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Method for improving enzymolysis saccharifying efficiency of hemp straw

A technology for saccharification efficiency and hemp, which is applied in the field of enzymatic hydrolysis of hemp stalks promoted by the combined use of microorganisms and alkaline hydrogen peroxide, can solve the problems of low efficiency of direct enzyme treatment, shorten pretreatment time, improve swelling effect, and increase yield The effect of the amount of reducing sugar

Inactive Publication Date: 2018-03-23
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the resistance of the surface of natural fibers to biological enzymes, the direct enzymatic treatment efficiency is low. In order to achieve high enzymatic hydrolysis efficiency, certain pretreatment must be carried out to increase the combination of the surface and enzymes.

Method used

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  • Method for improving enzymolysis saccharifying efficiency of hemp straw
  • Method for improving enzymolysis saccharifying efficiency of hemp straw

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Experimental program
Comparison scheme
Effect test

Embodiment 1～3

[0048] This embodiment relates to a method for enzymatic hydrolysis of hemp straw, which mainly includes the following steps:

[0049] A. Pretreatment of hemp straw

[0050] 1) Crush hemp straw (ramie, hemp or kenaf) to 200 mesh, adjust the water content to 70% after high-temperature sterilization, mix according to the volume-to-mass ratio of the bacterial solution to the hemp straw at 1:10, and inoculate it for activation After cultivating the Pleurotus eryngii bacteria liquid for 21 days at 28° C., the cultured mixture was extracted at normal temperature with a citric acid-sodium citrate buffer solution with a pH of 4.8, filtered, and the filter residue was dried to a constant weight. Obtain hemp straw after biological pretreatment;

[0051] 2) Mix the biologically pretreated hemp straw with a mixed solution of sodium hydroxide and hydrogen peroxide at a mass volume ratio of 1:20, treat at 40°C for 24 hours, filter, wash the filter residue with distilled water until neutral...

Embodiment 4

[0056] Using ramie straw as a raw material, compared with Example 1, the difference is that in step 1), the straw is treated with Phanerochaete chrysosporium. After testing, the reducing sugar content of ramie straw was 159 mg / g.

Embodiment 5

[0058] Using ramie stalks as raw materials, compared with Example 1, the difference is that in step 1), the stalks are treated with Pleurotus ostreatus. After testing, the reducing sugar content of the ramie straw was 376 mg / g.

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Abstract

The invention relates to a method for improving the enzymolysis saccharifying efficiency of hemp straw. The method is mainly characterized in that before enzymolysis saccharifying of hemp straw, the following pretreatment is performed: (1) crushing hemp straw, performing high-temperature sterilization, then inoculating white rot fungi into the crushed straw, and performing culture for 7-28 days toobtain biologically pre-treated hemp straw; and (2) mixing the biologically pre-treated hemp straw with a low-concentration alkali and hydrogen peroxide mixed solution, and performing treatment for 20-30 h at 40-60 DEG C to obtain pre-treated hemp straw, wherein the mass concentration of alkali in the mixed solution is 0.5-5%, and the volume concentration of hydrogen peroxide is 2.5-3.5%. Beforealkali oxygen treatment of hemp, biological pretreatment is performed at first, so that the consumption and the concentration of alkali in the alkali oxygen treatment process are reduced, the treatment time is shortened, the treatment efficiency is improved, and the pretreatment effect is improved.

Description

technical field [0001] The invention relates to the field of enzymatic hydrolysis and saccharification of hemp stalks, in particular to a method for promoting the enzymatic hydrolysis of hemp stalks by combining microorganisms and alkaline hydrogen peroxide. Background technique [0002] Hemp crops are important economic crops, including hemp, kenaf, ramie, and flax. They generally have strong resistance to stress, and have the characteristics of rapid growth and high yield. The traditional use of hemp is mainly used as textile raw materials, mainly to harvest fibers, resulting in the underutilization of hemp stalks and hemp leaves accounting for 80% of the biomass. , generally use the method of incineration, which not only causes waste of resources, but also causes environmental pollution when incinerated; hemp straw is rich in cellulose, hemicellulose and a small amount of lignin, which is a rich carbohydrate resource and is used as raw material for production It can ferm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12R1/645
CPCC12P19/00C12P2201/00
Inventor 谢纯良彭源德龚文兵朱作华严理胡镇修
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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