased, indicating that the A S C 8 h 10 NS 2 Small molecule probes have the a...

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Abstract

The invention discloses an electro-chemical cell sensor with double targeting and visual functions and a preparation method of the electro-chemical cell sensor. According to the electro-chemical cell sensor and the preparation method, a three-dimensional carbon nano tube (3D-MWCNTs) structure is established to increase the effective bonding area with targeting micromolecules, indium-tin-oxide conducting glass (ITO) is selected as an electrode, a novel targeted micromolecule AsC8H10NS2 is synthesized to be taken as a probe on the basis of the characteristic of specificity bonding between trivalent arsenic and adjacent sulfhydryl groups, the probe can carry out target recognition on the adjacent sulfhydryl protein on tumor cell membranes, the electro-chemical cell sensor carries out qualitative and quantitative detection on tumor cells by utilizing an electro-chemical method, the states of the tumor cells are directly observed and captured by a microscope technology, so that the electro-chemical cell sensor with double targeting and visual functions is successfully established.

Description

technical field [0001] The invention relates to the field of electrochemistry, in particular to an electrochemical cell sensor with dual functions of targeting and visualization and a preparation method thereof. Background technique [0002] Cancer is one of the major diseases that threaten human health, and cancer metastasis is also an important cause of death of patients. Early detection and early treatment not only improves the survival rate of patients, but also improves the quality of life of patients. Rapid and sensitive detection of tumor cells is an important guarantee for grasping the best time for treatment and formulating the best treatment plan. Early diagnosis reduces the rate of cancer metastasis, thereby reducing the mental and economic burden of patients. At present, the methods for early detection of cancer mainly include X-ray diagnosis, enzyme-linked immunoassay (PCR), immunohistochemistry, flow cytometry, etc. These methods have high sensitivity and accu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 贾能勤徐亚楠黄楚森
Owner SHANGHAI NORMAL UNIVERSITY
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