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A method of using lignin-degrading bacteria to strengthen sodium carbonate pretreatment of waste biomass

A technology of lignin degrading bacteria and waste biomass, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of improving enzymatic hydrolysis and saccharification efficiency, less secondary pollution, and shorter processing time

Active Publication Date: 2021-01-01
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems existing in the existing waste biomass sodium carbonate pretreatment technology, the present invention provides a method of using lignin-degrading bacteria to strengthen the waste biomass sodium carbonate pretreatment method

Method used

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  • A method of using lignin-degrading bacteria to strengthen sodium carbonate pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen sodium carbonate pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen sodium carbonate pretreatment of waste biomass

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0029] (2) Put the waste biomass in a container of appropriate size, and add Na at a concentration of 8% according to the solid-to-liquid ratio of 1:10 (g / ml). 2 CO 3 The solution was left to stand in a constant temperature environment of 80° C. for 3 hours, and then filtered to obtain a wet residue A.

[0030] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0031] (4) Inoculate the Cupriavidus basilensis B-8 cells stored on the LB slope into LB liquid medium, and culture at 30°C for 18 hours (the optical density at 600nm reaches 0.8-1.0) to obtain the seeds of Cupriavidus basilensis B-8 Liquid; wherein said LB liquid culture medium each composition ratio is: peptone 10g, yea...

Embodiment 2

[0038] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0039] (2) Put the waste biomass in a container of appropriate size, and add Na at a concentration of 6% according to the solid-to-liquid ratio of 1:10 (g / ml). 2 CO 3 The solution was left to stand in a constant temperature environment of 80° C. for 3 hours, and then filtered to obtain wet residue A.

[0040] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0041] (4) Inoculate the Cupriavidus basilensis B-8 cells stored on the LB slope into LB liquid medium, and culture at 30°C for 18 hours (the optical density at 600nm reaches 0.8-1.0) to obtain the seeds of Cupriavidus basilensis B-8 Liquid; wherein said LB liquid culture medium each composition ratio is: peptone 10g, yeast...

Embodiment 3

[0048] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0049] (2) Put the waste biomass in a container of appropriate size, and add Na at a concentration of 4% according to the solid-liquid ratio of 1:10 (g / ml). 2 CO 3 The solution was left to stand in a constant temperature environment of 80° C. for 3 hours, and then filtered to obtain a wet residue A.

[0050] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0051] (4) Inoculate the Cupriavidus basilensis B-8 cells stored on the LB slope into LB liquid medium, and culture at 30°C for 18 hours (the optical density at 600nm reaches 0.8-1.0) to obtain the seeds of Cupriavidus basilensis B-8 Liquid; wherein said LB liquid culture medium each composition ratio is: peptone 10g, yeast ...

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Abstract

The invention discloses a method for reinforcing the waste biomass sodium carbonate pretreatment by utilizing lignin-degrading bacteria. According to the method, based on waste biomass sodium carbonate pretreatment, residual lignin and hemicellulose in waste biomass are further removed by utilizing the lignin-degrading bacterium Cupriavidus basilensis B-8 having a preservation number of CGMCC No.4240 and improving the culture condition, steady straight rod state of waste biomass is loosened, and the surface of the waste biomass shows a porous flake shape, so that the accessible surface during enzymolysis saccharification is greatly improved. The method can be used for improving the enzymolysis rate of sodium carbonate sodium pretreatment by nearly 40 percent with remarkable effect, and has the advantages of being short in treatment time, simple in operation, small in secondary pollution, low in cost and the like.

Description

Technical field: [0001] The invention belongs to the technical field of biomass new energy, and in particular relates to a method for using lignin-degrading bacteria to strengthen sodium carbonate pretreatment of waste biomass. Background technique: [0002] In recent years, in order to reduce the dependence on fossil fuels, governments and scientific research institutions of various countries have vigorously carried out the development and research of renewable energy. Lignocellulose in waste biomass is mainly composed of cellulose, hemicellulose and lignin, which contains a huge amount of carbon resources. It has become the second generation of biomass energy and is currently a green product such as bioethanol, bioplastics, and biodiesel. The most important carbon source for biological fermentation. The hemicellulose and lignin in lignocellulose are tightly embedded around the cellulose, and without any treatment, the efficiency of saccharification and fermentation is ext...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12R1/01
CPCC12P19/14C12P2201/00
Inventor 石岩柴立元颜旭张可菁司梦莹杨志辉
Owner CENT SOUTH UNIV
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