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Method for enhancing enzymolysis and saccharification efficiency of Chinese silvergrass by using alkaline oxydol

A technology of saccharification efficiency and hydrogen peroxide, which is applied in the directions of biofuel and fermentation, can solve the problems of immature cellulosic fuel ethanol production technology, etc., and achieve the effects of increasing the amount of reducing sugar produced, increasing the swelling effect, and promoting the efficiency of enzymatic hydrolysis.

Inactive Publication Date: 2014-09-03
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Large-scale industrial cellulosic fuel ethanol production technology is not yet mature, and key technologies such as pretreatment and five-carbon sugar fermentation still need to be further explored to improve efficiency and reduce costs, so as to meet production needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Put 5g through 400KGy 60 The Miscanthus powder irradiated by Co-γ rays was put into a triangular flask, and 1% sodium hydroxide solution and 1% hydrogen peroxide were added, mixed and placed in a water bath at 30°C for 5 hours, and then washed with dilute sulfuric acid until neutral. properties, and filtered to obtain the filter residue, put the filter residue in an oven at 60°C and dry for 24 hours to obtain Miscanthus residue; put the Miscanthus residue into 30ml of pH 4.8 buffer solution with 300U cellulase, and then place Shake in a constant temperature oscillator at 50°C with a rotation speed of 150rpm for 96 hours, then take it out and put it in a boiling water bath for 5 minutes to inactivate the enzyme and prevent the enzymolysis reaction from proceeding. After the solution is cooled, filter it, collect the filtrate, and measure the The content of reducing sugar is 498.81mg / g.

Embodiment 2

[0024] Put 5g through 400KGy 60 Co-γ-ray irradiated Miscanthus powder was put into a conical flask, adding 1.2% sodium hydroxide solution and 2% hydrogen peroxide, after mixing, put it in a water bath at 30°C for 5 hours, and then washed it with dilute sulfuric acid until neutral. properties, and filtered to obtain the filter residue, put the filter residue in an oven at 60°C and dry for 24 hours to obtain Miscanthus residue; put the Miscanthus residue into 30ml of pH 4.8 buffer solution with 300U cellulase, and then place Shake in a constant temperature oscillator at 50°C with a rotation speed of 150rpm for 96 hours, then take it out and put it in a boiling water bath for 5 minutes to inactivate the enzyme and prevent the enzymolysis reaction from proceeding. After the solution is cooled, filter it, collect the filtrate, and measure the The content of reducing sugar is 505.08mg / g.

Embodiment 3

[0026] Put 5g through 400KGy 60 Co-γ-ray irradiated Miscanthus powder was put into a conical flask, adding 1.5% sodium hydroxide solution and 3% hydrogen peroxide, after mixing, put it in a water bath at 30°C for 5 hours, and then washed it with dilute sulfuric acid until neutral. properties, and filtered to obtain the filter residue, put the filter residue in an oven at 60°C and dry for 24 hours to obtain Miscanthus residue; put the Miscanthus residue into 30ml of pH 4.8 buffer solution with 300U cellulase, and then place Shake in a constant temperature oscillator at 50°C with a rotation speed of 150rpm for 96 hours, then take it out and put it in a boiling water bath for 5 minutes to inactivate the enzyme and prevent the enzymolysis reaction from proceeding. After the solution is cooled, filter it, collect the filtrate, and measure the The content of reducing sugar is 500.01mg / g.

[0027] Miscanthus changed the compact structure between lignin, cellulose and hemicellulose t...

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Abstract

The invention relates to a method for enhancing enzymolysis and saccharification efficiency of Chinese silvergrass by using alkaline oxydol, which comprises the following steps: irradiating Chinese silvergrass powder with 400KGy60Co-gamma rays; putting the irradiated Chinese silvergrass powder into a sodium hydroxide-oxydol solution with certain concentration, and treating in a water bath at certain temperature for some time; washing the mixed solution subjected to the water bath with dilute sulfuric acid to a neutral state, and filtering to obtain a filtration residue; putting the filtration residue in a drying oven, and drying for some time to obtain the Chinese silvergrass residue; putting the Chinese silvergrass residue into a cellulase-containing buffer solution, and oscillating in a constant-temperature oscillator for some time; treating the oscillated solution in a boiling water bath for some time, cooling, and filtering to obtain a filtrate; and determining the content of reducing sugar in the filtrate. The reducing sugar generated by treating the Chinese silvergrass with cellulase needs to be pretreated before fermentation to produce vehicle fuel ethanol, and a low-dose irradiation / dilute alkali composite technique is utilized for treatment can greatly enhance the enzymolysis and saccharification efficiency of Chinese silvergrass.

Description

technical field [0001] The invention relates to the field of Miscanthus enzymolysis and saccharification, in particular to a method for promoting the efficiency of Miscanthus enzymolysis and saccharification by using alkaline hydrogen peroxide. Background technique [0002] Miscanthus is a perennial grass belonging to the genus C. 4 photosynthetic grasses. Its plant height can reach up to 4m, cross-pollination, self-incompatibility, life span is usually 18-20a, and the longest can reach 25a under suitable conditions. Miscanthus plants are native to East Asia and are widely distributed in tropical, subtropical and temperate regions from Southeast Asia to islands near the Pacific Ocean. The genus includes 17 species in 4 groups, of which 6 species are produced in China, mainly distributed in the vast areas south of the Yangtze River. area. Miscanthus has strong adaptability and can grow in marginal wastelands that lack water and fertilizers as well as in various metal minin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P7/10
CPCY02E50/16Y02E50/10
Inventor 苏小军谭兴和蔡柳彭姿
Owner HUNAN AGRICULTURAL UNIV
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