Method for producing fermentable sugar by red ramie bark fibre enzymolysis

A technology of kenaf hemp skin and kenaf fiber, which is used in fermentation, biofuel and other directions

Active Publication Date: 2009-03-04
SHANGHAI ZHONGWEI BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method converts cellulose to sugars with a saccharification rate of only about 58%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] 1. Preparation: Cut 20kg of kenaf husks into 0.5-1cm long and 1-1.5cm wide pieces for later use.

[0089] 2. Physical degumming: After boiling in a boiling water bath for 30 minutes, rinse the degummed kenaf husk fibers with tap water to remove the degummed products adhered to the fibers, and repeat three times.

[0090] 3. Steam explosion: Steam explosion conditions: 3MPa, 90s, dry and ready for use.

[0091] 4. Pulverization: pulverize to 80 mesh with a plant pulverizer.

[0092] 5. Acid cellulase hydrolysis and saccharification: Completely saccharify cellulose, the substrate concentration is 3%, the acid cellulase activity is 750IU / ml, the pH value is 4.8, the enzyme-catalyzed reaction temperature is 55°C, and the reaction time is 6.0h, thus obtaining Reducing sugars obtained from the degradation of cellulose.

[0093] After the above process, the sugar conversion rate of the cellulose is 91%.

Embodiment 2

[0095] 1. Preparation: Cut 20kg of kenaf husks into 0.5-1cm long and 1-1.5cm wide pieces for later use.

[0096] 2. Physical degumming: After boiling in a boiling water bath for 15 minutes, rinse the degummed kenaf husk fibers with tap water to remove the degummed products adhering to the fibers, and repeat three times.

[0097] 3. Steam explosion: Steam explosion conditions: 2.5MPa, 180s, dry and ready for use.

[0098] 4. Pulverization: Pulverize to 80 mesh with a pulverizer.

[0099] 5. Acid cellulase hydrolysis and saccharification: Completely saccharify cellulose, the substrate concentration is 3%, the acid cellulase activity is 700IU / ml, the pH value is 4.8, the enzyme-catalyzed reaction temperature is 55°C, and the reaction time is 6.5h, thus obtaining Reducing sugars obtained from the degradation of cellulose.

[0100] After the above process, the sugar conversion rate of the cellulose is 85%.

Embodiment 3

[0102] 1. Prepare 20kg of kenaf husks and cut them into 0.5-1cm long and 1-2cm wide pieces for later use.

[0103] 2. Physical degumming Boil in boiling water for 45 minutes.

[0104] 3. Wash hemp Rinse the degummed kenaf fiber with tap water to remove the degummed products adhering to the fiber.

[0105] 4. Steam explosion Steam explosion conditions: 3MPa, 100s, dry and ready for use.

[0106] 5. Pulverize with a plant pulverizer to 60 mesh.

[0107] 6. Acid cellulase hydrolysis and saccharification completely saccharifies cellulose, the substrate concentration is 3%, the acid cellulase activity is 1500IU / ml, the pH value is 4.8, the enzyme-catalyzed reaction temperature is 55°C, and the reaction time is 6.0h, thereby obtaining Reducing sugars from the degradation of cellulose.

[0108] After the above process, the sugar conversion rate of cellulose is 87%.

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Abstract

The invention provides a method for producing fermentable sugar through the zymohydrolysis of kenaf bast fiber, in particular the invention provides a method for preparing sugar through the degradation of cellulose, which comprises the following steps: physical degumming, steam blasting and zymohydrolysis are conducted to the kenaf bast fiber, so as to produce the sugar. The fermentable sugar produced by the method can be used for the production of fuel ethanol, and is characterized by short treatment time, high conversion rate, light pollution and the like.

Description

technical field [0001] The invention relates to the field of bioenergy, and more preferably, relates to a new method for enzymatically hydrolyzing kenaf husk fibers to produce fermentable sugars. The fermentable sugar produced by the method of the invention can be used in the production of fuel ethanol. Background technique [0002] At present, human beings have a great demand for energy, but resources such as petroleum are non-renewable resources, so people vigorously develop various new energy sources. [0003] Ethanol can be burned alone, and can also be used as a good gasoline oxygenate and high-octane blending component. It has been recognized as one of the most promising renewable clean energy sources. Its production and application have shown a high-speed development trend in the world. The fuel ethanol industry has become an important policy means and economic lever for governments to solve the shortage of oil resources, protect the quality of urban air environment,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P7/06
CPCY02E50/17Y02E50/10
Inventor 张士楚周龙海戴文魁李瑜
Owner SHANGHAI ZHONGWEI BIOCHEM
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