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Pretreatment method for improving enzymatic hydrolysis and saccharification efficiency of non-wood fiber raw material

A technology of non-wood fiber and saccharification efficiency, which is applied in the field of pretreatment to improve the efficiency of enzymatic hydrolysis and saccharification of non-wood fiber raw materials, and can solve the problem of low utilization rate

Inactive Publication Date: 2012-06-27
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a large agricultural country, with a huge annual output of crop straw (about 700 million tons, equivalent to 500 million tons of standard coal), but the utilization rate is very low

Method used

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  • Pretreatment method for improving enzymatic hydrolysis and saccharification efficiency of non-wood fiber raw material
  • Pretreatment method for improving enzymatic hydrolysis and saccharification efficiency of non-wood fiber raw material
  • Pretreatment method for improving enzymatic hydrolysis and saccharification efficiency of non-wood fiber raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: Pretreatment and enzymatic hydrolysis method using rice straw as raw material.

[0051] raw material:

[0052] The straw used in the experiment was produced in northern Jiangsu and provided by a paper mill in Jiangsu. After the raw materials are air-dried, the roots are removed, the stems, leaves, and sheaths are kept, and they are cut into 3-5cm grass pieces, mixed evenly, and the water is balanced for later use.

[0053] Cellulase (CCN03110, enzyme activity 118 FPU / mL), xylanase (CFN00244, enzyme activity 153FXU / mL), β-cellobiase (DCN00207, enzyme activity 480 CBU / mL) were provided by Novozymes. Use acetic acid-sodium acetate buffer (pH 4.8) to make cellulase, xylanase and β-cellobiase into a mixed enzyme solution with an enzyme activity ratio of 1FPU: 1.2FXU: 1CBU, and the enzyme activity of the mixed enzyme solution The enzyme activity of the cellulase filter paper was 20 FPU / mL, and excess β-cellobiase was added to prevent the accumulation of cellob...

Embodiment 2

[0064] Embodiment 2: Pretreatment and enzymatic hydrolysis method using wheat straw as raw material.

[0065] raw material:

[0066] The raw material of the experiment is wheat straw. After air-drying, the roots and ears are removed, and the stems, leaves, and sheaths are kept. They are cut into 3-5cm grass pieces, mixed evenly, and the water is balanced for later use.

[0067] The enzyme used in the experiment is a mixture of cellulase (NS50013, enzyme activity 84 FPU / mL), xylanase (NS50014, enzyme activity 109FXU / mL) and β-cellobiase (NC188, enzyme activity 350CBU / mL) , provided by Novozymes. Use acetic acid-sodium acetate buffer (pH 4.8) to mix cellulase, xylanase and β-cellobiase with an enzyme activity ratio of 1FPU: 1.2FXU: 1CBU to make a mixed enzyme solution, and the enzyme in the mixed enzyme solution The enzyme activity of cellulase filter paper is 20FPU / mL.

[0068] Sodium carbonate pretreatment process:

[0069] Sodium carbonate pretreatment is carried out in s...

Embodiment 3

[0086] Embodiment 3: Pretreatment and enzymatic hydrolysis method using wheatgrass leaves and sheaths as raw materials.

[0087] raw material:

[0088] The experimental raw materials are wheat grass leaves and sheaths, which are cut into 3-5 cm grass pieces, mixed evenly, and balanced in water for later use.

[0089] The enzyme used in the experiment is the same as in Example 2.

[0090] Sodium carbonate pretreatment and enzymatic hydrolysis process are identical with embodiment 2.

[0091] Wheatgrass leaves and leaf sheaths are the main components of waste residues in paper mills. At present, there is no better treatment and utilization measures except burning. The main chemical components of wheatgrass leaves and sheaths are shown in Table 2.

[0092] Table 2 Analysis of main chemical components of each part of wheat straw, %

[0093]

[0094]

[0095] Figure 13 It is the content of lignin (Klason lignin, acid-soluble lignin), ash and various polysaccharides (dex...

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Abstract

The invention discloses a pretreatment method for improving enzymatic hydrolysis and saccharification efficiency of a non-wood fiber raw material. The method comprises the following steps of: mixing an aqueous solution of sodium carbonate and the non-wood fiber raw material, pre-impregnating for 30 to 120 minutes at the temperature of between 30 and 90 DEG C, heating to the temperature of between 100 and 180 DEG C, performing heat preservation reaction for 0 to 120 minutes, washing the non-wood fiber raw material by using water, defibering the raw material into fibrous pulp by using a stuff grinder, and thus completing the pretreatment process. The yield of the pretreated non-wood fiber raw material is between 50 and 90 percent, the removal rate of lignin is between 10 and 80 percent, the loss of high glycan is between 0 and 25 percent, a little silicon dioxide is dissolved from the non-wood fiber raw material in the pretreatment solution, and most silicon dioxide is preserved in the pretreated non-wood fiber raw material.

Description

technical field [0001] The invention relates to a pretreatment method for improving the enzymatic hydrolysis and saccharification efficiency of non-wood fiber raw materials. Background technique [0002] Energy and environmental issues have always been major issues restricting the civilization and development of countries all over the world. The reserves of fossil fuels are limited and gradually depleted, and the use of traditional fossil fuels produces a large amount of greenhouse gas CO 2 and other harmful gases, causing serious harm to the environment, and causing economic losses of hundreds of billions of dollars every year caused by catastrophic climate problems around the world. Therefore, looking for low-cost, low-pollution, renewable green energy has become a key research direction for countries all over the world. Since the 1970s, bioenergy has been recognized as one of the most promising renewable energy sources due to its non-polluting, renewable, and non-greenh...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 金永灿黄婷杨林峰李忠正
Owner NANJING FORESTRY UNIV
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