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Clavibacter and method for fermentation production of L-lysine

A technology of lysine and corynebacterium, applied in fermentation, bacteria, etc., can solve the problem of high production cost

Inactive Publication Date: 2005-04-20
上海新立工业微生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The second technical problem to be solved in the present invention is to disclose the method for producing L-lysine by fermenting the above strains, so as to overcome the high production cost defect of the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Fill 30ml of culture medium in a 500ml Erlenmeyer flask, which contains 175g / L glucose, 40g / L ammonium sulfate, 10ml / L cystine mother liquor (hair hydrolyzate), 5g / L corn steep liquor, 1g / L dihydrogen phosphate Potassium, 1g / L dipotassium hydrogen phosphate, 0.5g / L magnesium sulfate, 0.05g / L urea, 50g / L calcium carbonate (dissolved), pH 7.

[0090] The culture was sterilized at 120°C for 15 minutes. Take a ring of the parental strain and SNGBL 8 bacteria that have been pre-cultured for 24 hours from the slant of medium A, respectively, and insert them into the above-mentioned medium, and culture them with shaking at 32°C for 68 hours. The results are shown in the table below.

[0091] Strains used Characteristics Yield (g / L) * Conversion rate to sugar (%)

[0092] 2305 wild type 1.4 0.8

[0093] A111 HS - 、AEC r 58.1 33.2

[0094] SNGBL8 HS - 、AEC r 、MF r , 82.6 47.2

[0095] TA r

[0096] * The amount of L-lysine is calculated ...

Embodiment 2

[0098] Corn starch is hydrolyzed by double enzymes to make about 25% hydrolyzed sugar solution. Partially hydrolyzed sugar solution is concentrated into 55% sugar solution by vacuum thin film concentration device.

[0099] In two Erlenmeyer flasks with a volume of 5L, respectively fill 0.8L medium containing 30g / L corn starch hydrolyzed sugar liquid, 10g / L ammonium sulfate, 3g / L urea, 1g / L dihydrogen phosphate Potassium, 1g / L dipotassium hydrogen phosphate, 0.4g / L magnesium sulfate, 0.3g / L (based on nitrogen content) soybean meal hydrolyzate, 0.1mg / L thiamine hydrochloride, 0.03mg / L biotin. The culture was sterilized at 120°C for 15 minutes. A ring of the parental strain and SNGBL8 bacteria that had been pre-cultured for 24 hours was taken from the slant of medium A and inserted into the above-mentioned medium, and then cultured at 32°C for 14 hours. The above steps are for seed culture.

[0100] In two small fermentation tanks with a volume of 16L, each tank is filled with...

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PUM

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Abstract

The invention discloses a corynebacterium CGMCC No.0785 and a method for producing L-lysine by fermenting the strain. The method comprises the steps of first cultivating Corynebacterium CGMCCNo.0785, then fermenting in the culture medium, and then collecting L-lysine from the fermented liquid, the temperature is 25-35 DEG C, and the pH is 5.6-8.5. The production of lysine by adopting the strain of the invention can greatly increase the yield of lysine and the conversion rate of sugar, thereby significantly reducing the production cost of lysine.

Description

technical field [0001] The invention relates to a Corynebacterium species and a production method of L-lysine, in particular to a production method through microbial fermentation. Background technique [0002] L-Lysine is a very important amino acid, which is mainly used as feed additive, food additive and pharmaceutical industry raw material. It is generally produced by microbial fermentation. [0003] At present, many patents and documents have disclosed the method of producing L-lysine by microbial fermentation, such as the technology disclosed in the Japanese Patent Application No. 55213 / 1967 bulletin, which utilizes a kind of S-(2-amino Ethyl)-L-cysteine ​​(called AEC) resistant bacterium; As Japanese patent application No. 36888 / 1974 announcement utilizes a kind of AEC resistance and L-leucine, L-homoserine auxotroph type of variants; such as the Japanese patent application No. 19235 / 1981 announcement utilizes a gamma-methyllysine resistant variant. [0004] There i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/08
Inventor 富英华沈永喜劳含章
Owner 上海新立工业微生物科技有限公司
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