High-capacity double-systematic-circulation cellulose enzymolysis reactor and its application

A technology of cellulase and reactor, which is applied in the direction of enzyme production/bioreactor, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., which can solve the problems of short time consumption, low enzyme consumption, Difficulty in the enzymatic hydrolysis process, etc.

Active Publication Date: 2013-01-16
SHANGHAI ZHONGWEI BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the crystallinity, polymerization degree and tendency of hemp bast fiber are much higher than those of other biomass (such as starch), the enzymatic hydrolysis process with glucose as the conversion target (or other monomers) is quite difficult
At present, there is no satisfactory, efficient, short time-consuming, low energy consumption, and less enzyme consumption equipment and process for enzymolysis and saccharification of hemp fiber

Method used

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  • High-capacity double-systematic-circulation cellulose enzymolysis reactor and its application
  • High-capacity double-systematic-circulation cellulose enzymolysis reactor and its application
  • High-capacity double-systematic-circulation cellulose enzymolysis reactor and its application

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Experimental program
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Effect test

Embodiment 1

[0125] enzymatic saccharification

[0126] use figure 1 The enzymatic saccharification device shown performs enzymatic saccharification according to the following steps:

[0127] 1. Steps:

[0128] 1.1 The slurry solution after pretreatment is prepared into a 100L solution with a substrate concentration of 6%.

[0129] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution, and adjust the pH value to 4.8.

[0130] 1.3 Heat the jacket of the two-body cycle enzymolysis reactor to 50°C;

[0131] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. Wherein the total consumption of cellulase (including endoglucanase, exoglucanase and β-glucosidase) is 1000IU / ml. The slurry solution is poured into the first reaction tank at a speed of 600rpm, and from the first reaction tank into the second reaction tank at a speed of 50rp...

Embodiment 2

[0141] use figure 1 The enzymatic saccharification device shown performs enzymatic saccharification according to the following steps:

[0142] 1. Steps:

[0143] 1.1 The slurry solution after pretreatment is prepared into a 100L solution with a substrate concentration of 6%.

[0144] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution, and adjust the pH value to 4.8.

[0145] 1.3 Heat the jacket of the two-body cycle enzymolysis reactor to 50°C;

[0146] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. Wherein the total consumption of cellulase (including endoglucanase, exoglucanase and β-glucosidase) is 900IU / ml. The slurry solution is poured into the first reaction tank with a rotating speed of 1500rpm, and from the first reaction tank into the second reaction tank with a rotating speed of 120rpm. All the ma...

Embodiment 3

[0156]use figure 1 The enzymatic saccharification device shown performs enzymatic saccharification according to the following steps:

[0157] 1. Steps:

[0158] 1.1 The slurry solution after pretreatment is prepared into a 100L solution with a substrate concentration of 6%.

[0159] 1.2 Dissolve an appropriate amount of citric acid and trisodium citrate in the solution, and adjust the pH value to 4.8.

[0160] 1.3 Heat the jacket of the two-body cycle enzymolysis reactor to 50°C;

[0161] 1.4 Add 75% of the total amount of cellulase (enzyme can be liquid enzyme and / or solid enzyme) into the solution, and position the reaction start time at 0 o'clock at this time. Wherein cellulase is acid cellulase, and the total consumption is 1200IU / ml. The slurry solution is poured into the first reaction tank at a speed of 2800rpm, from the first reaction tank into the second reaction tank at a speed of 250rpm. All the materials carry out two-body circulation between the first reactio...

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Abstract

The invention provides a high-capacity double-systematic-circulation cellulose enzymolysis reactor and its application. The high-capacity double-systematic-circulation cellulose enzymolysis reactor concretely comprises: a first reaction tank (a) comprising a tank body, shearing homogenizers positioned in the tank body, an inlet used for adding a feed material, and an outlet used for discharging the material; a second reaction tank (b) comprising above two series or parallel reaction tubes; a pipeline (c) connecting the first reaction tank with the second reaction tank; and a pipeline (d) connecting all tubes in the second reaction tank, wherein the number of the shearing homogenizers is two or more; and the volume of the first reaction tank is 3-150t, and preferably 30-100t. The enzymolysis reactor has the advantages of high conversion rate, high concentration, short conversion time, low enzyme preparation consumption, low energy consumption and the like when the enzymolysis saccharification of celluloses comprising fibrilia and the like is carried out by the enzymolysis reactor, so the enzymolysis reactor is especially suitable for high-capacity cellulose enzymolysis reaction systems.

Description

technical field [0001] The invention relates to the field of new energy, more specifically, to the field of enzymatic saccharification using plant cellulose as raw material. The invention provides a high-capacity, high-capacity, low-energy-consumption dual-body circulation cellulose enzymolysis reactor and an application thereof, which uses hemp crop fiber as a raw material. Background technique [0002] At present, human beings have a great demand for energy, but resources such as petroleum are non-renewable resources, so people vigorously develop various new energy sources. [0003] Ethanol can be burned alone, and can also be used as a good gasoline oxygenate and high-octane blending component. It has been recognized as one of the most promising renewable clean energy sources. Its production and application have shown a high-speed development trend in the world. The fuel ethanol industry has become an important policy means and economic lever for governments to solve the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12M1/02C12P19/14
Inventor 张士楚朱震黎王恒新乐嘉发黄华明张玟籍
Owner SHANGHAI ZHONGWEI BIOCHEM
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