A method of using lignin-degrading bacteria to strengthen the hydrothermal pretreatment of waste biomass

A technology for lignin degrading bacteria and waste biomass, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to improve carbon source utilization and resource yield, increase accessible surface, and improve enzymatic hydrolysis efficiency Effect

Active Publication Date: 2020-11-17
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problems existing in the existing waste biomass pretreatment technology, the present invention provides a method of using lignin-degrading bacteria to strengthen the hydrothermal pretreatment of waste biomass

Method used

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  • A method of using lignin-degrading bacteria to strengthen the hydrothermal pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the hydrothermal pretreatment of waste biomass
  • A method of using lignin-degrading bacteria to strengthen the hydrothermal pretreatment of waste biomass

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Embodiment 1

[0024] (1) The rice straw was crushed and sieved with 60 meshes, washed twice with ultrapure water, and dried at 60°C until constant weight.

[0025] (2) Put the lignocellulose in a container of appropriate size, add ultrapure water according to the solid-liquid ratio of 1:10 (g / ml), and place it in a constant temperature environment of 160°C, 180°C, and 200°C for 30 minutes. , and the wet residue A was obtained by filtration and separation.

[0026] (3) Rinse the wet residue A obtained by filtering and separating with distilled water repeatedly until the pH of the washing liquid is neutral, and dry at 60° C. to a constant weight to obtain dry residue B.

[0027] (4) Inoculate the Cupriavidus basilensis B-8 bacterium stored in the refrigerator at -80°C in LB liquid medium, and culture it at 30°C for 18 hours (the optical density at 600nm reaches 0.8-1.0) to obtain Cupriavidus basilensis B- 8 seed liquid; wherein the LB liquid culture medium components ratio is: peptone 10g, y...

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Abstract

The invention discloses a method for enhancing waste biomass hydro-thermal pretreatment by using lignin degrading bacteria, and concreatly relates to a method that based on pretreatment of waste biomass by a hydrothermal method, the lignin degrading bacteria (Cupriavidus basilensis B-8, a preservation number is CGMCC No.4240) is used, the culture condition is improved, and the residual lignin in the waste biomass is removed, and the accessible surfaces while enzymatic hydrolysis and saccharification can be greatly increased. The enzymatic hydrolysis efficiency for hydrothermal method pretreatment is increased by about 56%, and the method has the advantages of short processing time, simple operation, no secondary pollution, and low cost.

Description

Technical field: [0001] The invention belongs to the technical field of biomass new energy, and in particular relates to a method for using lignin-degrading bacteria to strengthen hydrothermal pretreatment of waste biomass. Background technique: [0002] In recent years, in order to reduce the dependence on fossil fuels, governments and scientific research institutions of various countries have vigorously carried out the development and research of renewable energy. Lignocellulose in waste biomass is mainly composed of cellulose, hemicellulose and lignin, which contains a huge amount of carbon resources. It has become the second generation of biomass energy and is currently a green product such as bioethanol, bioplastics, and biodiesel. The most important carbon source for biological fermentation. Cellulose in lignocellulose is a long-chain molecule formed by D-glucose through β-1,4 glycosidic bonds, and the long-chain molecules further form a supramolecular stable structur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12R1/01
CPCC12P19/14C12P2201/00
Inventor 石岩柴立元颜旭刘丹卓胜男杨志辉
Owner CENT SOUTH UNIV
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