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Method for preparing in-situ tissue engineering blood vessel by composite process

A tissue engineering and composite technology technology, applied in the field of biomedical tissue engineering, to achieve the effects of simple and feasible removal steps, ensuring mechanical properties, and preventing blood vessel rupture

Active Publication Date: 2019-11-29
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the material selection and preparation process of tissue engineering blood vessels still need to be developed and improved.

Method used

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  • Method for preparing in-situ tissue engineering blood vessel by composite process
  • Method for preparing in-situ tissue engineering blood vessel by composite process
  • Method for preparing in-situ tissue engineering blood vessel by composite process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Such as Figure 5 As shown, a method for preparing in situ tissue engineered blood vessels by a composite process comprises the following steps:

[0037] a. 3D printing water-soluble materials to prepare stent collection rods: the structure is a cylindrical hollow structure, such as figure 1 As shown: the inner diameter is 3.5mm, the outer diameter is 4.5mm, and the outer surface of the cylinder is designed with 8 ridges. The size of the raised structure is 50mm in length, 0.4mm in width, and 0.2mm in height. The collection rod is made of polyvinyl alcohol, a water-soluble polymer material Shaped by FDM printing. The nozzle temperature of the printer is 160°C, the bed temperature is 60°C, the nozzle moving speed is 20mm / s, and the layer thickness is set to 0.15mm.

[0038] b. The inner layer of tissue engineering blood vessels was prepared by electrospinning process, such as figure 2 Shown: To increase the number of end groups available for biomolecular attachment, ...

Embodiment 2

[0045] This embodiment is basically the same as Embodiment 1, and the special features are:

[0046] In this embodiment, a method for preparing in situ tissue engineered blood vessels by a composite process is to prepare ridges of different widths and numbers on the outside of the stent collection rod to cause the inner layer of the stent to be patterned, including the following steps:

[0047] a. 3D printing water-soluble materials to prepare vascular stent collection rods: design vascular stent collection rods: its structure is a cylindrical hollow structure with an inner diameter of 4mm and an outer diameter of 5mm, and 6 ridges are designed on the outer surface of the cylinder, and the size of the raised structure is long 50mm, 0.5mm wide, and 0.3mm high, the collection rod is made of water-soluble polymer material polyvinyl alcohol and formed by FDM printing. The nozzle temperature of the printer is 160°C, the bed temperature is 60°C, the nozzle moving speed is 20mm / s, an...

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Abstract

The invention relates to a method for preparing an in-situ tissue engineering blood vessel by a composite process. According to the invention, a three-layer composite intravascular stent which is similar to a natural vascular structure and is used for in-situ tissue engineering is prepared by combining rapid prototyping, electrostatic spinning and wet spinning processes; wherein the inner layer isa tubular stent provided with an axial groove by utilizing 3D printing and electrostatic spinning technologies; a middle layer is a tubular stent which is prepared by wet spinning and is provided with circumferentially arranged fibers; and the outer layer is the tubular stent which is prepared by the coatable inner layer obtained by electrostatic spinning and the disordered fibers at the middle layer. Through heparinization, the vascular endothelial cell growth factor is loaded, so that cells can be collected in a host, the infection opportunity of in-vitro culture is reduced, the preparationof in-situ tissue engineering blood vessels is realized, and the method has a wide prospect in the field of tissue engineering blood vessels.

Description

technical field [0001] The invention relates to the technical field of biomedical tissue engineering, in particular to a method for preparing blood vessels for tissue engineering in situ by a composite process. Background technique [0002] With the acceleration of population aging, many diseases related to human blood vessels, such as atherosclerosis and coronary heart disease, are on the rise. When the human body's own blood vessels cannot normally supply blood to the human body due to factors such as disease and trauma, surgical operations such as repair, intravascular stents, or autologous vein grafting should be used to treat the damaged blood vessels. Generally, the saphenous vein is considered to be a good substitute for autologous blood vessels in small-diameter vascular reconstruction, but some patients cannot use autologous veins due to their own diseases or other reasons, so using tissue engineered blood vessels as a substitute for tissue vascular repair has becom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/34A61L27/50A61L27/54
CPCA61L27/18A61L27/34A61L27/507A61L27/54A61L27/50C08L67/04
Inventor 陈箫姚远刘随红张亦弛
Owner SHANGHAI UNIV
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