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Small-caliber artificial blood vessel with surface grafted with heparin coating and preparation method thereof

A technology of artificial blood vessel and heparin coating, applied in coating, pharmaceutical formulation, anticoagulation treatment, etc., can solve the problems of surface hydrophobicity of small diameter artificial blood vessel, low efficiency of heparin coupling and grafting, and lack of anticoagulant properties. , to achieve good market application prospects, improve anticoagulant properties, and the effect of simple and fast preparation methods

Inactive Publication Date: 2020-08-18
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a small-diameter artificial blood vessel grafted with a heparin coating on the surface and its preparation method, which overcomes the low active group content of the artificial blood vessel polymer material and the heparin coupling and grafting problem in the prior art. Low efficiency, and the defect that active substances such as electrostatic adsorption of biological macromolecules are easy to fall off, and will not damage the mechanical properties of the material itself, and the reaction conditions are mild
Put the artificial blood vessel in the dopamine solution to undergo the self-polymerization process of dopamine to obtain a polydopamine coating, then place it in a polyethyleneimine solution at 60°C to react grafted polyethyleneimine, and finally add it to the heparin solution of EDC / NHS to react Finally, small-diameter polyurethane blood vessels, polyester artificial blood vessels and polytetrafluoroethylene artificial blood vessels grafted with heparin coating on the surface were prepared, which solved the problem that the surface of small-diameter artificial blood vessels was hydrophobic and did not have anticoagulant properties

Method used

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  • Small-caliber artificial blood vessel with surface grafted with heparin coating and preparation method thereof
  • Small-caliber artificial blood vessel with surface grafted with heparin coating and preparation method thereof
  • Small-caliber artificial blood vessel with surface grafted with heparin coating and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] (1) preparing a small-caliber polyurethane artificial blood vessel with an internal diameter of 4 mm by solvent coagulation method, the preparation method is: utilizing tetrahydrofuran to dissolve the polyurethane particle material ( AL polycarbonate-based ether-free polyurethane, purchased from Canada HnG Medical Technology Co., Ltd.), prepares PU solution, and the mass concentration of polyurethane in the solution is 15% or with DMF as solvent will be purchased from the liquid polyurethane of Canada HnG Medical Technology Co., Ltd. AR (solid content 22%) was diluted to a polyurethane mass concentration of 15%. Dip-coat the polyurethane solution on a glass shaft with a diameter of 4 mm, and then put the glass shaft into water. After 24 hours, remove the glass shaft, wash it repeatedly with absolute ethanol and deionized water, and then dry it to obtain a small diameter by solvent solidification method. Polyurethane vascular prosthesis.

[0046] (2) Take the small-di...

Embodiment 2

[0057] (1) Prepare an electrospun small-diameter polyurethane artificial blood vessel with an inner diameter of 4 mm, the preparation method of which is: utilize DMF to dissolve the polyurethane particle material ( AL polycarbonate-based ether-free polyurethane was purchased from Canada HnG Medical Technology Co., Ltd.), and the spinning solution was prepared, and the mass concentration of polyurethane in the spinning solution was 10%. Or use DMF as a solvent to purchase liquid polyurethane from Canada HnG Medical Technology Co., Ltd. AR (solid content 22%) was diluted to a polyurethane mass concentration of 10%. The electrospinning voltage was 16kV, the flow rate was 1.5mL / h, and the spinning time was 24h. The filaments were collected by a rod-shaped drum collector with a diameter of 4mm. After spinning, it was thoroughly washed with deionized water, and after drying, an electrospun small-diameter polyurethane artificial blood vessel was obtained.

[0058] (2) Using the s...

Embodiment 3

[0067] (1) Prepare an electrospun small-diameter polyurethane artificial blood vessel with an inner diameter of 3 mm. The preparation method is: using a mixed solvent of tetrahydrofuran and dimethylformamide (DMF) to dissolve polyurethane particle materials (Carbothane TM , Tecoflex TM , or Tecothane TM Polymers in thermoplastic polyurethane (TPU) products were purchased from Lubrizol (Lubrizol) Co., Ltd., USA. Carbothane TM Is an aliphatic, aromatic, polycarbonate-based thermoplastic polyurethane. Tecoflex TM It is an aliphatic polyether based thermoplastic polyurethane. Thermoplastic polyurethane medical grade polymers are aromatic polyether and polyester products. Tecothane TMThere are two types of thermoplastic polyurethanes: aromatic polyether-based products and polyester products. ) to prepare spinning solution, the volume ratio of tetrahydrofuran and DMF in the spinning solution is 1:1, and the mass concentration of polyurethane is 20%. The electrospinning vo...

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Abstract

The invention relates to a small-caliber artificial blood vessel with the surface grafted with a heparin coating and a preparation method of the small-caliber artificial blood vessel. A small-caliberblood vessel is adopted as a substrate. The preparation method comprises the following steps: soaking the surface of the substrate in a dopamine solution for reaction, coating the surface of the substrate with multiple layers of polydopamine coatings, performing chemical bond combination grafting with polyethyleneimine, and finally activating heparin by utilizing 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) to obtain the heparinized surface through amido bond combination. The invention has the characteristics of small caliber, favorable mechanical properties, hydrophilicity and long-acting anticoagulation; the preparation method is environmentally friendly and efficient in compounding, the prepared heparin coating is uniform, the grafting amount of heparin is higher and can reach 4.9 mu g / cm < 2 >, and good market application prospects are achieved.

Description

technical field [0001] The invention belongs to the field of artificial blood vessels and their preparation, in particular to a small-caliber artificial blood vessel with a surface grafted with a heparin coating and a preparation method thereof. Background technique [0002] Cardiovascular disease is a common disease that endangers human life and health. Atherosclerosis and other cardiovascular diseases cause high morbidity and mortality worldwide every year. When a blood vessel cannot maintain its normal function due to atherosclerosis, damage or aging of the artery, a blood vessel transplantation is required to replace it. [0003] The development and research of artificial blood vessels have solved the problem of blood vessel transplantation to a certain extent. In the field of medium and large-caliber vascular transplantation, polyester (PET) blood vessels and expanded polytetrafluoroethylene (ePTFE) blood vessels have good antithrombotic ability and good long-term pate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/34A61L27/50A61L27/54A61L33/00A61L33/08
CPCA61L27/18A61L27/34A61L27/507A61L27/54A61L33/0011A61L33/0088A61L33/08A61L2400/18C08L5/10C08L79/04C08L75/04
Inventor 洪枫胡高铨李格丽陈琳欧阳晨曦李佳荣王趁红
Owner DONGHUA UNIV
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