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Biological-chemical combined treatment process for improving saccharifying effect of lignocellulose

A technology of lignocellulose and chemical combination, applied in the field of pretreatment of bioethanol from lignocellulose, can solve the problems of increased difficulty, long time, mild treatment conditions, etc., and achieve low equipment investment, increased economic benefits, and high treatment efficiency The effect of mild conditions

Active Publication Date: 2015-12-23
湖南中森环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical methods, such as acid method and alkali method, in which the effect of acid pretreatment on lignin removal is not obvious, and it is easy to produce microbial fermentation inhibitors, which increases the difficulty of subsequent treatment. In addition, the corrosiveness of acid to pretreatment devices also restricts its One of the important reasons for industrialization; the cost of alkali pretreatment is lower than that of the acid reactor, and the operation is safe, but the recovery and treatment of wastewater and residues are still required
The biological method mainly uses fungi such as white rot for pretreatment. The treatment conditions are mild, but the pretreatment effect is not good, and the time is too long, so it is not easy to put into industrial production

Method used

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  • Biological-chemical combined treatment process for improving saccharifying effect of lignocellulose
  • Biological-chemical combined treatment process for improving saccharifying effect of lignocellulose
  • Biological-chemical combined treatment process for improving saccharifying effect of lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) sieve the rice straw with 40 mesh after pulverizing, wash it twice with ultrapure water, and dry it at 55°C to constant weight;

[0027] (2) Inoculate the LD-1 cells stored on the LB slant in the LB liquid medium, and cultivate at 30°C for 18 hours to obtain the seed liquid of LD-1;

[0028] (3) wherein said LB liquid culture medium each component proportion is: peptone 10g, yeast powder 5g, sodium chloride 10g, distilled water 1L, the LB slant is the agar that adds 15g / L on the basis of above-mentioned formula;

[0029] (4) centrifuge the LD-1 seed liquid obtained in the previous step at 8000 rpm for 5 minutes, discard the supernatant, and collect the cells;

[0030] (5) further the collected LD-1 cells were inoculated in lignocellulose liquid culture medium according to 10% (volume ratio) inoculation amount, and cultivated for 4 days at 30° C. temperature, natural pH;

[0031] (6) wherein the ratio of each component of the lignocellulose liquid culture medium is:...

Embodiment 2

[0037] (1) sieve the rice straw with 40 mesh after pulverizing, wash it twice with ultrapure water, and dry it at 55°C to constant weight;

[0038] (2) Inoculate the LD-1 cells stored on the LB slant in the LB liquid medium, and cultivate at 30°C for 18 hours to obtain the seed liquid of LD-1;

[0039] (3) wherein said LB liquid culture medium each component proportion is: peptone 10g, yeast powder 5g, sodium chloride 10g, distilled water 1L, the LB slant is the agar that adds 15g / L on the basis of above-mentioned formula;

[0040] (4) centrifuge the LD-1 seed liquid obtained in the previous step at 8000 rpm for 5 minutes, discard the supernatant, and collect the cells;

[0041](5) further the collected LD-1 cells were inoculated in lignocellulose liquid culture medium according to 10% (volume ratio) inoculation amount, and cultivated for 4 days at 30° C. temperature, natural pH;

[0042] (6) wherein the ratio of each component of the lignocellulose liquid culture medium is: ...

Embodiment 3

[0048] (1) sieve the rice straw with 40 mesh after pulverizing, wash it twice with ultrapure water, and dry it at 55°C to constant weight;

[0049] (2) Inoculate the LD-1 cells stored on the LB slant in the LB liquid medium, and cultivate at 30°C for 18 hours to obtain the seed liquid of LD-1;

[0050] (3) wherein said LB liquid culture medium each component proportion is: peptone 10g, yeast powder 5g, sodium chloride 10g, distilled water 1L; Described LB slant is the agar that adds 15g / l on the basis of above-mentioned formula;

[0051] (4) centrifuge the LD-1 seed liquid obtained in the previous step at 8000 rpm for 5 minutes, discard the supernatant, and collect the cells;

[0052] (5) further the collected LD-1 cells were inoculated in lignocellulose liquid culture medium according to 10% (volume ratio) inoculation amount, and cultivated for 4 days at 30° C. temperature, natural pH;

[0053] (6) wherein the ratio of each component of the lignocellulose liquid culture medi...

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Abstract

The invention discloses a biological-chemical combined treatment process for improving saccharifying effect of lignocellulose. According to the process, a lignocellulose raw material is subjected to biological treatment by virtue of an efficient lignocelluloses degrading bacterium sphingobacterium sp. LD-1 (preservation number is CGMCC No.10920), so as to remove partial lignin and hemicellulose components in the lignocellulose raw material with the action of microorganisms, and to damage the stable structure of the lignocellulose to a certain degree; and furthermore, the lignocellulose undergoes chemical treatment at low temperature by virtue of alkali / urea, so that the lignocellulose is more loose in structure. The lignocellulose, which is preprocessed by the process disclosed by the invention, achieves significant change in component content and obvious increase in proportion of cellulose; in addition, the structure of the lignocellulose is significantly changed as well, and the surface of the raw material becomes rough, loose and porous, so as to greatly increase an accessible surface during enzymolysis saccharifying. The combined pretreatment process disclosed by the invention has the advantages of being not high in requirement on equipment, simple in operation, mild in processing condition, low in cost and the like, and the saccharifying efficiency of the lignocellulose is greatly improved.

Description

technical field [0001] The invention relates to the technical field of bioethanol pretreatment prepared from lignocellulose, in particular to a combined process of bacteria Sphingobacterium sp.LD-1 and alkali / urea pretreatment of lignocellulose. Background technique [0002] With the intensification of the global energy crisis, the current research on the development of alternative energy sources has become a hot issue. The production of fuel ethanol from lignocellulose has attracted much attention due to its high environmental and economic value. The first generation of biomass fuel ethanol uses sugar and starch as raw materials, but faces the problems of rising costs and competition for land; using agricultural and forestry waste as the second generation biomass raw material to produce fuel ethanol has become the most suitable alternative one of the energy sources. [0003] Lignocellulose is the main product of plant photosynthesis, and its main components are cellulose,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/01
CPCY02E50/10
Inventor 陈跃辉司梦莹戴友芝张年磊周沫
Owner 湖南中森环境科技有限公司
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